Abstract
The intracellular accumulation of organic compatible solutes functioning as osmoprotectants, such as polyols, is an important response mechanism of several plants to drought and salinity. In Olea europaea a mannitol transport system (OeMaT1) was previously characterized as a key player in plant response to salinity. In the present study, heterotrophic sink models, such as olive cell suspensions and fruit tissues, and source leaves were used for analytical, biochemical and molecular studies. The kinetic parameters of mannitol dehydrogenase (MTD) determined in cells growing in mannitol, at 25°C and pH 9.0, were as follows: K(m), 54.5 mM mannitol; and V(max), 0.47 μmol h⁻¹ mg⁻¹ protein. The corresponding cDNA was cloned and named OeMTD1. OeMTD1 expression was correlated with MTD activity, OeMaT1 expression and carrier-mediated mannitol transport in mannitol- and sucrose-grown cells. Furthermore, sucrose-grown cells displayed only residual OeMTD activity, even though high levels of OeMTD1 transcription were observed. There is evidence that OeMTD is regulated at both transcriptional and post-transcriptional levels. MTD activity and OeMTD1 expression were repressed after Na+, K+ and polyethylene glycol (PEG) treatments, in both mannitol- and sucrose-grown cells. In contrast, salt and drought significantly increased mannitol transport activity and OeMaT1 expression. Taken together, these studies support that olive trees cope with salinity and drought by coordinating mannitol transport with intracellular metabolism.
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Claims 14
Claim · model-written summary
MTD had K(m) of 54.5 mM mannitol.
- Taxon
- Olea europaea
Olea europaeat0 - Gene
- mannitol dehydrogenase (MTD)t1
- Outcome
- K(m)e0
- Value
- 54.5 mM mannitole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“K(m), 54.5 mM mannitol; and V(max), 0.47 μmol h⁻¹ mg⁻¹ protein”
t0“Olea europaea”
t1“mannitol dehydrogenase (MTD)”
Claim · model-written summary
MTD had V(max) of 0.47 μmol h⁻¹ mg⁻¹ protein.
- Taxon
- Olea europaeaOlea europaeat0
- Gene
- mannitol dehydrogenase (MTD)t1
- Outcome
- V(max)e0
- Value
- 0.47 μmol h⁻¹ mg⁻¹ proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“K(m), 54.5 mM mannitol; and V(max), 0.47 μmol h⁻¹ mg⁻¹ protein”
t0“Olea europaea”
t1“mannitol dehydrogenase (MTD)”
Claim · model-written summary
The corresponding cDNA was cloned and named OeMTD1.
- Taxon
- Olea europaeaOlea europaeat0
- Gene
- OeMTD1e0
- Outcome
- corresponding cDNA was clonede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The corresponding cDNA was cloned and named OeMTD1”
t0“Olea europaea”
t1“mannitol dehydrogenase (MTD)”
Claim · model-written summary
OeMTD1 expression correlated with MTD activity.
- Taxon
- Olea europaeaOlea europaeat0
- Organ / stage
- cellse0
- Gene
- OeMTD1e0
- Outcome
- MTD activitye0
- Qualifier
- correlatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OeMTD1 expression was correlated with MTD activity, OeMaT1 expression and carrier-mediated mannitol transport in mannitol- and sucrose-grown cells”
t0“Olea europaea”
t1“mannitol dehydrogenase (MTD)”
Claim · model-written summary
OeMTD1 expression correlated with OeMaT1 expression.
- Taxon
- Olea europaeaOlea europaeat0
- Gene
- OeMTD1e0
- Outcome
- OeMaT1 expressione0
- Qualifier
- correlatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OeMTD1 expression was correlated with MTD activity, OeMaT1 expression and carrier-mediated mannitol transport in mannitol- and sucrose-grown cells”
t0“Olea europaea”
t1“mannitol dehydrogenase (MTD)”
Claim · model-written summary
OeMTD1 expression correlated with carrier-mediated mannitol transport.
