Paper · 2017 · Global Journal Of Botanical Science

Micropropagation of some Sewa Oasis Date Palm (Phoenix dactylifera L.) Cultivars Grown in Egypt

Ibrahim A. Ibrahim; Mahdia F. Gabr; Mahmoud I. Nasr; et al.

12
claims
22
verbatim quotes
0
citations
3
research areas
✓
Open access · cc-by-nc

Abstract

Shoot tip and leaf primordial explants of Phoenix dactylifera L. cvs. (Sewi and Oshkingbeel) were cultured in modified MS medium supplemented with various combinations between different growth regulators cytokinins (2iP or BA) and Auxins (2, 4-D or NAA). Friable callus was produced from shoot tip and leaf primordial explants. The highest percentage of callus initiation was achieved on modified MS medium supplemented with 100 mg/L 2,4-D and 3 mg/L 2iP for shoot tip and leaf primordial explants. The highest percentage of embryogenic callus was achieved on modified MS medium supplemented with 10 mg/L 2,4-D, 5 mg/L NAA, 3 mg/L 2iP and 3.0 g/L activated charcoal which gave 87.5% and 75% for Sewi and Oshkingbeel cvs., respectively. Somatic embryos formation were obvious on modified MS medium plus 0.1 mg/L NAA which gave the highest average number (5.16, 4.94) for Sewi and Oshkingbeel, respectively after three subcultures. Greatest embryonic elongation resulted at 0.5 mg/L 2iP + 0.5 mg/L kinetin + 40 g/L sucrose in solid full strength MS medium. Also, the highest value of leaves number was shown on the same medium. The complete plantlets developed from individual embryos (5-10 cm) were transferred to rooting MS medium supplemented with 1 mg/L NAA with good growth in solid and liquid media. Rooted date palm plantlets from both cultivars were transferred to plastic pots (5cm diameter) containing peat-moss, sand and vermiculite at equal volume. Acclimatization percentage decreased gradually during four months in the first acclimatization stage. Date palm plantlets produced from the first acclimatization stage were transferred to plastic pots (20 cm diameter) containing peatmoss, washed sand and vermiculite at equal volume for more growth and development to be ready to cultivate in the field. Although survival percentage was low during the first acclimatization stage, it was very high in the second acclimatization stage and date palm survival percentage became constant after another six months with good growth.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 12

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Friable callus formed from shoot tip and leaf primordial explants.

Taxon
Phoenix dactylifera L.Phoenix dactyliferat0
Organ / stage
shoot tip and leaf primordial explantse0
Outcome
Friable callus was producede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Friable callus was produced from shoot tip and leaf primordial explants”

✓ located verbatim in the source · abstract, characters 255–326 · show in text ↑
t0

“Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 47–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The medium with 100 mg/L 2,4-D and 3 mg/L 2iP gave the highest callus initiation percentage.

Taxon
Phoenix dactylifera L.Phoenix dactyliferat0
Organ / stage
shoot tip and leaf primordial explantse0
Treatment
modified MS medium supplemented with 100 mg/L 2,4-D and 3 mg/L 2iPe0
Outcome
percentage of callus initiatione0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest percentage of callus initiation was achieved on modified MS medium supplemented with 100 mg/L 2,4-D and 3 mg/L 2iP for shoot tip and leaf primordial explants”

✓ located verbatim in the source · abstract, characters 328–497 · show in text ↑
t0

“Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 47–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sewi achieved 87.5% embryogenic callus with the stated medium.

Taxon
Phoenix dactylifera L.Phoenix dactyliferat0
Material
Sewie0
Treatment
modified MS medium supplemented with 10 mg/L 2,4-D, 5 mg/L NAA, 3 mg/L 2iP and 3.0 g/L activated charcoale0
Outcome
percentage of embryogenic calluse0
Direction
higheste0
Value
87.5%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest percentage of embryogenic callus was achieved on modified MS medium supplemented with 10 mg/L 2,4-D, 5 mg/L NAA, 3 mg/L 2iP and 3.0 g/L activated charcoal which gave 87.5% and 75% for Sewi and Oshkingbeel cvs., respectively”

✓ located verbatim in the source · abstract, characters 499–734 · show in text ↑
t0

“Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 47–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Oshkingbeel achieved 75% embryogenic callus with the stated medium.

Taxon
Phoenix dactylifera L.Phoenix dactyliferat0
Material
Oshkingbeele0
Treatment
modified MS medium supplemented with 10 mg/L 2,4-D, 5 mg/L NAA, 3 mg/L 2iP and 3.0 g/L activated charcoale0
Outcome
percentage of embryogenic calluse0
Direction
higheste0
Value
75%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest percentage of embryogenic callus was achieved on modified MS medium supplemented with 10 mg/L 2,4-D, 5 mg/L NAA, 3 mg/L 2iP and 3.0 g/L activated charcoal which gave 87.5% and 75% for Sewi and Oshkingbeel cvs., respectively”

✓ located verbatim in the source · abstract, characters 499–734 · show in text ↑
t0

“Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 47–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sewi produced an average of 5.16 somatic embryos on 0.1 mg/L NAA medium after three subcultures.

