Abstract
An efficient protocol was developed for in vitro morphogenic ability along with plantlet regeneration of two Bangladeshi indica rice varieties (BR24 and BR26) via somatic embryogenesis by applying 50 mM NaCl stress in callus induction and suspension initiation media. Osmotic stress was induced by NaCl (50, 100, 150, 200 and 250 mM) on the cell growth in suspension maintenance media. In viability test stress adapted cells showed 85 - 95% viability up to 200 mM NaCl compared with stress shocked (MS1-50) and control (MS1-0) treatments. Higher stress adapted cells showed growth retardation and the induction of plasmolysis. For both genotypes somatic embryos were obtained in both MS based liquid and semisolid media with or without 50 and 100 mM NaCl. Cell suspension-derived micro-calli were partially desiccated (6 - 12 hr) and subsequently maintained in MS1 callus induction media supplemented with proline (12 mM), ABA (2 mg/l) and 0.6% phytagel in the presence or absence of 50 and 100 mM NaCl. Subsequently, desiccated somatic embryos were transferred in MS based regeneration media with or without 50 and 100 mM NaCl. Proline mediated callus was found to be more effective in embryo differentiation than ABA. Partial desiccation dramatically enhanced callus growth and partially increased regeneration percentage. BR24 showed a better regeneration response producing plantlets in presence of proline in control media while BR26 restored regeneration potential in the presence of ABA and 100 mM NaCl. Plantlets regenerated from salt stressed callus cultures were then grown in compost in a glasshouse and produced normal, fertile plants. Key words: Indica rice, Cell suspension, Morphogenic, Regeneration D.O.I. 10.3329/ptcb.v19i2.5436 Plant Tissue Cult. & Biotech. 19(2): 185-197, 2009 (December)
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Claims 10
Claim · model-written summary
Stress adapted rice cells showed 85-95% viability up to 200 mM NaCl compared with stress shocked and control treatments.
- Taxon
- indica rice
Oryza sativa Indica Groupe0 - Treatment
- stress adaptede1
- Outcome
- viabilitye1
- Value
- 85 - 95%e1
- Qualifier
- up to 200 mM NaCle1
- Comparator
- stress shocked (MS1-50) and control (MS1-0) treatmentse1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two Bangladeshi indica rice varieties (BR24 and BR26)”
e1“In viability test stress adapted cells showed 85 - 95% viability up to 200 mM NaCl compared with stress shocked (MS1-50) and control (MS1-0) treatments”
Claim · model-written summary
Stress adapted rice cells showed growth retardation.
- Taxon
- indica riceOryza sativa Indica Groupe0
- Treatment
- stress adapted cellse2
- Outcome
- growthe2
- Direction
- retardatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two Bangladeshi indica rice varieties (BR24 and BR26)”
e2“Higher stress adapted cells showed growth retardation and the induction of plasmolysis”
Claim · model-written summary
Stress adapted rice cells showed induction of plasmolysis.
- Taxon
- indica riceOryza sativa Indica Groupe0
- Treatment
- stress adapted cellse2
- Outcome
- plasmolysise2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two Bangladeshi indica rice varieties (BR24 and BR26)”
e2“Higher stress adapted cells showed growth retardation and the induction of plasmolysis”
Claim · model-written summary
Somatic embryos of indica rice were obtained with or without 50 and 100 mM NaCl.
- Taxon
- indica riceOryza sativa Indica Groupe0
- Outcome
- somatic embryose3
- Qualifier
- with or without 50 and 100 mM NaCle3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two Bangladeshi indica rice varieties (BR24 and BR26)”
e3“For both genotypes somatic embryos were obtained in both MS based liquid and semisolid media with or without 50 and 100 mM NaCl”
Claim · model-written summary
Proline was more effective than ABA in embryo differentiation of indica rice callus.
- Taxon
- indica riceOryza sativa Indica Groupe0
- Treatment
- Prolinee4
- Outcome
- embryo differentiatione4
- Direction
- more effectivee4
- Comparator
- ABAe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two Bangladeshi indica rice varieties (BR24 and BR26)”
e4“Proline mediated callus was found to be more effective in embryo differentiation than ABA”
Claim · model-written summary
Partial desiccation dramatically enhanced callus growth of indica rice.
- Taxon
- indica riceOryza sativa Indica Groupe0
- Treatment
- Partial desiccatione5
- Outcome
- callus growthe5
- Direction
- enhancede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two Bangladeshi indica rice varieties (BR24 and BR26)”
e5“Partial desiccation dramatically enhanced callus growth and partially increased regeneration percentage”
Claim · model-written summary
Partial desiccation partially increased regeneration percentage of indica rice.
- Taxon
- indica riceOryza sativa Indica Groupe0
- Treatment
- Partial desiccatione5
- Outcome
- regeneration percentagee5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two Bangladeshi indica rice varieties (BR24 and BR26)”
e5“Partial desiccation dramatically enhanced callus growth and partially increased regeneration percentage”
Claim · model-written summary
BR24 showed a better regeneration response producing plantlets with proline in control media.
- Taxon
- indica riceOryza sativa Indica Groupe0
- Material
- BR24e6
- Treatment
- prolinee6
- Outcome
- regeneration responsee6
- Direction
- bettere6
- Qualifier
- producing plantletse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two Bangladeshi indica rice varieties (BR24 and BR26)”
e6“BR24 showed a better regeneration response producing plantlets in presence of proline in control media”
Claim · model-written summary
BR26 restored regeneration potential in the presence of ABA and 100 mM NaCl.
- Taxon
- indica riceOryza sativa Indica Groupe0
- Material
- BR26e7
- Treatment
- ABA and 100 mM NaCle7
- Outcome
- regeneration potentiale7
- Direction
- restorede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two Bangladeshi indica rice varieties (BR24 and BR26)”
e7“BR26 restored regeneration potential in the presence of ABA and 100 mM NaCl”
Claim · model-written summary
Plantlets regenerated from salt stressed callus grew into normal, fertile plants in a glasshouse.
- Taxon
- indica riceOryza sativa Indica Groupe0
- Outcome
- normal, fertile plantse1
- Setting
- in a glasshousee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two Bangladeshi indica rice varieties (BR24 and BR26)”
e1“Plantlets regenerated from salt stressed callus cultures were then grown in compost in a glasshouse and produced normal, fertile plants”
The same questions in other papers
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- no effect
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See all 282 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.