Abstract
The chromomethylase (CMT) protein family is unique to plants and controls non-CpG methylation. Here, we investigated the developmental expression of CMT3-2 in Nicotiana benthamiana (NbCMT3-2) and its significance by analyzing plants with silenced NbCMT3-2 and leaf tissues transiently expressing the N-terminal polypeptide. Alignment of the NbCMT3-2 amino acid sequence with that of other plant CMT3s showed a specific N-terminal extension required for nuclear localization. Transient expression of the N-terminal polypeptide in N. benthamiana resulted in chlorotic lesions. NbCMT3-2 was expressed mainly in proliferating tissues such as the shoot apex and developing leaves. We generated transgenic N. benthamiana harboring a fusion reporter construct linking the NbCMT3-2 promoter region and the β-glucuronidase (GUS) reporter (pNbCMT3-2::GUS) to analyze the tissue-specific expression of NbCMT3-2. NbCMT3-2 was expressed in the shoot and root apical meristem and leaf primordia in young seedlings and highly expressed in developing leaves and ovary as well as lateral buds in mature plants. Virus-induced gene silencing used to knock down the expression of NbCMT3 or NbCMT3-2 or both led to partial loss of genomic DNA methylation. Plants with suppressed NbCMT3 expression grew and developed normally, whereas leaves with NbCMT3-2 knockdown showed mild curling as compared with controls. Silencing NbCMT3/3-2 severely interfered with leaf development and directly or indirectly affected the expression of genes involved in jasmonate homeostasis. The differential roles of NbCMT3 and NbCMT3-2 were investigated and compared. We reveal the expression patterns of NbCMT3-2 in proliferating tissues. NbCMT3-2 may play an essential role in leaf development by modulating jasmonate pathways.
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Claims 10
Claim · model-written summary
The NbCMT3-2 N-terminal extension was required for nuclear localization.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Gene
- NbCMT3-2e0
- Outcome
- nuclear localizatione0
- Qualifier
- specific N-terminal extensione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alignment of the NbCMT3-2 amino acid sequence with that of other plant CMT3s showed a specific N-terminal extension required for nuclear localization”
t0“Nicotiana benthamiana”
Claim · model-written summary
Transient expression of the N-terminal polypeptide produced chlorotic lesions.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- Transient expression of the N-terminal polypeptidee0
- Outcome
- chlorotic lesionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of the N-terminal polypeptide in N. benthamiana resulted in chlorotic lesions”
Claim · model-written summary
NbCMT3-2 was expressed mainly in proliferating tissues.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Organ / stage
- shoot apex and developing leavese0
- Gene
- NbCMT3-2e0
- Outcome
- expressed mainly in proliferating tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbCMT3-2 was expressed mainly in proliferating tissues such as the shoot apex and developing leaves”
t0“Nicotiana benthamiana”
Claim · model-written summary
In young seedlings, NbCMT3-2 was expressed in shoot and root apical meristems and leaf primordia.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Organ / stage
- young seedlingse0
- Gene
- NbCMT3-2e0
- Outcome
- expressed in the shoot and root apical meristem and leaf primordiae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbCMT3-2 was expressed in the shoot and root apical meristem and leaf primordia in young seedlings”
t0“Nicotiana benthamiana”
Claim · model-written summary
In mature plants, NbCMT3-2 expression was high in developing leaves, ovary, and lateral buds.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Organ / stage
- mature plantse0
- Gene
- NbCMT3-2e0
- Outcome
- highly expressed in developing leaves and ovary as well as lateral budse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbCMT3-2 was expressed in the shoot and root apical meristem and leaf primordia in young seedlings and highly expressed in developing leaves and ovary as well as lateral buds in mature plants”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbCMT3, NbCMT3-2, or both partly reduced genomic DNA methylation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Virus-induced gene silencinge0
- Gene
- NbCMT3-2e0
- Outcome
- genomic DNA methylatione0
- Direction
- partial losse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Virus-induced gene silencing used to knock down the expression of NbCMT3 or NbCMT3-2 or both led to partial loss of genomic DNA methylation”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbCMT3 suppression did not alter normal growth and development.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbCMT3e0
- Outcome
- grew and developed normallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants with suppressed NbCMT3 expression grew and developed normally”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbCMT3-2 knockdown leaves showed mild curling versus controls.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbCMT3-2 knockdowne0
- Gene
- NbCMT3-2e0
- Outcome
- mild curlinge0
- Comparator
- controlse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“leaves with NbCMT3-2 knockdown showed mild curling as compared with controls”
t0“Nicotiana benthamiana”
Claim · model-written summary
Combined NbCMT3/3-2 silencing severely interfered with leaf development.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing NbCMT3/3-2e0
- Gene
- NbCMT3/3-2e0
- Outcome
- leaf developmente0
- Direction
- severely interferede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing NbCMT3/3-2 severely interfered with leaf development”
t0“Nicotiana benthamiana”
Claim · model-written summary
Combined silencing affected expression of genes involved in jasmonate homeostasis.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing NbCMT3/3-2e0
- Gene
- NbCMT3/3-2e0
- Outcome
- expression of genes involved in jasmonate homeostasise0
- Qualifier
- directly or indirectlye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing NbCMT3/3-2 severely interfered with leaf development and directly or indirectly affected the expression of genes involved in jasmonate homeostasis”
t0“Nicotiana benthamiana”
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See all 43 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.