Abstract
In this report, a rapeseed (Brassica napus) haem oxygenase-1 gene BnHO1 was cloned and sequenced. It shared high homology with Arabidopsis HY1 proteins, and encodes a 32.6 kDa protein with a 54-amino-acid transit peptide, predicting the mature protein of 25.1 kDa. The mature BnHO1 expressed in Escherichia coli exhibits haem oxygenase (HO) activity. Furthermore, the application of lower doses of NaCl (10 mM) and polyethylene glycol (PEG) (2%) mimicked the inducible effects of naphthylacetic acid and the HO-1 inducer haemin on the up-regulation of BnHO1 and subsequent lateral root (LR) formation. Contrasting effects were observed when a higher dose of NaCl or PEG was applied. The above inducible and inhibitory responses were blocked significantly when the HO-1 inhibitor zinc protoporphyrin IX (ZnPPIX) or haemin was applied, both of which were reversed by the application of carbon monoxide or ZnPPIX, respectively. Moreover, the addition of ZnPPIX at different time points during LR formation indicated that BnHO1 might be involved in the early stages of LR formation. The auxin response factor transcripts and the auxin content in seedling roots were clearly induced by lower doses of salinity or osmotic stress. However, treatment with the inhibitor of polar auxin transport N-1-naphthylphthalamic acid prevented the above inducible responses conferred by lower doses of NaCl and PEG, which were further rescued when the treatments were combined with haemin. Taken together, these results suggested a novel role of the rapeseed HO-1 gene in salinity and osmotic stress-induced LR formation, with a possible interaction with auxin signalling.
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Claims 13
Claim · model-written summary
The rapeseed BnHO1 gene was cloned and sequenced.
Not reportedComparatorValue
Verbatim
e0“a rapeseed (Brassica napus) haem oxygenase-1 gene BnHO1 was cloned and sequenced”
Claim · model-written summary
BnHO1 showed high homology with Arabidopsis HY1 proteins.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Gene
- Arabidopsis HY1 proteinse0
- Outcome
- high homologye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“It shared high homology with Arabidopsis HY1 proteins”
t0“Brassica napus”
Claim · model-written summary
BnHO1 encodes a 32.6 kDa protein with a 54-amino-acid transit peptide and predicted mature mass of 25.1 kDa.
- Taxon
- Brassica napusBrassica napust0
- Gene
- BnHO1t0
- Outcome
- proteine0
- Value
- 32.6 kDae0
- Qualifier
- 54-amino-acid transit peptide, predicting the mature protein of 25.1 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“encodes a 32.6 kDa protein with a 54-amino-acid transit peptide, predicting the mature protein of 25.1 kDa”
t0“BnHO1, a haem oxygenase-1 gene from Brassica napus”
Claim · model-written summary
Mature BnHO1 expressed in Escherichia coli had haem oxygenase activity.
- Taxon
- Brassica napusBrassica napust0
- Gene
- BnHO1e0
- Outcome
- haem oxygenase (HO) activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mature BnHO1 expressed in Escherichia coli exhibits haem oxygenase (HO) activity”
t0“Brassica napus”
Claim · model-written summary
Low-dose NaCl upregulated BnHO1 and induced lateral root formation.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- NaCl (10 mM)e0
- Gene
- BnHO1e0
- Outcome
- up-regulation of BnHO1 and subsequent lateral root (LR) formatione0
- Direction
- up-regulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the application of lower doses of NaCl (10 mM) and polyethylene glycol (PEG) (2%) mimicked the inducible effects of naphthylacetic acid and the HO-1 inducer haemin on the up-regulation of BnHO1 and subsequent lateral root (LR) formation”
t0“Brassica napus”
Claim · model-written summary
Low-dose PEG upregulated BnHO1 and induced lateral root formation.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- polyethylene glycol (PEG) (2%)e0
- Gene
- BnHO1e0
- Outcome
- up-regulation of BnHO1 and subsequent lateral root (LR) formatione0
- Direction
- up-regulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the application of lower doses of NaCl (10 mM) and polyethylene glycol (PEG) (2%) mimicked the inducible effects of naphthylacetic acid and the HO-1 inducer haemin on the up-regulation of BnHO1 and subsequent lateral root (LR) formation”
t0“Brassica napus”
Claim · model-written summary
Higher doses of NaCl or PEG produced contrasting responses.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- a higher dose of NaCl or PEGe0
- Outcome
- Contrasting effectse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Contrasting effects were observed when a higher dose of NaCl or PEG was applied”
t0“Brassica napus”
Claim · model-written summary
ZnPPIX significantly blocked the induced and inhibited lateral-root responses.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- zinc protoporphyrin IX (ZnPPIX)e0
- Outcome
- inducible and inhibitory responsese0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The above inducible and inhibitory responses were blocked significantly when the HO-1 inhibitor zinc protoporphyrin IX (ZnPPIX) or haemin was applied”
t0“Brassica napus”
Claim · model-written summary
ZnPPIX timing experiments suggest BnHO1 acts during early lateral root formation.
- Taxon
- Brassica napusBrassica napust0
- Gene
- BnHO1e0
- Outcome
- early stages of LR formatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the addition of ZnPPIX at different time points during LR formation indicated that BnHO1 might be involved in the early stages of LR formation”
t0“Brassica napus”
Claim · model-written summary
Low salinity or osmotic stress induced auxin response factor transcripts and auxin content in seedling roots.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- seedling rootse0
- Treatment
- lower doses of salinity or osmotic stresse0
- Outcome
- auxin response factor transcripts and the auxin contente0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The auxin response factor transcripts and the auxin content in seedling roots were clearly induced by lower doses of salinity or osmotic stress”
t0“Brassica napus”
Claim · model-written summary
N-1-naphthylphthalamic acid prevented low-dose NaCl and PEG inducible responses.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- N-1-naphthylphthalamic acide0
- Outcome
- inducible responsese0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with the inhibitor of polar auxin transport N-1-naphthylphthalamic acid prevented the above inducible responses conferred by lower doses of NaCl and PEG”
t0“Brassica napus”
Claim · model-written summary
Haemin rescued the inducible responses blocked by the polar auxin transport inhibitor.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- haemine0
- Outcome
- rescuede0
- Direction
- rescuede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which were further rescued when the treatments were combined with haemin”
t0“Brassica napus”
Claim · model-written summary
The authors infer a role for rapeseed HO-1 in stress-induced lateral root formation with possible auxin signaling interaction.
Verbatim
e0“a novel role of the rapeseed HO-1 gene in salinity and osmotic stress-induced LR formation, with a possible interaction with auxin signalling”
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