Abstract
In vitro studies were undertaken for culture establishment in pomegranate cv. G-137 using shoot tips and nodal segment collected from mature plants. Sealing of the cut ends of explants with pre-sterilized paraffin was found to minimize browning by preventing phenol exudation and giving high culture establishment (83.95%) as compared to control (17.55%). Fungicidal pre-treatment of explants with Bavistin® 0.1% (carbendazim) + Ridomil® 0.1% (Syngenta) for 1 h gave the highest culture establishment (80.60%) with nodal segment and lowest microbial contamination (40.60%), with shoot tip as explant. Interaction between explant, sterilant and duration of treatment revealed that highest culture establishment (81.00%) was observed with 3 min. surface sterilization with 0.1% HgCl2. The maximum culture establishment was obtained with treatment 2.0 mg/l BAP + 0.1 mg/l NAA + 0.5 mg/l GA which also gave the earliest bud sprouting (5.67 days) as compared to control.
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Claims 7
Claim · model-written summary
Paraffin sealing minimized explant browning.
- Taxon
- pomegranate
Punica granatumt0 - Treatment
- pre-sterilized paraffine0
- Outcome
- browninge0
- Direction
- minimizee0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sealing of the cut ends of explants with pre-sterilized paraffin was found to minimize browning by preventing phenol exudation and giving high culture establishment (83.95%) as compared to control (17.55%)”
t0“pomegranate”
Claim · model-written summary
Paraffin sealing gave 83.95% culture establishment versus 17.55% for the control.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- pre-sterilized paraffine0
- Outcome
- culture establishmente0
- Direction
- highe0
- Value
- 83.95%e0
- Qualifier
- 17.55%e0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sealing of the cut ends of explants with pre-sterilized paraffin was found to minimize browning by preventing phenol exudation and giving high culture establishment (83.95%) as compared to control (17.55%)”
t0“pomegranate”
Claim · model-written summary
Fungicide pretreatment gave 80.60% establishment with nodal segments.
- Taxon
- pomegranatePunica granatumt0
- Organ / stage
- nodal segmente0
- Treatment
- Bavistin® 0.1% (carbendazim) + Ridomil® 0.1% (Syngenta) for 1 he0
- Outcome
- culture establishmente0
- Direction
- higheste0
- Value
- 80.60%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fungicidal pre-treatment of explants with Bavistin® 0.1% (carbendazim) + Ridomil® 0.1% (Syngenta) for 1 h gave the highest culture establishment (80.60%) with nodal segment”
t0“pomegranate”
Claim · model-written summary
Fungicide pretreatment gave the lowest microbial contamination, 40.60%, in shoot tips.
- Taxon
- pomegranatePunica granatumt0
- Organ / stage
- shoot tipe0
- Outcome
- microbial contaminatione0
- Direction
- loweste0
- Value
- 40.60%e0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lowest microbial contamination (40.60%), with shoot tip as explant”
t0“pomegranate”
Claim · model-written summary
Three minute HgCl2 sterilization gave 81.00% culture establishment.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- 3 min. surface sterilization with 0.1% HgCl2e0
- Outcome
- culture establishmente0
- Direction
- higheste0
- Value
- 81.00%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“highest culture establishment (81.00%) was observed with 3 min. surface sterilization with 0.1% HgCl2”
t0“pomegranate”
Claim · model-written summary
BAP, NAA and GA treatment maximized culture establishment.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- 2.0 mg/l BAP + 0.1 mg/l NAA + 0.5 mg/l GAe0
- Outcome
- culture establishmente0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum culture establishment was obtained with treatment 2.0 mg/l BAP + 0.1 mg/l NAA + 0.5 mg/l GA”
t0“pomegranate”
Claim · model-written summary
The same hormone treatment produced earliest bud sprouting at 5.67 days versus control.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- 2.0 mg/l BAP + 0.1 mg/l NAA + 0.5 mg/l GAe0
- Outcome
- bud sproutinge0
- Direction
- earlieste0
- Value
- 5.67 dayse0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment 2.0 mg/l BAP + 0.1 mg/l NAA + 0.5 mg/l GA which also gave the earliest bud sprouting (5.67 days) as compared to control”
t0“pomegranate”
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See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.