Abstract
The occurrence and roles of cGMP were investigated in aleurone layers and protoplasts isolated from barley (cv Himalaya) grain. Levels of cGMP in freshly isolated barley aleurone layers ranged from 0.065 to 0.08 pmol/g fresh weight of tissue, and cGMP levels increased transiently after incubation in gibberellic acid (GA). Abscisic acid (ABA) did not increase cGMP levels in aleurone layers. LY 83583 (LY), an inhibitor of guanylyl cyclase, prevented the GA-induced increase in cGMP and inhibited GA-induced a-amylase synthesis and secretion. The inhibitory effectsof LY could be overcome by membranepermeant analogs of cGMP. LY also prevented GA-induced accumulation of a-amylase and GAMYB mRNAs. cGMP alone was not sufficient to induce the accumulation of a-amylase or GAMYB mRNA. LY had a less dramatic effect on the accumulation of mRNAs encoding the ABA-responsive gene Rab21. We conclude that cGMP plays an important role in GA, but not ABA, signaling in the barley aleurone cell.
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Claims 9
Claim · model-written summary
Barley aleurone layers had cGMP levels of 0.065 to 0.08 pmol/g fresh weight.
- Taxon
- barley
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Hordeum vulgaree0 - Material
- cv Himalayae0
- Organ / stage
- aleurone layerse1
- Outcome
- Levels of cGMPe1
- Value
- 0.065 to 0.08 pmol/g fresh weighte1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The occurrence and roles of cGMP were investigated in aleurone layers and protoplasts isolated from barley (cv Himalaya) grain”
e1“Levels of cGMP in freshly isolated barley aleurone layers ranged from 0.065 to 0.08 pmol/g fresh weight of tissue”
Claim · model-written summary
Gibberellic acid increased cGMP levels transiently in barley aleurone layers.
- Taxon
- barleyHordeum vulgaree0
- Material
- cv Himalayae0
- Treatment
- gibberellic acid (GA)e2
- Outcome
- cGMP levelse2
- Direction
- increasede2
- Qualifier
- transientlye2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The occurrence and roles of cGMP were investigated in aleurone layers and protoplasts isolated from barley (cv Himalaya) grain”
e2“cGMP levels increased transiently after incubation in gibberellic acid (GA)”
Claim · model-written summary
Abscisic acid did not increase cGMP levels in barley aleurone layers.
- Taxon
- barleyHordeum vulgaree0
- Material
- cv Himalayae0
- Organ / stage
- aleurone layerse3
- Treatment
- Abscisic acid (ABA)e3
- Outcome
- cGMP levelse3
- Direction
- did not increasee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The occurrence and roles of cGMP were investigated in aleurone layers and protoplasts isolated from barley (cv Himalaya) grain”
e3“Abscisic acid (ABA) did not increase cGMP levels in aleurone layers”
Claim · model-written summary
LY 83583, an inhibitor of guanylyl cyclase, prevented the GA-induced increase in cGMP in barley aleurone layers.
- Taxon
- barleyHordeum vulgaree0
- Material
- cv Himalayae0
- Treatment
- LY 83583 (LY)e4
- Outcome
- GA-induced increase in cGMPe4
- Direction
- preventede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The occurrence and roles of cGMP were investigated in aleurone layers and protoplasts isolated from barley (cv Himalaya) grain”
e4“LY 83583 (LY), an inhibitor of guanylyl cyclase, prevented the GA-induced increase in cGMP”
Claim · model-written summary
LY 83583 inhibited GA-induced a-amylase synthesis and secretion in barley aleurone layers.
- Taxon
- barleyHordeum vulgaree0
- Material
- cv Himalayae0
- Treatment
- LY 83583 (LY)e4
- Outcome
- a-amylase synthesis and secretione5
- Direction
- inhibitede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The occurrence and roles of cGMP were investigated in aleurone layers and protoplasts isolated from barley (cv Himalaya) grain”
e4“LY 83583 (LY), an inhibitor of guanylyl cyclase, prevented the GA-induced increase in cGMP”
e5“inhibited GA-induced a-amylase synthesis and secretion”
Claim · model-written summary
LY prevented GA-induced accumulation of a-amylase and GAMYB mRNAs in barley aleurone layers.
- Taxon
- barleyHordeum vulgaree0
- Material
- cv Himalayae0
- Treatment
- LYe6
- Gene
- GAMYBe6
- Outcome
- accumulation of a-amylase and GAMYB mRNAse6
- Direction
- preventede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The occurrence and roles of cGMP were investigated in aleurone layers and protoplasts isolated from barley (cv Himalaya) grain”
e6“LY also prevented GA-induced accumulation of a-amylase and GAMYB mRNAs”
Claim · model-written summary
cGMP alone was not sufficient to induce accumulation of a-amylase or GAMYB mRNA in barley aleurone layers.
- Taxon
- barleyHordeum vulgaree0
- Material
- cv Himalayae0
- Treatment
- cGMP alonee7
- Gene
- GAMYBe7
- Outcome
- accumulation of a-amylase or GAMYB mRNAe7
- Direction
- not sufficient to inducee7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The occurrence and roles of cGMP were investigated in aleurone layers and protoplasts isolated from barley (cv Himalaya) grain”
e7“cGMP alone was not sufficient to induce the accumulation of a-amylase or GAMYB mRNA”
Claim · model-written summary
LY had a less dramatic effect on accumulation of mRNAs encoding the ABA-responsive gene Rab21 in barley aleurone layers.
- Taxon
- barleyHordeum vulgaree0
- Material
- cv Himalayae0
- Treatment
- LYe1
- Gene
- Rab21e1
- Outcome
- accumulation of mRNAs encoding the ABA-responsive gene Rab21e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The occurrence and roles of cGMP were investigated in aleurone layers and protoplasts isolated from barley (cv Himalaya) grain”
e1“LY had a less dramatic effect on the accumulation of mRNAs encoding the ABA-responsive gene Rab21”
Claim · model-written summary
cGMP plays an important role in GA signaling, but not ABA signaling, in barley aleurone cells.
- Taxon
- barleyHordeum vulgaree1
- Compound
- cGMPe1
- Outcome
- important role in GA, but not ABA, signalinge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We conclude that cGMP plays an important role in GA, but not ABA, signaling in the barley aleurone cell”
The same questions in other papers
(+)-abscisic acid · molecular: metabolites · Hordeum vulgare (barley)Positive and negative results
6 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
11 papers more in this group
See all 15 papers →3',5'-cyclic GMP · molecular: expression · Hordeum vulgare (barley)Results agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.