Abstract
BACKGROUND: Zinc finger proteins (ZFPs) play an important role in regulating plant responses to abiotic stress. However, little is known about the function of LSD1-like-type ZFP in saline-alkaline (SA) stress resistance of rice. In this study, OsLOL5 (GenBank No. AJ620677), containing two LSD1-like-type C2C2 domains, was isolated and analyzed its protection roles in transgenic plants and yeast. OsLOL5 was located in the nucleus as evidenced by the bombardment of onion epidermal cells. RESULTS: OsLOL5 expression significantly increased in rice leaves and roots under 150 mmol L-1 NaCl, 30 mM NaHCO3, and 10 mmol L-1 H2O2 treatment, respectively. Overexpression of OsLOL5 in yeast resulted in SA tolerance at significant level. Transgenic Arabidopsis plants overexpressing OsLOL5 grew well in the presence of both NaCl and NaHCO3 treatments, whereas wild-type plants exhibited chlorosis, stunted growth phenotype, and even death. SA stress caused significant changes in the malondialdehyde (MDA) contents in non-transgenic plants compared with those in transgenic lines. Transgenic rice overexpressing OsLOL5 exhibited stronger resistance than NT under NaHCO3 treatment, as demonstrated by its greater shoot length, and fresh weight. The genes associated with oxidative stress, such as OsAPX2, OsCAT, OsCu/Zn-SOD, and OsRGRC2, were significantly upregulated in OsLOL5-overexpressing rice. The results suggested that OsLOL5 improved SA tolerance in plants, and regulated oxidative and salinity stress retardation via the active oxygen detoxification pathway. CONCLUSIONS: The yeast INVScI bacterium grew significantly better than the control strain under NaCl, NaHCO3, and H2O2 treatments. These findings illustrated that OsLOL5 overexpression enhanced yeast resistance for SA stress through active oxygen species. The present study showed that the OsLOL5 genes involved in the ROS signaling pathways may combine with the model plant Arabidopsis and rice in LDS1-type ZFP by ROS signaling pathways that regulate cell necrosis. We speculated that the OsLOL5 active oxygen scavenging system may have coordinating roles. The present study further revealed that OsLOL5 ZFP could regulate oxidative stress function, but could also provide a basis for salt-resistant rice strains.
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Claims 11
Claim · model-written summary
OsLOL5 was located in the nucleus of onion epidermal cells.
- Taxon
- onion
Allium cepae0 - Organ / stage
- epidermal cellse0
- Gene
- OsLOL5e0
- Outcome
- located in the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsLOL5 was located in the nucleus as evidenced by the bombardment of onion epidermal cells.”
Claim · model-written summary
OsLOL5 expression increased significantly in rice leaves and roots under the listed NaCl, NaHCO3, and H2O2 treatments.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- leaves and rootse0
- Treatment
- 150 mmol L-1 NaCl, 30 mM NaHCO3, and 10 mmol L-1 H2O2 treatmente0
- Gene
- OsLOL5e0
- Outcome
- OsLOL5 expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsLOL5 expression significantly increased in rice leaves and roots under 150 mmol L-1 NaCl, 30 mM NaHCO3, and 10 mmol L-1 H2O2 treatment, respectively.”
Claim · model-written summary
OsLOL5 overexpression increased saline-alkaline tolerance in yeast.
- Taxon
- riceOryza sativat0
- Treatment
- Overexpression of OsLOL5e0
- Gene
- OsLOL5e0
- Outcome
- SA tolerancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of OsLOL5 in yeast resulted in SA tolerance at significant level.”
t0“rice LSD1-like-type ZFP gene OsLOL5 enhances saline-alkaline tolerance in transgenic Arabidopsis thaliana, yeast and rice”
Claim · model-written summary
Arabidopsis overexpressing OsLOL5 grew well under NaCl and NaHCO3 whereas wild type showed chlorosis, stunting and death.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- NaCl and NaHCO3 treatmentse0
- Gene
- OsLOL5e0
- Outcome
- grew welle0
- Comparator
- wild-type plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic Arabidopsis plants overexpressing OsLOL5 grew well in the presence of both NaCl and NaHCO3 treatments, whereas wild-type plants exhibited chlorosis, stunted growth phenotype, and even death.”
