Paper · 2013 · Journal of Applied Horticulture

Improvement of somatic embryogenesis and plant regeneration of seven date palm (Phoenix dactylifera L.) cultivars: Effect of cytokinins and activated charcoal

Mona M. Hassan; Ibrahim A. Ibrahim; Mohsen K.H. Ebrahim; et al.

7
claims
15
verbatim quotes
4
citations
3
research areas
—
Not open access

Abstract

This study presents a procedure for the rapid development of a large number of somatic embryos and shoots from seven date palm cultivars (Barthamuda, Sakkoty, Malkaby, Shamia, Khalas, Barhee and Medjool). Clusters of leaf meristem explants were cultured on Murashige and Skoog medium supplemented with 0.1 mg/L naphthalene acetic acid and cytokinins (benzyl adenine, kinetin or 2-isopentenyl adenine) at 0.05 or 0.1 mg/L, in the presence or absence of activated charcoal. Regeneration to newly formed embryos and shoot formation was significantly (P<0.05) promoted in all cultivars using a culture medium with 0.05 mg/L benzyl adenine in the absence of activated charcoal. The presence of charcoal was inhibitory to shoot formation in all cases, except on the medium with 0.05 mg/L benzyl adenine, where it stimulated 33% shoot formation. This medium plus activated charcoal is therefore the recommended one for shoot formation. The number of somatic embryos and shoots generated was greatest with cultivars Barthamuda, Barhee and Sakkoty, while Medjool had the lowest number. The healthy shoots were suitable for acclimatization to form plantlets in soil.

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Claims 7

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Medium with 0.05 mg/L benzyl adenine without activated charcoal significantly promoted embryos and shoots in all cultivars.

Taxon
date palmPhoenix dactyliferat0
Treatment
0.05 mg/L benzyl adenine in the absence of activated charcoale0
Outcome
newly formed embryos and shoot formatione0
Direction
promotede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Regeneration to newly formed embryos and shoot formation was significantly (P<0.05) promoted in all cultivars using a culture medium with 0.05 mg/L benzyl adenine in the absence of activated charcoal.”

✓ located verbatim in the source · abstract, characters 472–672 · show in text ↑
t0

“date palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 69–103 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Charcoal inhibited shoot formation except with 0.05 mg/L benzyl adenine.

Taxon
date palmPhoenix dactyliferat0
Treatment
charcoale0
Outcome
shoot formatione0
Direction
inhibitorye0
Qualifier
except on the medium with 0.05 mg/L benzyl adeninee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The presence of charcoal was inhibitory to shoot formation in all cases, except on the medium with 0.05 mg/L benzyl adenine, where it stimulated 33% shoot formation.”

✓ located verbatim in the source · abstract, characters 673–838 · show in text ↑
t0

“date palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 69–103 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

With 0.05 mg/L benzyl adenine, charcoal stimulated 33% shoot formation.

Taxon
date palmPhoenix dactyliferat0
Treatment
0.05 mg/L benzyl adeninee0
Outcome
shoot formatione0
Direction
stimulatede0
Value
33%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“on the medium with 0.05 mg/L benzyl adenine, where it stimulated 33% shoot formation”

✓ located verbatim in the source · abstract, characters 753–837 · show in text ↑
t0

“date palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 69–103 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors recommend medium with benzyl adenine and activated charcoal for shoot formation.

Taxon
date palmPhoenix dactyliferat0
Treatment
activated charcoale0
Outcome
recommended one for shoot formatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“This medium plus activated charcoal is therefore the recommended one for shoot formation.”

✓ located verbatim in the source · abstract, characters 839–928 · show in text ↑
e1

“medium with 0.05 mg/L benzyl adenine in the absence of activated charcoal”

✓ located verbatim in the source · abstract, characters 598–671 · show in text ↑
t0

“date palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 69–103 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Barthamuda, Barhee and Sakkoty generated the greatest numbers of somatic embryos and shoots.

Taxon
date palmPhoenix dactyliferat0
Material
Barthamuda, Barhee and Sakkotye0
Outcome
number of somatic embryos and shootse0
Direction
greateste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The number of somatic embryos and shoots generated was greatest with cultivars Barthamuda, Barhee and Sakkoty, while Medjool had the lowest number.”

✓ located verbatim in the source · abstract, characters 929–1076 · show in text ↑
t0

“date palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 69–103 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Medjool generated the lowest numbers of somatic embryos and shoots.

Taxon
date palmPhoenix dactyliferat0
Material
Medjoole0
Outcome
number of somatic embryos and shootse0
Direction
loweste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The number of somatic embryos and shoots generated was greatest with cultivars Barthamuda, Barhee and Sakkoty, while Medjool had the lowest number.”

✓ located verbatim in the source · abstract, characters 929–1076 · show in text ↑
t0

“date palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 69–103 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Healthy shoots were suitable for acclimatization into soil plantlets.

Taxon
date palmPhoenix dactyliferat0
Organ / stage
shootse0
Outcome
suitable for acclimatization to form plantlets in soile0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The healthy shoots were suitable for acclimatization to form plantlets in soil.”

✓ located verbatim in the source · abstract, characters 1077–1156 · show in text ↑
t0

“date palm (Phoenix dactylifera L.)”

✓ located verbatim in the source · title, characters 69–103 · show in text ↑

charcoal · growth · Phoenix dactylifera (date palm)Results differ

16 papers: 15 positive, 1 negative or no effect

14 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. no effect
  3. positive

12 papers more in this group

See all 16 papers →

N-benzyladenine · growth · Phoenix dactylifera (date palm)Positive and negative results

23 papers: 21 positive, 2 negative or no effect

20 papers in the same direction as this paper · 1 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
  3. positive

19 papers more in this group

See all 23 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.