Abstract
We studied the expression of three promoter 5' deletion constructs (-218, -599, and -1312) of the LEA (late embryogenesis abundant)-class gene Dc3 fused to beta-glucuronidase (GUS), where each construct value refers to the number of base pairs upstream of the transcription start site at which the deletion occurred. The Dc3 gene is noted for its induction by abscisic acid (ABA), but its response to other plant hormones and various environmental stresses has not been reported previously for vegetative cells. Fourteen-day-old transgenic tobacco (Nicotiana tabacum L.) seedlings were exposed to dehydration, hypoxia, salinity, exogenous ethylene, or exogenous methyl jasmonate (MeJa). GUS activity was quantified fluorimetrically and expression was observed by histochemical staining of the seedlings. An increase in GUS activity was observed in plants with constructs -599 and -1312 in response to dehydration and salinity within 6 h of stress, and at 12 h in response to hypoxia. No increase in endogenous ABA was found in any of the three lines, even after 72 h of hypoxia. An ABA-independent increase in GUS activity was observed when endogenous ABA biosynthesis was blocked by fluridone and plants were exposed to 5 &mgr;L L-1 ethylene in air or 100 &mgr;M MeJa. Virtually no expression was observed in construct -218 in response to dehydration, salinity, or MeJa, but there was a moderate response to ethylene and hypoxia. This suggests that the region between -218 and -599 is necessary for ABA (dehydration and salinity)- and MeJa-dependent expression, whereas ethylene-mediated expression does not require this region of the promoter.
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Claims 9
Claim · model-written summary
Dehydration and salinity increased GUS activity in -599 and -1312 tobacco constructs within 6 h.
- Taxon
- transgenic tobacco
Nicotiana tabacumt0 - Material
- constructs -599 and -1312e0
- Treatment
- dehydration and salinitye0
- Gene
- GUSe0
- Outcome
- GUS activitye0
- Direction
- increasee0
- Qualifier
- within 6 h of stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An increase in GUS activity was observed in plants with constructs -599 and -1312 in response to dehydration and salinity within 6 h of stress”
t0“transgenic tobacco (Nicotiana tabacum L.) seedlings”
Claim · model-written summary
Hypoxia increased GUS activity in -599 and -1312 constructs at 12 h.
- Taxon
- transgenic tobaccoNicotiana tabacumt0
- Material
- constructs -599 and -1312e0
- Treatment
- hypoxiae0
- Gene
- GUSe0
- Outcome
- GUS activitye0
- Direction
- increasee0
- Qualifier
- at 12 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An increase in GUS activity was observed in plants with constructs -599 and -1312 in response to dehydration and salinity within 6 h of stress, and at 12 h in response to hypoxia”
t0“transgenic tobacco (Nicotiana tabacum L.) seedlings”
Claim · model-written summary
Endogenous ABA did not increase in any line after hypoxia.
- Taxon
- transgenic tobaccoNicotiana tabacumt0
- Treatment
- hypoxiae0
- Outcome
- endogenous ABAe0
- Direction
- No increasee0
- Qualifier
- even after 72 h of hypoxiae0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No increase in endogenous ABA was found in any of the three lines, even after 72 h of hypoxia”
t0“transgenic tobacco (Nicotiana tabacum L.) seedlings”
Claim · model-written summary
Ethylene raised GUS activity despite blocked ABA biosynthesis.
- Taxon
- transgenic tobaccoNicotiana tabacumt0
- Treatment
- 5 &mgr;L L-1 ethylene in aire0
- Gene
- GUSe0
- Outcome
- GUS activitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An ABA-independent increase in GUS activity was observed when endogenous ABA biosynthesis was blocked by fluridone and plants were exposed to 5 &mgr;L L-1 ethylene in air or 100 &mgr;M MeJa”
t0“transgenic tobacco (Nicotiana tabacum L.) seedlings”
Claim · model-written summary
MeJa raised GUS activity despite blocked ABA biosynthesis.
- Taxon
- transgenic tobaccoNicotiana tabacumt0
- Treatment
- 100 &mgr;M MeJae0
- Gene
- GUSe0
- Outcome
- GUS activitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An ABA-independent increase in GUS activity was observed when endogenous ABA biosynthesis was blocked by fluridone and plants were exposed to 5 &mgr;L L-1 ethylene in air or 100 &mgr;M MeJa”
t0“transgenic tobacco (Nicotiana tabacum L.) seedlings”
Claim · model-written summary
Construct -218 showed virtually no expression under dehydration, salinity or MeJa.
- Taxon
- transgenic tobaccoNicotiana tabacumt0
- Material
- construct -218e0
- Treatment
- dehydration, salinity, or MeJae0
- Outcome
- expressione0
- Direction
- Virtually noe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Virtually no expression was observed in construct -218 in response to dehydration, salinity, or MeJa”
t0“transgenic tobacco (Nicotiana tabacum L.) seedlings”
Claim · model-written summary
Construct -218 responded moderately to ethylene and hypoxia.
- Taxon
- transgenic tobaccoNicotiana tabacumt0
- Material
- construct -218t1
- Treatment
- ethylene and hypoxiae0
- Outcome
- moderate responsee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was a moderate response to ethylene and hypoxia”
t0“transgenic tobacco (Nicotiana tabacum L.) seedlings”
t1“construct -218”
Claim · model-written summary
The -218 to -599 promoter region is necessary for ABA and MeJa dependent expression.
- Taxon
- transgenic tobaccoNicotiana tabacumt0
- Outcome
- MeJa-dependent expressione0
- Qualifier
- the region between -218 and -599e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the region between -218 and -599 is necessary for ABA (dehydration and salinity)- and MeJa-dependent expression”
t0“transgenic tobacco (Nicotiana tabacum L.) seedlings”
Claim · model-written summary
Ethylene mediated expression does not require the -218 to -599 region.
- Taxon
- transgenic tobaccoNicotiana tabacumt0
- Outcome
- ethylene-mediated expressione0
- Qualifier
- this region of the promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene-mediated expression does not require this region of the promoter”
t0“transgenic tobacco (Nicotiana tabacum L.) seedlings”
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