Claim · model-written summary
SA stress caused significant differences in malondialdehyde content between non-transgenic plants and transgenic lines.
- Taxon
- riceOryza sativat0
- Treatment
- SA stresse0
- Outcome
- malondialdehyde (MDA) contentse0
- Comparator
- transgenic linese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA stress caused significant changes in the malondialdehyde (MDA) contents in non-transgenic plants compared with those in transgenic lines.”
t0“rice LSD1-like-type ZFP gene OsLOL5 enhances saline-alkaline tolerance in transgenic Arabidopsis thaliana, yeast and rice”
Claim · model-written summary
OsLOL5-overexpressing rice had stronger resistance under NaHCO3 than non-transgenic rice.
- Taxon
- riceOryza sativae0
- Treatment
- NaHCO3 treatmente0
- Gene
- OsLOL5e0
- Outcome
- resistancee0
- Direction
- strongere0
- Comparator
- NTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice overexpressing OsLOL5 exhibited stronger resistance than NT under NaHCO3 treatment, as demonstrated by its greater shoot length, and fresh weight.”
Claim · model-written summary
OsLOL5-overexpressing rice had greater shoot length and fresh weight under NaHCO3 than non-transgenic rice.
- Taxon
- riceOryza sativae0
- Treatment
- NaHCO3 treatmente0
- Gene
- OsLOL5e0
- Outcome
- shoot length, and fresh weighte0
- Direction
- greatere0
- Comparator
- NTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice overexpressing OsLOL5 exhibited stronger resistance than NT under NaHCO3 treatment, as demonstrated by its greater shoot length, and fresh weight.”
Claim · model-written summary
OsAPX2, OsCAT, and OsCu/Zn-SOD were significantly upregulated in OsLOL5-overexpressing rice.
- Taxon
- riceOryza sativae0
- Gene
- OsAPX2, OsCAT, OsCu/Zn-SODe0
- Outcome
- significantly upregulatede0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genes associated with oxidative stress, such as OsAPX2, OsCAT, OsCu/Zn-SOD, and OsRGRC2, were significantly upregulated in OsLOL5-overexpressing rice.”
Claim · model-written summary
OsRGRC2 was significantly upregulated in OsLOL5-overexpressing rice.
- Taxon
- riceOryza sativae0
- Gene
- OsRGRC2e0
- Outcome
- significantly upregulatede0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genes associated with oxidative stress, such as OsAPX2, OsCAT, OsCu/Zn-SOD, and OsRGRC2, were significantly upregulated in OsLOL5-overexpressing rice.”
Claim · model-written summary
The authors infer that OsLOL5 improves saline-alkaline tolerance through active oxygen detoxification.
- Taxon
- riceOryza sativat0
- Gene
- OsLOL5e0
- Outcome
- SA tolerancee0
- Qualifier
- active oxygen detoxification pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results suggested that OsLOL5 improved SA tolerance in plants, and regulated oxidative and salinity stress retardation via the active oxygen detoxification pathway.”
t0“rice LSD1-like-type ZFP gene OsLOL5 enhances saline-alkaline tolerance in transgenic Arabidopsis thaliana, yeast and rice”
Claim · model-written summary
The yeast INVScI strain grew better than control under NaCl, NaHCO3 and H2O2.
- Taxon
- riceOryza sativat0
- Treatment
- NaCl, NaHCO3, and H2O2 treatmentse0
- Outcome
- yeast INVScI bacterium grewe0
- Direction
- bettere0
- Comparator
- control straine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The yeast INVScI bacterium grew significantly better than the control strain under NaCl, NaHCO3, and H2O2 treatments.”
t0“rice LSD1-like-type ZFP gene OsLOL5 enhances saline-alkaline tolerance in transgenic Arabidopsis thaliana, yeast and rice”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.