Paper · 1992 · Journal of the American Society for Horticultural Science

Involvement of Polyamides in Auxin and Agrobacterium rhizogenes - induced Rooting of Fruit Trees in Vitro

Eddo Rugini

11
claims
11
verbatim quotes
38
citations
5
research areas
✓
Open access

Abstract

In the olive Olea europaea (L.), the polyamides (PAs), putrescine, spermidine, and spermine, when added exogenously at a concentration of 1 m m in the in vitro rooting medium, combined with 5 μ m auxin concentration, promoted early rooting and increased the final rooting percentage and the number of roots per explant. The effect was less evident in olive explants rooted with the basal blanching method; thin-layer chromatography of total endogenous PAs in `these explants revealed lower levels on day 2 compared with controls, while by day 5 PA concentrations in both had fallen to similar levels. Furthermore, putrescine decreased the pH of the medium by 0.5 units around the explants. PAs had little effect on apple Malus pumila (Mill.) and no effect on almond Prunus dulcis (Miller) D.A. Webb and pistachio Pistacia vera (L.). There were also some positive effects observed, but only in olive, when rooting was induced by Agrobacterium rhizogenes in auxin-free medium. Few plantlets showed agropine-positive roots.

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Claims 11

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Exogenous polyamides with auxin promoted early olive rooting.

Taxon
olive Olea europaea (L.)Olea europaeae0
Treatment
polyamides (PAs), putrescine, spermidine, and sperminee0
Outcome
early rootinge0
Direction
promotede0
Setting
in vitro rooting mediume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In the olive Olea europaea (L.), the polyamides (PAs), putrescine, spermidine, and spermine, when added exogenously at a concentration of 1 m m in the in vitro rooting medium, combined with 5 μ m auxin concentration, promoted early rooting and increased the final rooting percentage and the number of roots per explant.”

✓ located verbatim in the source · abstract, characters 0–319 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Exogenous polyamides with auxin increased final olive rooting percentage and roots per explant.

Taxon
olive Olea europaea (L.)Olea europaeae0
Treatment
polyamides (PAs), putrescine, spermidine, and sperminee0
Outcome
final rooting percentage and the number of roots per explante0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In the olive Olea europaea (L.), the polyamides (PAs), putrescine, spermidine, and spermine, when added exogenously at a concentration of 1 m m in the in vitro rooting medium, combined with 5 μ m auxin concentration, promoted early rooting and increased the final rooting percentage and the number of roots per explant.”

✓ located verbatim in the source · abstract, characters 0–319 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

The polyamide effect was less evident in olive explants rooted by basal blanching.

Taxon
oliveOlea europaeae0
Treatment
basal blanching methode0
Outcome
effecte0
Direction
less evidente0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The effect was less evident in olive explants rooted with the basal blanching method”

✓ located verbatim in the source · abstract, characters 320–404 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Endogenous polyamide levels in blanched olive explants were lower on day 2 than controls.

Treatment
`these explantse0
Outcome
total endogenous PAse0
Direction
lowere0
Qualifier
day 2e0
Comparator
controlse0

Not reportedTaxon

Verbatim

e0

“thin-layer chromatography of total endogenous PAs in `these explants revealed lower levels on day 2 compared with controls”

✓ located verbatim in the source · abstract, characters 406–528 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Polyamide concentrations in treated and control explants fell to similar levels by day 5.

Outcome
PA concentrationse0
Direction
fallen to similar levelse0
Qualifier
by day 5e0

Not reportedTaxon

Verbatim

e0

“by day 5 PA concentrations in both had fallen to similar levels”

✓ located verbatim in the source · abstract, characters 536–599 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Putrescine lowered rooting-medium pH by 0.5 units around explants.

Treatment
putrescinee0
Outcome
pH of the mediume0
Direction
decreasede0
Value
0.5 unitse0

Not reportedTaxonComparator

Verbatim

e0

“putrescine decreased the pH of the medium by 0.5 units around the explants”

✓ located verbatim in the source · abstract, characters 614–688 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Polyamides had little effect on apple rooting.

Taxon
apple Malus pumila (Mill.)Malus pumilae0
Treatment
PAse0
Outcome
effecte0
Direction
little effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus pumila
Malus pumila · TaxonЛобачев Владимир · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“PAs had little effect on apple Malus pumila (Mill.)”

✓ located verbatim in the source · abstract, characters 690–741 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Polyamides had no effect on almond.

Taxon
almond Prunus dulcis (Miller) D.A. WebbPrunus dulcise0
Treatment
PAse0
Outcome
effecte0
Direction
no effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus dulcis
Prunus dulcis · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“PAs had little effect on apple Malus pumila (Mill.) and no effect on almond Prunus dulcis (Miller) D.A. Webb”

✓ located verbatim in the source · abstract, characters 690–798 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Polyamides had no effect on pistachio.

Taxon
pistachio Pistacia vera (L.)Pistacia verae0
Treatment
PAse0
Outcome
no effecte0
Direction
no effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pistacia vera
Pistacia vera · TaxonStan Shebs · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“PAs had little effect on apple Malus pumila (Mill.) and no effect on almond Prunus dulcis (Miller) D.A. Webb and pistachio Pistacia vera (L.)”

✓ located verbatim in the source · abstract, characters 690–831 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Agrobacterium rhizogenes induced some positive rooting effects only in olive in auxin-free medium.

Taxon
oliveOlea europaeae0
Treatment
Agrobacterium rhizogenese0
Interactor
Agrobacterium rhizogenese0other
Outcome
positive effectse0
Direction
positivee0
Setting
auxin-free mediume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“There were also some positive effects observed, but only in olive, when rooting was induced by Agrobacterium rhizogenes in auxin-free medium.”

✓ located verbatim in the source · abstract, characters 833–974 · show in text ↑

putrescine · growth · Olea europaea (common olive)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.