Abstract
Radiolabelled glucuronoxylan was formed by incubation of a Golgi membrane fraction from pea seedlings with UDP-( 14 C)GlcA and UDP-Xyl. Chelator-soluble glucuronoxylan was analysed by gel filtration on Sepharose CL-6B and CL-2B, and was resolved into a very high molecular weight peak (at least 7000 kDa) and a partially-excluded peak (50-75 kDa). Treatment of the latter peak with proteinase K caused a change in elution behaviour corresponding to the removal of a protein of 36-45 kDa. The association between polysaccharide and protein was not disrupted by high temperature or by high salt concentration, and was probably covalent
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Claims 7
Claim · model-written summary
Golgi membranes from pea seedlings formed radiolabelled glucuronoxylan.
- Taxon
- pea seedlings
Lathyrus oleraceuse0 - Organ / stage
- seedlingse0
- Outcome
- Radiolabelled glucuronoxylane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Radiolabelled glucuronoxylan was formed by incubation of a Golgi membrane fraction from pea seedlings with UDP-( 14 C)GlcA and UDP-Xyl”
Claim · model-written summary
Chelator-soluble glucuronoxylan had a very high molecular weight peak of at least 7000 kDa.
- Taxon
- pea seedlingsLathyrus oleraceuse1
- Outcome
- very high molecular weight peake0
- Value
- at least 7000 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chelator-soluble glucuronoxylan was analysed by gel filtration on Sepharose CL-6B and CL-2B, and was resolved into a very high molecular weight peak (at least 7000 kDa) and a partially-excluded peak (50-75 kDa)”
t0“plant Golgi apparatus”
e1“Radiolabelled glucuronoxylan was formed by incubation of a Golgi membrane fraction from pea seedlings with UDP-( 14 C)GlcA and UDP-Xyl”
Claim · model-written summary
Chelator-soluble glucuronoxylan had a partially excluded peak of 50-75 kDa.
- Taxon
- pea seedlingsLathyrus oleraceuse1
- Outcome
- partially-excluded peake0
- Value
- 50-75 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chelator-soluble glucuronoxylan was analysed by gel filtration on Sepharose CL-6B and CL-2B, and was resolved into a very high molecular weight peak (at least 7000 kDa) and a partially-excluded peak (50-75 kDa)”
t0“plant Golgi apparatus”
e1“Radiolabelled glucuronoxylan was formed by incubation of a Golgi membrane fraction from pea seedlings with UDP-( 14 C)GlcA and UDP-Xyl”
Claim · model-written summary
Proteinase K changed the elution of the smaller peak, consistent with removal of a 36-45 kDa protein.
- Taxon
- pea seedlingsLathyrus oleraceuse1
- Treatment
- proteinase Ke0
- Outcome
- elution behavioure0
- Direction
- changee0
- Value
- 36-45 kDae0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of the latter peak with proteinase K caused a change in elution behaviour corresponding to the removal of a protein of 36-45 kDa”
t0“plant Golgi apparatus”
e1“Radiolabelled glucuronoxylan was formed by incubation of a Golgi membrane fraction from pea seedlings with UDP-( 14 C)GlcA and UDP-Xyl”
Claim · model-written summary
High temperature did not disrupt the polysaccharide-protein association.
- Taxon
- pea seedlingsLathyrus oleraceuse1
- Treatment
- high temperaturee0
- Outcome
- association between polysaccharide and proteine0
- Direction
- not disruptede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The association between polysaccharide and protein was not disrupted by high temperature or by high salt concentration, and was probably covalent”
t0“plant Golgi apparatus”
e1“Radiolabelled glucuronoxylan was formed by incubation of a Golgi membrane fraction from pea seedlings with UDP-( 14 C)GlcA and UDP-Xyl”
Claim · model-written summary
High salt concentration did not disrupt the polysaccharide-protein association.
- Taxon
- pea seedlingsLathyrus oleraceuse1
- Treatment
- high salt concentratione0
- Outcome
- association between polysaccharide and proteine0
- Direction
- not disruptede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The association between polysaccharide and protein was not disrupted by high temperature or by high salt concentration, and was probably covalent”
t0“plant Golgi apparatus”
e1“Radiolabelled glucuronoxylan was formed by incubation of a Golgi membrane fraction from pea seedlings with UDP-( 14 C)GlcA and UDP-Xyl”
Claim · model-written summary
The polysaccharide-protein association was probably covalent.
- Taxon
- pea seedlingsLathyrus oleraceuse1
- Outcome
- association between polysaccharide and proteine0
- Qualifier
- probably covalente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The association between polysaccharide and protein was not disrupted by high temperature or by high salt concentration, and was probably covalent”
t0“plant Golgi apparatus”
e1“Radiolabelled glucuronoxylan was formed by incubation of a Golgi membrane fraction from pea seedlings with UDP-( 14 C)GlcA and UDP-Xyl”
The same questions in other papers
heat · quality: protein · Lathyrus oleraceus (pea)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- no effect
2 papers more in this group
See all 6 papers →salinity · quality: protein · Lathyrus oleraceus (pea)Results agree
3 papers in the same direction as this paper
- negative
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.