Abstract
In order to understand the role of abscisic acid(ABA)in sweet cherry fruit maturation,one cDNA(PacNCED1)encoding 9-cis-epoxycarotenoid dioxygenase(NCED)as a key enzyme in ABA biosynthesis was cloned from the sweet cherry fruit,and one cDNA(PacACO1) encoding 1-aminocyclopropane-1-carboxylic acid(ACC)oxidase involved in ethylene biosynthesis was cloned from ethephon treated fruits using RT-PCR and RACE-PCR approach.The amino acid sequences of PacNCED1 and PacACO1 showed high homology to those of other NCEDs and ACOs.The expression patterns of PacNCED1 and PacACO1 were determined using RT-PCR and Real time PCR.The results indicated that the PacNCED1 was expressed continuously during the whole period of sweet cherry fruit development and in different tissues.Expression of PacNCED1 gene increased in the flesh and seeds and reached the maximum before maturation and that in pedicle reached the maximum at full maturity of sweet cherry fruit.PacNCED1 gene was weakly expressed in control leaves and roots,but sharply increased in water stressed leaves.Compared to the control,ABA and ethephon treatments could enhance the expression of PacNCED1 in fruit,while the NDGA and IAA treatments had no effect.The expression of PacACO1 during sweet cherry fruit development was not detected,but it could be induced with the ethephon and ABA treatments.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher.
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
PacNCED1 and PacACO1 cDNAs were cloned from sweet cherry fruit.
- Taxon
- sweet cherry
Prunus aviume0 - Gene
- PacNCED1e0
- Outcome
- cDNAe0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“one cDNA(PacNCED1)encoding 9-cis-epoxycarotenoid dioxygenase(NCED)as a key enzyme in ABA biosynthesis was cloned from the sweet cherry fruit,and one cDNA(PacACO1) encoding 1-aminocyclopropane-1-carboxylic acid(ACC)oxidase involved in ethylene biosynthesis was cloned from ethephon treated fruits”
Claim · model-written summary
PacNCED1 and PacACO1 amino acid sequences had high homology to other NCEDs and ACOs.
- Taxon
- Sweet CherryPrunus aviumt0
- Gene
- PacNCED1 and PacACO1e0
- Outcome
- high homologye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The amino acid sequences of PacNCED1 and PacACO1 showed high homology to those of other NCEDs and ACOs”
t0“Sweet Cherry Fruit”
Claim · model-written summary
PacNCED1 was expressed throughout sweet cherry fruit development and in different tissues.
- Taxon
- sweet cherryPrunus aviume0
- Organ / stage
- fruite0
- Gene
- PacNCED1e0
- Outcome
- expressed continuouslye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PacNCED1 was expressed continuously during the whole period of sweet cherry fruit development and in different tissues”
Claim · model-written summary
PacNCED1 expression rose in flesh and seeds, peaking before maturation, and peaked in pedicle at full maturity.
- Taxon
- sweet cherryPrunus aviume0
- Organ / stage
- flesh and seedse0
- Gene
- PacNCED1e0
- Outcome
- Expressione0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of PacNCED1 gene increased in the flesh and seeds and reached the maximum before maturation and that in pedicle reached the maximum at full maturity of sweet cherry fruit”
Claim · model-written summary
Water stress sharply increased PacNCED1 expression in leaves relative to weak expression in control leaves and roots.
- Taxon
- Sweet CherryPrunus aviumt0
- Treatment
- water stressede0
- Gene
- PacNCED1e0
- Outcome
- expressede0
- Direction
- sharply increasede0
- Comparator
- control leaves and rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PacNCED1 gene was weakly expressed in control leaves and roots,but sharply increased in water stressed leaves”
t0“Sweet Cherry Fruit”
Claim · model-written summary
ABA and ethephon enhanced PacNCED1 expression in fruit relative to control.
- Taxon
- Sweet CherryPrunus aviumt0
- Treatment
- ABA and ethephon treatmentse0
- Gene
- PacNCED1e0
- Outcome
- expressione0
- Direction
- enhancee0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to the control,ABA and ethephon treatments could enhance the expression of PacNCED1 in fruit”
t0“Sweet Cherry Fruit”
Claim · model-written summary
NDGA and IAA treatments had no effect on PacNCED1 expression in fruit.
- Taxon
- Sweet CherryPrunus aviumt0
- Treatment
- NDGA and IAA treatmentse0
- Gene
- PacNCED1e1
- Outcome
- expressione1
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while the NDGA and IAA treatments had no effect”
e1“expression of PacNCED1 in fruit”
t0“Sweet Cherry Fruit”
Claim · model-written summary
PacACO1 expression was undetected during sweet cherry fruit development but induced by ethephon and ABA.
- Taxon
- sweet cherryPrunus aviume0
- Treatment
- ethephon and ABA treatmentse0
- Gene
- PacACO1e0
- Outcome
- expressione0
- Direction
- not detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of PacACO1 during sweet cherry fruit development was not detected,but it could be induced with the ethephon and ABA treatments”
The same questions in other papers
(2-chloroethyl)phosphonic acid · molecular: expression · Prunus avium (sweet cherry)Results agree
1 paper in the same direction as this paper
- positive
(+)-abscisic acid · molecular: expression · Prunus avium (sweet cherry)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
drought · molecular: expression · Prunus avium (sweet cherry)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.