Abstract
We have identified the function of the Arabidopsis DIMINUTO/DWARF1 (DIM/DWF1) gene by analyzing the dim mutant, a severe dwarf with greatly reduced fertility. Both the mutant phenotype and gene expression could be rescued by the addition of exogenous brassinolide. Analysis of endogenous sterols demonstrated that dim accumulates 24-methylenecholesterol but is deficient in campesterol, an early precursor of brassinolide. In addition, we show that dim is deficient in brassinosteroids as well. Feeding experiments using deuterium-labeled 24-methylenecholesterol and 24-methyldesmosterol confirmed that DIM/DWF1 is involved in both the isomerization and reduction of the Delta24(28) bond. This conversion is not required in cholesterol biosynthesis in animals but is a key step in the biosynthesis of plant sterols. Transient expression of a green fluorescent protein-DIM/DWF1 fusion protein and biochemical experiments showed that DIM/DWF1 is an integral membrane protein that most probably is associated with the endoplasmic reticulum.
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Claims 10
Claim · model-written summary
The dim mutant was severely dwarf and had greatly reduced fertility.
- Taxon
- Arabidopsis
Arabidopsise1 - Material
- dim mutante0
- Outcome
- severe dwarf with greatly reduced fertilitye0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dim mutant, a severe dwarf with greatly reduced fertility”
e1“Arabidopsis DIMINUTO/DWARF1”
Claim · model-written summary
Exogenous brassinolide rescued the dim mutant phenotype.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- addition of exogenous brassinolidee0
- Outcome
- mutant phenotypee0
- Direction
- rescuede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both the mutant phenotype and gene expression could be rescued by the addition of exogenous brassinolide”
e1“Arabidopsis DIMINUTO/DWARF1”
Claim · model-written summary
Exogenous brassinolide rescued gene expression in dim.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- addition of exogenous brassinolidee0
- Outcome
- gene expressione0
- Direction
- rescuede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both the mutant phenotype and gene expression could be rescued by the addition of exogenous brassinolide”
e1“Arabidopsis DIMINUTO/DWARF1”
Claim · model-written summary
The dim mutant accumulated 24-methylenecholesterol.
- Taxon
- ArabidopsisArabidopsise1
- Material
- dime0
- Outcome
- 24-methylenecholesterole0
- Direction
- accumulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dim accumulates 24-methylenecholesterol but is deficient in campesterol”
e1“Arabidopsis DIMINUTO/DWARF1”
Claim · model-written summary
The dim mutant was deficient in campesterol.
- Taxon
- ArabidopsisArabidopsise1
- Material
- dime0
- Outcome
- campesterole0
- Direction
- deficiente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dim accumulates 24-methylenecholesterol but is deficient in campesterol”
e1“Arabidopsis DIMINUTO/DWARF1”
Claim · model-written summary
The dim mutant was deficient in brassinosteroids.
- Taxon
- ArabidopsisArabidopsise1
- Material
- dime0
- Outcome
- brassinosteroidse0
- Direction
- deficiente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dim is deficient in brassinosteroids as well”
e1“Arabidopsis DIMINUTO/DWARF1”
Claim · model-written summary
Feeding experiments showed DIM/DWF1 involvement in isomerization of the Delta24(28) bond.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- DIM/DWF1e0
- Outcome
- isomerizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Feeding experiments using deuterium-labeled 24-methylenecholesterol and 24-methyldesmosterol confirmed that DIM/DWF1 is involved in both the isomerization and reduction of the Delta24(28) bond”
e1“Arabidopsis DIMINUTO/DWARF1”
Claim · model-written summary
Feeding experiments showed DIM/DWF1 involvement in reduction of the Delta24(28) bond.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- DIM/DWF1e0
- Outcome
- reduction of the Delta24(28) bonde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Feeding experiments using deuterium-labeled 24-methylenecholesterol and 24-methyldesmosterol confirmed that DIM/DWF1 is involved in both the isomerization and reduction of the Delta24(28) bond”
e1“Arabidopsis DIMINUTO/DWARF1”
Claim · model-written summary
DIM/DWF1 was identified as an integral membrane protein.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- DIM/DWF1e0
- Outcome
- integral membrane proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DIM/DWF1 is an integral membrane protein that most probably is associated with the endoplasmic reticulum”
e1“Arabidopsis DIMINUTO/DWARF1”
Claim · model-written summary
DIM/DWF1 was probably associated with the endoplasmic reticulum.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- DIM/DWF1e0
- Outcome
- associated with the endoplasmic reticulume0
- Qualifier
- most probablye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DIM/DWF1 is an integral membrane protein that most probably is associated with the endoplasmic reticulum”
e1“Arabidopsis DIMINUTO/DWARF1”
The same questions in other papers
brassinolide · molecular: expression · ArabidopsisPositive and negative results
7 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- no effect
- no effect
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.