Abstract
Salicylic acid (SA) is a defense-related key signaling molecule involved in plant immunity. In this study, a subgroup IIa WRKY gene PtrWRKY40 was isolated from Populus trichocarpa, which displayed amino acid sequence similar to Arabidopsis AtWRKY40, AtWRKY18 and AtWRKY60. PtrWRKY40 transcripts accumulated significantly in response to SA, methyl jasmonate and hemibiotrophic fungus Dothiorella gregaria Sacc. Overexpression of PtrWRKY40 in transgenic poplar conferred higher susceptibility to D. gregaria infection. This susceptibility was coupled with reduced expression of SA-associated genes (PR1.1, PR2.1, PR5.9, CPR5 and SID2) and jasmonic acid (JA)-related gene JAZ8. Decreased accumulation of endogenous SA was observed in transgenic lines overexpressing PtrWRKY40 when compared with wild-type plants. However, constitutive expression of PtrWRKY40 in Arabidopsis thaliana displayed resistance to necrotrophic fungus Botrytis cinerea, and the expression of JA-defense-related genes such as PDF1.2, VSP2 and PR3 was remarkably increased in transgenic plants upon infection with fugal pathogens. Together, our findings indicate that PtrWRKY40 plays a negative role in resistance to hemibiotrophic fungi in poplar but functions as a positive regulator of resistance toward the necrotrophic fungi in Arabidopsis.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
PtrWRKY40 was isolated from Populus trichocarpa and had sequence similarity to Arabidopsis WRKY proteins.
- Taxon
- Populus trichocarpa
Populus trichocarpae0 - Gene
- PtrWRKY40e0
- Outcome
- amino acid sequence similar to Arabidopsis AtWRKY40, AtWRKY18 and AtWRKY60e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a subgroup IIa WRKY gene PtrWRKY40 was isolated from Populus trichocarpa, which displayed amino acid sequence similar to Arabidopsis AtWRKY40, AtWRKY18 and AtWRKY60”
Claim · model-written summary
PtrWRKY40 transcripts increased significantly in response to salicylic acid.
- Taxon
- Populus trichocarpaPopulus trichocarpat0
- Treatment
- SAe0
- Gene
- PtrWRKY40e0
- Outcome
- PtrWRKY40 transcriptse0
- Direction
- accumulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtrWRKY40 transcripts accumulated significantly in response to SA, methyl jasmonate and hemibiotrophic fungus Dothiorella gregaria Sacc.”
t0“Populus trichocarpa”
Claim · model-written summary
PtrWRKY40 transcripts increased significantly in response to methyl jasmonate.
- Taxon
- Populus trichocarpaPopulus trichocarpat0
- Treatment
- methyl jasmonatee0
- Gene
- PtrWRKY40e0
- Outcome
- PtrWRKY40 transcriptse0
- Direction
- accumulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtrWRKY40 transcripts accumulated significantly in response to SA, methyl jasmonate and hemibiotrophic fungus Dothiorella gregaria Sacc.”
t0“Populus trichocarpa”
Claim · model-written summary
PtrWRKY40 transcripts increased significantly in response to Dothiorella gregaria.
- Taxon
- Populus trichocarpaPopulus trichocarpat0
- Treatment
- hemibiotrophic fungus Dothiorella gregaria Sacc.e0
- Interactor
- Dothiorella gregaria Sacc.
Dothiorella gregariae0pathogen - Gene
- PtrWRKY40e0
- Outcome
- PtrWRKY40 transcriptse0
- Direction
- accumulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtrWRKY40 transcripts accumulated significantly in response to SA, methyl jasmonate and hemibiotrophic fungus Dothiorella gregaria Sacc.”
t0“Populus trichocarpa”
Claim · model-written summary
PtrWRKY40 overexpression increased poplar susceptibility to D. gregaria.
- Treatment
- Overexpression of PtrWRKY40e0
- Interactor
- D. gregariaDothiorella gregariae0pathogen
- Gene
- PtrWRKY40e0
- Outcome
- susceptibility to D. gregaria infectione0
- Direction
- highere0
PopulusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of PtrWRKY40 in transgenic poplar conferred higher susceptibility to D. gregaria infection”
Claim · model-written summary
PtrWRKY40 overexpression reduced expression of SA- and JA-associated defense genes in poplar.
- Gene
- PR1.1, PR2.1, PR5.9, CPR5 and SID2e0
- Outcome
- expression of SA-associated genese0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This susceptibility was coupled with reduced expression of SA-associated genes (PR1.1, PR2.1, PR5.9, CPR5 and SID2) and jasmonic acid (JA)-related gene JAZ8”
t0“transgenic poplar”
Claim · model-written summary
PtrWRKY40 overexpression decreased endogenous SA in transgenic poplar.
- Material
- transgenic linese0
- Gene
- PtrWRKY40e0
- Outcome
- accumulation of endogenous SAe0
- Direction
- Decreasede0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Decreased accumulation of endogenous SA was observed in transgenic lines overexpressing PtrWRKY40 when compared with wild-type plants”
t0“transgenic poplar”
Claim · model-written summary
Constitutive PtrWRKY40 expression increased Arabidopsis resistance to Botrytis cinerea.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- constitutive expression of PtrWRKY40e0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Gene
- PtrWRKY40e0
- Outcome
- resistance to necrotrophic fungus Botrytis cinereae0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“constitutive expression of PtrWRKY40 in Arabidopsis thaliana displayed resistance to necrotrophic fungus Botrytis cinerea”
Claim · model-written summary
JA-defense gene expression increased in transgenic Arabidopsis after fungal infection.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- PDF1.2, VSP2 and PR3e0
- Outcome
- expression of JA-defense-related genese0
- Direction
- remarkably increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of JA-defense-related genes such as PDF1.2, VSP2 and PR3 was remarkably increased in transgenic plants upon infection with fugal pathogens”
t0“Arabidopsis thaliana”
The same questions in other papers
(-)-methyl jasmonate · molecular: expression · Populus trichocarpa (black cottonwood)Results agree
1 paper in the same direction as this paper
- positive
overexpression · Botrytis cinerea · Arabidopsis thaliana (thale cress)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.