Abstract
A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3 has been characterized. An increase in GAST1 RNA levels is detectable 2 h after treatment and levels continue to increase for at least an additional 10 h. Between 12 and 24 h following treatment, the amount of GAST1 RNA begins to decline and at 48 h the level is nearly equivalent to that of water-treated control plants. Nuclear runoff analysis indicates that 8 h after treatment with GA3, transcription of the GAST1 gene has increased only threefold, suggesting that GA acts both transcriptionally and post-transcriptionally. ABA partially inhibits the GA-mediated increase in GAST1 RNA abundance while ethephon, kinetin, and 2,4-D have little effect. GAST1 RNA is detectable in untreated leaves, stems, petioles and flowers, but not in roots. The GAST1 gene encodes a 0.7 kb transcript. The sequence of the GAST1 cDNA and genomic clones indicates that the gene is interrupted by three introns and potentially encodes a 112 amino acid protein of unknown function.
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Claims 13
Claim · model-written summary
GA3 spraying increased GAST1 RNA abundance more than 20-fold in shoots of the gib1 mutant
- Taxon
- tomato
Solanum lycopersicume1 - Material
- gib1e1
- Organ / stage
- shootse1
- Treatment
- spraying with GA3e1
- Gene
- GAST1e1
- Outcome
- abundancee1
- Direction
- increasese1
- Value
- > 20-folde1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3”
Claim · model-written summary
GAST1 RNA levels increase is detectable 2 h after GA3 spraying
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- spraying with GA3e1
- Gene
- GAST1e2
- Outcome
- GAST1 RNA levelse2
- Direction
- increasee2
- Value
- 2 he2
- Qualifier
- detectable 2 h after treatmente2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3”
e2“An increase in GAST1 RNA levels is detectable 2 h after treatment and levels continue to increase for at least an additional 10 h”
Claim · model-written summary
GAST1 RNA levels continue to increase for at least 10 h after GA3 spraying
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- spraying with GA3e1
- Gene
- GAST1e2
- Outcome
- GAST1 RNA levelse2
- Direction
- continue to increasee2
- Value
- at least an additional 10 he2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3”
e2“An increase in GAST1 RNA levels is detectable 2 h after treatment and levels continue to increase for at least an additional 10 h”
Claim · model-written summary
GAST1 RNA amount begins to decline between 12 and 24 h after GA3 spraying
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- spraying with GA3e1
- Gene
- GAST1e3
- Outcome
- amount of GAST1 RNAe3
- Direction
- declinee3
- Qualifier
- Between 12 and 24 h following treatmente3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3”
e3“Between 12 and 24 h following treatment, the amount of GAST1 RNA begins to decline and at 48 h the level is nearly equivalent to that of water-treated control plants”
Claim · model-written summary
At 48 h after GA3 spraying the GAST1 RNA level is nearly equivalent to water-treated control plants
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- spraying with GA3e1
- Gene
- GAST1e3
- Outcome
- levele3
- Direction
- nearly equivalente3
- Qualifier
- at 48 he3
- Comparator
- water-treated control plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3”
e3“Between 12 and 24 h following treatment, the amount of GAST1 RNA begins to decline and at 48 h the level is nearly equivalent to that of water-treated control plants”
Claim · model-written summary
GA3 treatment increased GAST1 gene transcription only threefold at 8 h
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- treatment with GA3e4
- Gene
- GAST1e4
- Outcome
- transcription of the GAST1 genee4
- Direction
- increasede4
- Value
- only threefolde4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3”
e4“Nuclear runoff analysis indicates that 8 h after treatment with GA3, transcription of the GAST1 gene has increased only threefold, suggesting that GA acts both transcriptionally and post-transcriptionally”
Claim · model-written summary
The authors suggest GA acts both transcriptionally and post-transcriptionally on GAST1
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- GA3e4
- Outcome
- transcription of the GAST1 genee4
- Qualifier
- GA acts both transcriptionally and post-transcriptionallye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3”
e4“Nuclear runoff analysis indicates that 8 h after treatment with GA3, transcription of the GAST1 gene has increased only threefold, suggesting that GA acts both transcriptionally and post-transcriptionally”
Claim · model-written summary
ABA partially inhibits the GA-mediated increase in GAST1 RNA abundance
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- ABAe5
- Gene
- GAST1e5
- Outcome
- GAST1 RNA abundancee5
- Direction
- partially inhibitse5
- Comparator
- GA-mediated increase in GAST1 RNA abundancee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3”
e5“ABA partially inhibits the GA-mediated increase in GAST1 RNA abundance while ethephon, kinetin, and 2,4-D have little effect”
Claim · model-written summary
Ethephon, kinetin and 2,4-D have little effect on GAST1 RNA abundance
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- ethephon, kinetin, and 2,4-De5
- Gene
- GAST1e5
- Outcome
- GAST1 RNA abundancee5
- Direction
- little effecte5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3”
e5“ABA partially inhibits the GA-mediated increase in GAST1 RNA abundance while ethephon, kinetin, and 2,4-D have little effect”
Claim · model-written summary
GAST1 RNA is detectable in untreated leaves, stems, petioles and flowers but not in roots
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- untreated leaves, stems, petioles and flowerse6
- Gene
- GAST1e6
- Outcome
- GAST1 RNAe6
- Qualifier
- but not in rootse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3”
e6“GAST1 RNA is detectable in untreated leaves, stems, petioles and flowers, but not in roots”
Claim · model-written summary
The GAST1 gene encodes a 0.7 kb transcript
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- GAST1e7
- Outcome
- transcripte7
- Value
- 0.7 kbe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3”
e7“The GAST1 gene encodes a 0.7 kb transcript”
Claim · model-written summary
The GAST1 gene is interrupted by three introns
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- GAST1e0
- Outcome
- intronse0
- Value
- threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3”
e0“The sequence of the GAST1 cDNA and genomic clones indicates that the gene is interrupted by three introns and potentially encodes a 112 amino acid protein of unknown function”
Claim · model-written summary
GAST1 potentially encodes a 112 amino acid protein of unknown function
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- GAST1e0
- Outcome
- proteine0
- Value
- 112 amino acide0
- Qualifier
- of unknown functione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3”
e0“The sequence of the GAST1 cDNA and genomic clones indicates that the gene is interrupted by three introns and potentially encodes a 112 amino acid protein of unknown function”
The same questions in other papers
2,4-D · pest · Solanum lycopersicum (tomato)Positive and negative results
1 in the opposite direction
- positive
(2-chloroethyl)phosphonic acid · pest · Solanum lycopersicum (tomato)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.