- Taxon
- Olea europaeaOlea europaeat0
- Gene
- OeMTD1e0
- Outcome
- carrier-mediated mannitol transporte0
- Qualifier
- correlatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OeMTD1 expression was correlated with MTD activity, OeMaT1 expression and carrier-mediated mannitol transport in mannitol- and sucrose-grown cells”
t0“Olea europaea”
t1“mannitol dehydrogenase (MTD)”
Claim · model-written summary
Sucrose-grown cells had only residual OeMTD activity despite high transcription.
- Taxon
- Olea europaeaOlea europaeat0
- Gene
- OeMTD1e0
- Outcome
- residual OeMTD activitye0
- Qualifier
- high levels of OeMTD1 transcriptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose-grown cells displayed only residual OeMTD activity, even though high levels of OeMTD1 transcription were observed”
t0“Olea europaea”
t1“mannitol dehydrogenase (MTD)”
Claim · model-written summary
OeMTD shows transcriptional and post-transcriptional regulation.
- Taxon
- Olea europaeaOlea europaeat0
- Gene
- OeMTDe0
- Outcome
- regulated at both transcriptional and post-transcriptional levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OeMTD is regulated at both transcriptional and post-transcriptional levels”
t0“Olea europaea”
t1“mannitol dehydrogenase (MTD)”
Claim · model-written summary
Na+ repressed MTD activity and OeMTD1 expression.
- Taxon
- Olea europaeaOlea europaeat0
- Treatment
- Na+e0
- Outcome
- MTD activity and OeMTD1 expressione0
- Direction
- repressede0
- Qualifier
- both mannitol- and sucrose-grown cellse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MTD activity and OeMTD1 expression were repressed after Na+, K+ and polyethylene glycol (PEG) treatments, in both mannitol- and sucrose-grown cells”
t0“Olea europaea”
t1“mannitol dehydrogenase (MTD)”
Claim · model-written summary
K+ repressed MTD activity and OeMTD1 expression.
- Taxon
- Olea europaeaOlea europaeat0
- Treatment
- K+e0
- Outcome
- MTD activity and OeMTD1 expressione0
- Direction
- repressede0
- Qualifier
- both mannitol- and sucrose-grown cellse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MTD activity and OeMTD1 expression were repressed after Na+, K+ and polyethylene glycol (PEG) treatments, in both mannitol- and sucrose-grown cells”
t0“Olea europaea”
t1“mannitol dehydrogenase (MTD)”
Claim · model-written summary
PEG repressed MTD activity and OeMTD1 expression.
- Taxon
- Olea europaeaOlea europaeat0
- Treatment
- polyethylene glycol (PEG)e0
- Outcome
- MTD activity and OeMTD1 expressione0
- Direction
- repressede0
- Qualifier
- both mannitol- and sucrose-grown cellse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MTD activity and OeMTD1 expression were repressed after Na+, K+ and polyethylene glycol (PEG) treatments, in both mannitol- and sucrose-grown cells”
t0“Olea europaea”
t1“mannitol dehydrogenase (MTD)”
Claim · model-written summary
Salt significantly increased mannitol transport activity and OeMaT1 expression.
- Taxon
- Olea europaeaOlea europaeat0
- Treatment
- salte0
- Outcome
- mannitol transport activity and OeMaT1 expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt and drought significantly increased mannitol transport activity and OeMaT1 expression”
t0“Olea europaea”
t1“mannitol dehydrogenase (MTD)”
Claim · model-written summary
Drought significantly increased mannitol transport activity and OeMaT1 expression.
- Taxon
- Olea europaeaOlea europaeat0
- Treatment
- droughte0
- Outcome
- mannitol transport activity and OeMaT1 expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt and drought significantly increased mannitol transport activity and OeMaT1 expression”
t0“Olea europaea”
t1“mannitol dehydrogenase (MTD)”
Claim · model-written summary
The authors infer olive stress coping involves coordinated mannitol transport and metabolism.
- Taxon
- olive treesOlea europaeae0
- Outcome
- cope with salinity and droughte0
- Qualifier
- coordinating mannitol transport with intracellular metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“olive trees cope with salinity and drought by coordinating mannitol transport with intracellular metabolism”
The same questions in other papers
drought · molecular: expression · Olea europaea (common olive)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.