Abstract
Previous studies indicated that methyl jasmonate (MeJA) treatment could effectively reduce the chilling injury of many fruits, including banana, but the underlying mechanism is poorly understood. In this study, one lateral organ boundaries (LOB) domain (LBD) gene, designated as MaLBD5, was isolated and characterized from banana fruit. Expression analysis revealed that accumulation of MaLBD5 was induced by cold temperature and MeJA treatment. Subcellular localization and transactivation assays showed that MaLBD5 was localized to the nucleus and possessed transcriptional activation activity. Protein-protein interaction analysis demonstrated that MaLBD5 physically interacted with MaJAZ1, a potential repressor of jasmonate signaling. Furthermore, transient expression assays indicated that MaLBD5 transactivated a jasmonate biosynthesis gene, termed MaAOC2, which was also induced by cold and MeJA. More interestingly, MaJAZ1 attenuated the MaLBD5-mediated transactivation of MaAOC2. These results suggest that MaLBD5 and MaJAZ1 might act antagonistically in relation to MeJA-induced cold tolerance of banana fruit, at least partially via affecting jasmonate biosynthesis. Collectively, our findings expand the knowledge of the transcriptional regulatory network of MeJA-mediated cold tolerance of banana fruit.
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Claims 11
Claim · model-written summary
MaLBD5 was isolated and characterized from banana fruit.
Verbatim
e0“one lateral organ boundaries (LOB) domain (LBD) gene, designated as MaLBD5, was isolated and characterized from banana fruit”
Claim · model-written summary
Cold temperature induced MaLBD5 accumulation.
- Taxon
- banana fruitt0
- Treatment
- cold temperaturee0
- Gene
- MaLBD5e0
- Outcome
- accumulation of MaLBD5e0
- Direction
- inducede0
Not reportedComparator
Verbatim
e0“accumulation of MaLBD5 was induced by cold temperature and MeJA treatment”
t0“Cold Tolerance of Banana Fruit”
Claim · model-written summary
MeJA treatment induced MaLBD5 accumulation.
- Taxon
- banana fruitt0
- Treatment
- MeJA treatmente0
- Gene
- MaLBD5e0
- Outcome
- accumulation of MaLBD5e0
- Direction
- inducede0
Not reportedComparator
Verbatim
e0“accumulation of MaLBD5 was induced by cold temperature and MeJA treatment”
t0“Cold Tolerance of Banana Fruit”
Claim · model-written summary
MaLBD5 was localized to the nucleus.
Verbatim
e0“MaLBD5 was localized to the nucleus and possessed transcriptional activation activity”
t0“Cold Tolerance of Banana Fruit”
Claim · model-written summary
MaLBD5 had transcriptional activation activity.
Verbatim
e0“MaLBD5 was localized to the nucleus and possessed transcriptional activation activity”
t0“Cold Tolerance of Banana Fruit”
Claim · model-written summary
MaLBD5 physically interacted with MaJAZ1.
Verbatim
e0“MaLBD5 physically interacted with MaJAZ1, a potential repressor of jasmonate signaling”
t0“Cold Tolerance of Banana Fruit”
Claim · model-written summary
MaLBD5 transactivated MaAOC2 in transient expression assays.
Verbatim
e0“MaLBD5 transactivated a jasmonate biosynthesis gene, termed MaAOC2”
t0“Cold Tolerance of Banana Fruit”
Claim · model-written summary
Cold induced MaAOC2.
Not reportedComparator
Verbatim
e0“MaAOC2, which was also induced by cold and MeJA”
t0“Cold Tolerance of Banana Fruit”
Claim · model-written summary
MeJA induced MaAOC2.
Not reportedComparator
Verbatim
e0“MaAOC2, which was also induced by cold and MeJA”
t0“Cold Tolerance of Banana Fruit”
Claim · model-written summary
MaJAZ1 attenuated MaLBD5-mediated MaAOC2 transactivation.
- Taxon
- banana fruitt0
- Treatment
- MaJAZ1e0
- Gene
- MaJAZ1e0
- Outcome
- MaLBD5-mediated transactivation of MaAOC2e0
- Direction
- attenuatede0
Not reportedComparator
Verbatim
e0“MaJAZ1 attenuated the MaLBD5-mediated transactivation of MaAOC2”
t0“Cold Tolerance of Banana Fruit”
Claim · model-written summary
The authors suggest MaLBD5 and MaJAZ1 might act antagonistically in MeJA-induced cold tolerance, partly through jasmonate biosynthesis.
Verbatim
e0“MaLBD5 and MaJAZ1 might act antagonistically in relation to MeJA-induced cold tolerance of banana fruit, at least partially via affecting jasmonate biosynthesis”
The same questions in other papers
(-)-methyl jasmonate · molecular: metabolites · MusaResults agree
2 papers in the same direction as this paper
- positive
- positive
cold · molecular: metabolites · MusaPositive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.