Abstract
A biofilm-forming strain of Pichia fermentans proved to be most effective in controlling brown rot on apple fruit when coinoculated into artificial wounds with a phytopathogenic isolate of Monilinia fructicola. Culture filtrates and autoclaved cells had no significant influence on the disease. When sprayed onto the apple fruit surface, this yeast formed a thin biofilm but failed to colonize the underlying tissues. When inoculated into wounds artificially inflicted to peach fruit or when sprayed onto the surface of peach fruit, the same strain showed an unexpected pathogenic behaviour, causing rapid decay of fruit tissues even in the absence of M. fructicola. Both optical and scanning electron microscopy were used to evaluate the pattern of fruit tissue colonization by P. fermentans. While on apple surface and within the apple wound the antagonist retained its yeast-like shape, colonization of peach fruit tissue was always characterized by a transition from budding growth to pseudohyphal growth. These results suggest that pseudohyphal growth plays a major role in governing the potential pathogenicity of P. fermentans, further emphasizing the importance of a thorough risk assessment for the safe use of any novel biocontrol agent.
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Claims 11
Claim · model-written summary
Pichia fermentans effectively controlled Monilinia brown rot in wounded apple fruit.
- Taxon
- apple
Malus domesticae0 - Treatment
- Pichia fermentanse0
- Interactor
- Monilinia fructicola
Monilinia fructicolae0pathogen - Second interactor
- Pichia fermentansPichia fermentanse0biocontrol_agent
- Outcome
- brown rote0
- Direction
- controllinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A biofilm-forming strain of Pichia fermentans proved to be most effective in controlling brown rot on apple fruit when coinoculated into artificial wounds with a phytopathogenic isolate of Monilinia fructicola.”
Claim · model-written summary
Culture filtrates did not significantly affect brown rot.
Not reportedTaxonComparator
Verbatim
e0“Culture filtrates and autoclaved cells had no significant influence on the disease.”
Claim · model-written summary
Autoclaved cells did not significantly affect brown rot.
Not reportedTaxonComparator
Verbatim
e0“Culture filtrates and autoclaved cells had no significant influence on the disease.”
Claim · model-written summary
Sprayed Pichia fermentans formed a thin surface biofilm on apple fruit.
- Taxon
- appleMalus domesticae0
- Organ / stage
- fruit surfacee0
- Outcome
- thin biofilme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When sprayed onto the apple fruit surface, this yeast formed a thin biofilm but failed to colonize the underlying tissues.”
Claim · model-written summary
Sprayed Pichia fermentans failed to colonize tissues beneath the apple fruit surface.
- Taxon
- appleMalus domesticae0
- Outcome
- colonize the underlying tissuese0
- Direction
- failede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When sprayed onto the apple fruit surface, this yeast formed a thin biofilm but failed to colonize the underlying tissues.”
Claim · model-written summary
Pichia fermentans caused rapid peach fruit decay after wound inoculation.
- Taxon
- peach
Prunus persicae0 - Treatment
- inoculated into woundse0
- Outcome
- rapid decay of fruit tissuese0
- Direction
- causinge0
Not reportedInteractorComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When inoculated into wounds artificially inflicted to peach fruit or when sprayed onto the surface of peach fruit, the same strain showed an unexpected pathogenic behaviour, causing rapid decay of fruit tissues even in the absence of M. fructicola.”
Claim · model-written summary
Pichia fermentans caused rapid peach fruit decay after surface spraying.
- Taxon
- peachPrunus persicae0
- Treatment
- sprayed onto the surfacee0
- Outcome
- rapid decay of fruit tissuese0
- Direction
- causinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When inoculated into wounds artificially inflicted to peach fruit or when sprayed onto the surface of peach fruit, the same strain showed an unexpected pathogenic behaviour, causing rapid decay of fruit tissues even in the absence of M. fructicola.”
Claim · model-written summary
Pichia fermentans retained a yeast-like shape on and within apple fruit wounds.
- Taxon
- appleMalus domesticae0
- Outcome
- yeast-like shapee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“While on apple surface and within the apple wound the antagonist retained its yeast-like shape”
Claim · model-written summary
Peach fruit colonization by Pichia fermentans involved a shift to pseudohyphal growth.
- Taxon
- peachPrunus persicae0
- Outcome
- transition from budding growth to pseudohyphal growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“colonization of peach fruit tissue was always characterized by a transition from budding growth to pseudohyphal growth.”
Claim · model-written summary
Authors suggest pseudohyphal growth governs Pichia fermentans pathogenic potential.
Not reportedTaxon
Verbatim
e0“These results suggest that pseudohyphal growth plays a major role in governing the potential pathogenicity of P. fermentans”
Claim · model-written summary
Authors recommend thorough risk assessment before use of novel biocontrol agents.
- Outcome
- thorough risk assessmente0
Not reportedTaxon
Verbatim
e0“further emphasizing the importance of a thorough risk assessment for the safe use of any novel biocontrol agent.”
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See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.