Abstract
Fragan × ananassa Duch.‘Toyonoka’plants were exposed to 5,10 and 15 mg · L-1 cadmium(CdCl2 · 2.5H2O)for 6 d to investigate the influence of heavy-metal Cd on root tips,which included DNA polymorphism change and oxidation systems damage.In addition,above indexes were analyzed by physiology biochemistry methods and random amplified polymorphic DNA(RAPD) technology.Cd induced an inhibited effect on root growth and soluble protein content,which also accumulated reactive oxygen species outbreak including superoxide anion production rate increased and hydrogen peroxide content enhanced.At the same time,antioxidant enzymes(SOD,POD and CAT) activities also decreased.Moreover,DNA hyperchromic effect reduced after 6 d on 5,10 or 15 mg · L-1 Cd treatment.Finally,RAPD results showed that 8 primers gave a total of 88 RAPD bands ranging from 150 to 3 500 bp in molecular size in the normal plantlets.The changes occurring in RAPD profiles of the root tips after Cd treatment,which included loss of normal bands,appearance of new bands and variation in band intensity in comparison to which of the plantlets without treatment.It is need to point out that the RAPD band change effect was dose-dependent of Cd concentration.These results indicated that genomic template stability(a qualitative measure reflecting changes in RAPD profiles)was significantly affected by the above Cd concentrations.Reactive oxygen species outbreak and DNA cross-linking were the main reasons which causing the root tips damage.Thus,DNA polymorphisms detected by RAPD analysis could be used as an effective method for the assessment of the root tips damage by Cd in strawberry plantlets.
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Claims 10
Claim · model-written summary
Cadmium inhibited strawberry root growth and soluble protein content.
- Taxon
- strawberry plants
_single2.jpg?width=60)
Fragaria ×ananassat0 - Treatment
- Cde0
- Outcome
- root growth and soluble protein contente0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Cd induced an inhibited effect on root growth and soluble protein content”
t0“strawberry plants”
Claim · model-written summary
Cadmium increased superoxide anion production and hydrogen peroxide content.
- Taxon
- strawberry plantsFragaria ×ananassat0
- Treatment
- Cde1
- Outcome
- superoxide anion production rate increased and hydrogen peroxide content enhancede0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“superoxide anion production rate increased and hydrogen peroxide content enhanced”
e1“Cd induced an inhibited effect”
t0“strawberry plants”
Claim · model-written summary
Cadmium decreased SOD, POD and CAT activities.
- Taxon
- strawberry plantsFragaria ×ananassat0
- Treatment
- Cd treatmente1
- Outcome
- antioxidant enzymes(SOD,POD and CAT) activitiese0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“antioxidant enzymes(SOD,POD and CAT) activities also decreased”
e1“root tips after Cd treatment”
t0“strawberry plants”
Claim · model-written summary
DNA hyperchromic effect decreased after cadmium treatment.
- Taxon
- strawberry plantsFragaria ×ananassat0
- Treatment
- 5,10 or 15 mg · L-1 Cd treatmente0
- Outcome
- DNA hyperchromic effecte0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA hyperchromic effect reduced after 6 d on 5,10 or 15 mg · L-1 Cd treatment”
t0“strawberry plants”
Claim · model-written summary
Eight primers yielded 88 RAPD bands in untreated plantlets.
Verbatim
e0“8 primers gave a total of 88 RAPD bands ranging from 150 to 3 500 bp in molecular size in the normal plantlets”
Claim · model-written summary
Cadmium altered root-tip RAPD profiles, with band loss, new bands and intensity variation relative to untreated plantlets.
Verbatim
e0“The changes occurring in RAPD profiles of the root tips after Cd treatment,which included loss of normal bands,appearance of new bands and variation in band intensity in comparison to which of the plantlets without treatment”
Claim · model-written summary
RAPD band changes depended on cadmium dose.
- Taxon
- strawberry plantsFragaria ×ananassat0
- Treatment
- Cd concentratione0
- Outcome
- RAPD band change effecte0
- Direction
- dose-dependente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RAPD band change effect was dose-dependent of Cd concentration”
t0“strawberry plants”
Claim · model-written summary
The tested cadmium concentrations significantly affected genomic template stability.
- Taxon
- strawberry plantsFragaria ×ananassat0
- Treatment
- Cd concentrationse0
- Outcome
- genomic template stabilitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genomic template stability(a qualitative measure reflecting changes in RAPD profiles)was significantly affected by the above Cd concentrations”
t0“strawberry plants”
Claim · model-written summary
Reactive oxygen species production and DNA cross-linking were presented as main causes of root-tip damage.
- Taxon
- strawberry plantsFragaria ×ananassat0
- Outcome
- root tips damagee0
- Qualifier
- Reactive oxygen species outbreak and DNA cross-linkinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reactive oxygen species outbreak and DNA cross-linking were the main reasons which causing the root tips damage”
t0“strawberry plants”
Claim · model-written summary
The authors propose RAPD-detected DNA polymorphisms for assessing cadmium damage in strawberry root tips.
- Taxon
- strawberry plantletsFragaria ×ananassae0
- Outcome
- assessment of the root tips damage by Cde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA polymorphisms detected by RAPD analysis could be used as an effective method for the assessment of the root tips damage by Cd in strawberry plantlets”
The same questions in other papers
cadmium atom · physiology: antioxidant enzymes · Fragaria x ananassa (strawberry)Results agree
2 papers in the same direction as this paper
- negative
- negative
cadmium atom · physiology: oxidative · Fragaria x ananassa (strawberry)Results agree
4 papers in the same direction as this paper
- negative
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.