Abstract
Previous results showed that expression of the gene encoding the LONG-CHAIN BASE2 (LCB(2)) subunit of serine palmitoyltransferase (SPT), designated BcLCB(2), from nonheading Chinese cabbage (Brassica campestris ssp. chinensis) was up-regulated during hypersensitive cell death (HCD) induced by the Phytophthora boehmeriae elicitor PB90. Overexpression of BcLCB(2) in Nicotiana tabacum leaves suppressed the HCD normally initiated by elicitors and PB90-triggered H(2)O(2) accumulation. BcLCB(2) also functioned as a suppressor of mouse Bcl-2 associated X (Bax) protein-mediated HCD and cell death caused by Ralstonia solanacearum. BcLCB(2) overexpression suppressed Bax- and oxidant stress-triggered yeast cell death. Reactive oxygen species (ROS) accumulation induced by Bax was compromised in BcLCB(2)-overexpressing yeast cells. The findings that NbLCB(2) silencing in Nicotiana benthamiana enhanced elicitor-triggered HCD, combined with the fact that myriocin, a potent inhibitor of SPT, had no effect on Bax-induced programmed cell death, suggested that suppression of cell death was not involved in the dominant-negative effect that resulted from BcLCB(2) overexpression. A BcLCB(2) mutant assay showed that the suppression was not involved in SPT activity. The results suggest that plant HCD and stress-induced yeast cell death might share a common signal transduction pathway involving LCB(2), and that LCB(2) protects against cell death by inhibiting ROS accumulation, this inhibition being independent of SPT activity.
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Claims 7
Claim · model-written summary
BcLCB(2) overexpression suppressed elicitor-induced hypersensitive cell death in tobacco leaves.
- Taxon
- Nicotiana tabacum
Nicotiana tabacume0 - Treatment
- Overexpression of BcLCB(2)e0
- Gene
- BcLCB(2)e0
- Outcome
- HCDe0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of BcLCB(2) in Nicotiana tabacum leaves suppressed the HCD normally initiated by elicitors”
Claim · model-written summary
BcLCB(2) overexpression suppressed PB90-triggered hydrogen peroxide accumulation in tobacco leaves.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- Overexpression of BcLCB(2)e0
- Gene
- BcLCB(2)e0
- Outcome
- PB90-triggered H(2)O(2) accumulatione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of BcLCB(2) in Nicotiana tabacum leaves suppressed the HCD normally initiated by elicitors and PB90-triggered H(2)O(2) accumulation”
Claim · model-written summary
BcLCB(2) suppressed Bax-mediated hypersensitive cell death.
- Taxon
- Brassica campestris ssp. chinensis
.jpg?width=60)
Brassica rapa subsp. oleiferat0 - Gene
- BcLCB(2)e0
- Outcome
- Bax) protein-mediated HCDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“BcLCB(2) also functioned as a suppressor of mouse Bcl-2 associated X (Bax) protein-mediated HCD”
t0“from nonheading Chinese cabbage (Brassica campestris ssp. chinensis)”
Claim · model-written summary
BcLCB(2) suppressed cell death caused by Ralstonia solanacearum.
- Taxon
- Brassica campestris ssp. chinensisBrassica rapa subsp. oleiferat0
- Interactor
- Ralstonia solanacearum
Ralstonia solanacearume0pathogen - Gene
- BcLCB(2)e0
- Outcome
- cell death caused by Ralstonia solanacearume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BcLCB(2) also functioned as a suppressor of mouse Bcl-2 associated X (Bax) protein-mediated HCD and cell death caused by Ralstonia solanacearum”
t0“from nonheading Chinese cabbage (Brassica campestris ssp. chinensis)”
Claim · model-written summary
Silencing NbLCB(2) enhanced elicitor-triggered hypersensitive cell death in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- NbLCB(2) silencinge0
- Gene
- NbLCB(2)e0
- Outcome
- elicitor-triggered HCDe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbLCB(2) silencing in Nicotiana benthamiana enhanced elicitor-triggered HCD”
Claim · model-written summary
Myriocin had no effect on Bax-induced programmed cell death.
- Taxon
- Brassica campestris ssp. chinensisBrassica rapa subsp. oleiferat0
- Treatment
- myriocine0
- Outcome
- Bax-induced programmed cell deathe0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“myriocin, a potent inhibitor of SPT, had no effect on Bax-induced programmed cell death”
t0“from nonheading Chinese cabbage (Brassica campestris ssp. chinensis)”
Claim · model-written summary
A BcLCB(2) mutant assay indicated that suppression did not involve SPT activity.
- Taxon
- Brassica campestris ssp. chinensisBrassica rapa subsp. oleiferat0
- Gene
- BcLCB(2)e0
- Outcome
- suppression was not involved in SPT activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A BcLCB(2) mutant assay showed that the suppression was not involved in SPT activity”
t0“from nonheading Chinese cabbage (Brassica campestris ssp. chinensis)”
The same questions in other papers
loss of function · cytology · Nicotiana benthamianaPositive and negative results
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See all 33 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.