Abstract
Floral organ shedding is a cell separation event preceded by cell-wall loosening and generally accompanied by cell expansion. Mutations in NEVERSHED (NEV) or INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) block floral organ abscission in Arabidopsis thaliana. NEV encodes an ADP-ribosylation factor GTPase-activating protein, and cells of nev mutant flowers display membrane-trafficking defects. IDA encodes a secreted peptide that signals through the receptor-like kinases HAESA (HAE) and HAESA-LIKE2 (HSL2). Analyses of single and double mutants revealed unique features of the nev and ida phenotypes. Cell-wall loosening was delayed in ida flowers. In contrast, nev and nev ida mutants displayed ectopic enlargement of abscission zone (AZ) cells, indicating that cell expansion alone is not sufficient to trigger organ loss. These results suggest that NEV initially prevents precocious cell expansion but is later integral for cell separation. IDA is involved primarily in the final cell separation step. A mutation in KNOTTED-LIKE FROM ARABIDOPSIS THALIANA1 (KNAT1), a suppressor of the ida mutant, could not rescue the abscission defects of nev mutant flowers, indicating that NEV-dependent activity downstream of KNAT1 is required. Transcriptional profiling of mutant AZs identified gene clusters regulated by IDA-HAE/HSL2. Several genes were more strongly downregulated in nev-7 compared with ida and hae hsl2 mutants, consistent with the rapid inhibition of organ loosening in nev mutants, and the overlapping roles of NEV and IDA in cell separation. A model of the crosstalk between the IDA signalling pathway and NEV-mediated membrane traffic during floral organ abscission is presented.
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Claims 8
Claim · model-written summary
Cell-wall loosening was delayed in ida flowers.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Material
- idae0
- Outcome
- Cell-wall looseninge0
- Direction
- delayede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cell-wall loosening was delayed in ida flowers.”
t0“Arabidopsis thaliana”
Claim · model-written summary
nev and nev ida mutants showed ectopic enlargement of abscission zone cells.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- nev and nev ida mutantse0
- Outcome
- abscission zone (AZ) cellse0
- Direction
- ectopic enlargemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nev and nev ida mutants displayed ectopic enlargement of abscission zone (AZ) cells”
t0“Arabidopsis thaliana”
Claim · model-written summary
NEV initially prevents precocious cell expansion.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- NEVe0
- Outcome
- precocious cell expansione0
- Qualifier
- initiallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NEV initially prevents precocious cell expansion but is later integral for cell separation.”
t0“Arabidopsis thaliana”
Claim · model-written summary
NEV is later integral for cell separation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- NEVe0
- Outcome
- cell separatione0
- Qualifier
- latere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NEV initially prevents precocious cell expansion but is later integral for cell separation.”
t0“Arabidopsis thaliana”
Claim · model-written summary
IDA is involved primarily in final cell separation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- IDAe0
- Outcome
- final cell separation stepe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IDA is involved primarily in the final cell separation step.”
t0“Arabidopsis thaliana”
Claim · model-written summary
KNAT1 mutation did not rescue nev abscission defects.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- A mutation in KNOTTED-LIKE FROM ARABIDOPSIS THALIANA1 (KNAT1)e0
- Gene
- KNAT1e0
- Outcome
- abscission defects of nev mutant flowerse0
- Direction
- could not rescuee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A mutation in KNOTTED-LIKE FROM ARABIDOPSIS THALIANA1 (KNAT1), a suppressor of the ida mutant, could not rescue the abscission defects of nev mutant flowers”
Claim · model-written summary
Mutant abscission-zone profiling identified gene clusters regulated by IDA-HAE/HSL2.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- IDA-HAE/HSL2e0
- Outcome
- gene clusters regulated by IDA-HAE/HSL2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptional profiling of mutant AZs identified gene clusters regulated by IDA-HAE/HSL2.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Several genes were more strongly downregulated in nev-7 than in ida and hae hsl2 mutants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- nev-7e0
- Outcome
- Several genese0
- Direction
- more strongly downregulatede0
- Comparator
- ida and hae hsl2 mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Several genes were more strongly downregulated in nev-7 compared with ida and hae hsl2 mutants”
t0“Arabidopsis thaliana”
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