Taxon
Phoenix dactylifera L.Phoenix dactyliferat0
Material
Sewie0
Treatment
modified MS medium plus 0.1 mg/L NAAe0
Outcome
Somatic embryos formatione0
Direction
higheste0
Value
5.16e0
Qualifier
after three subculturese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Somatic embryos formation were obvious on modified MS medium plus 0.1 mg/L NAA which gave the highest average number (5.16, 4.94) for Sewi and Oshkingbeel, respectively after three subcultures”

✓ located verbatim in the source · abstract, characters 736–928 · show in text ↑
t0

“Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 47–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Oshkingbeel produced an average of 4.94 somatic embryos on 0.1 mg/L NAA medium after three subcultures.

Taxon
Phoenix dactylifera L.Phoenix dactyliferat0
Material
Oshkingbeele0
Treatment
modified MS medium plus 0.1 mg/L NAAe0
Outcome
Somatic embryos formatione0
Direction
higheste0
Value
4.94e0
Qualifier
after three subculturese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Somatic embryos formation were obvious on modified MS medium plus 0.1 mg/L NAA which gave the highest average number (5.16, 4.94) for Sewi and Oshkingbeel, respectively after three subcultures”

✓ located verbatim in the source · abstract, characters 736–928 · show in text ↑
t0

“Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 47–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The specified solid medium yielded greatest embryonic elongation and highest leaf number.

Taxon
Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
0.5 mg/L 2iP + 0.5 mg/L kinetin + 40 g/L sucrose in solid full strength MS mediume0
Outcome
embryonic elongatione0
Direction
Greateste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Greatest embryonic elongation resulted at 0.5 mg/L 2iP + 0.5 mg/L kinetin + 40 g/L sucrose in solid full strength MS medium. Also, the highest value of leaves number was shown on the same medium”

✓ located verbatim in the source · abstract, characters 930–1124 · show in text ↑
t0

“Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 47–69 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Plantlets developed and grew well in rooting MS medium with 1 mg/L NAA.

Taxon
Phoenix dactylifera L.Phoenix dactyliferat0
Treatment
rooting MS medium supplemented with 1 mg/L NAAe0
Outcome
good growthe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The complete plantlets developed from individual embryos (5-10 cm) were transferred to rooting MS medium supplemented with 1 mg/L NAA with good growth in solid and liquid media”

✓ located verbatim in the source · abstract, characters 1126–1302 · show in text ↑
t0

“Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 47–69 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Acclimatization percentage declined over four months in the first stage.

Taxon
Phoenix dactylifera L.Phoenix dactyliferat0
Outcome
Acclimatization percentagee0
Direction
decreasede0
Qualifier
during four months in the first acclimatization stagee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Acclimatization percentage decreased gradually during four months in the first acclimatization stage”

✓ located verbatim in the source · abstract, characters 1459–1559 · show in text ↑
t0

“Phoenix dactylifera L.”

✓ located verbatim in the source · title, characters 47–69 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Date palm survival was low in the first acclimatization stage.

Taxon
date palmPhoenix dactyliferat0
Outcome
survival percentagee0
Direction
lowe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“survival percentage was low during the first acclimatization stage”

✓ located verbatim in the source · abstract, characters 1822–1888 · show in text ↑
t0

“Date Palm”

✓ located verbatim in the source · title, characters 36–45 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Date palm survival was very high in the second acclimatization stage.

Taxon
date palmPhoenix dactyliferae0
Outcome
date palm survival percentagee0
Direction
very highe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“it was very high in the second acclimatization stage and date palm survival percentage became constant after another six months with good growth”

✓ located verbatim in the source · abstract, characters 1890–2034 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Date palm survival stabilized after another six months with good growth.

Taxon
date palmPhoenix dactyliferae0
Outcome
date palm survival percentagee0
Direction
became constante0
Qualifier
after another six monthse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“date palm survival percentage became constant after another six months with good growth”

✓ located verbatim in the source · abstract, characters 1947–2034 · show in text ↑

charcoal · growth · Phoenix dactylifera (date palm)Results differ

16 papers: 15 positive, 1 negative or no effect

14 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. no effect
  3. positive

12 papers more in this group

See all 16 papers →

kinetin · growth · Phoenix dactylifera (date palm)Positive and negative results

6 papers: 4 positive, 2 negative or no effect

3 papers in the same direction as this paper · 1 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
  3. positive

2 papers more in this group

See all 6 papers →

2,4-D · growth · Phoenix dactylifera (date palm)Positive and negative results

25 papers: 22 positive, 3 negative or no effect

21 papers in the same direction as this paper · 3 in the opposite direction

  1. negative
  2. no effect
  3. negative

21 papers more in this group

See all 25 papers →

1-naphthaleneacetic acid · growth · Phoenix dactylifera (date palm)Positive and negative results

39 papers: 38 positive, 1 negative or no effect

37 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

35 papers more in this group

See all 39 papers →

sucrose · growth · Phoenix dactylifera (date palm)Results agree

20 papers: 20 positive, 0 negative or no effect

19 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

16 papers more in this group

See all 20 papers →

in vitro · growth · Phoenix dactylifera (date palm)Positive and negative results

51 papers: 50 positive, 1 negative or no effect

49 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

47 papers more in this group

See all 51 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.