Abstract
Incorporation of the histone variant H2A.Z into nucleosomes by the SWR1 chromatin remodeling complex is a critical step in eukaryotic gene regulation. In Arabidopsis, SWR1c and H2A.Z have been shown to control gene expression underlying development and environmental responses. Although they have been implicated in defense, the specific roles of the complex subunits and H2A.Z in immunity are not well understood. In this study, we analyzed the roles of the SWR1c subunits, PHOTOPERIOD-INDEPENDENT EARLY FLOWERING1 (PIE1), ACTIN-RELATED PROTEIN6 (ARP6), and SWR1 COMPLEX 6 (SWC6), as well as H2A.Z, in defense and gene regulation. We found that SWR1c components play different roles in resistance to different pathogens. Loss of PIE1 and SWC6 function as well as depletion of H2A.Z led to reduced basal resistance, while loss of ARP6 fucntion resulted in enhanced resistance. We found that mutations in PIE1 and SWC6 resulted in impaired effector-triggered immunity. Mutation in SWR1c components and H2A.Z also resulted in compromised jasmonic acid/ethylene-mediated immunity. Genome-wide expression analyses similarly reveal distinct roles for H2A.Z and SWR1c components in gene regulation, and suggest a potential role for PIE1 in the regulation of the cross talk between defense signaling pathways. Our data show that although they are part of the same complex, Arabidopsis SWR1c components could have non-redundant functions in plant immunity and gene regulation.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Loss of PIE1 reduced basal resistance.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Loss of PIE1e1
- Gene
- PIE1e1
- Outcome
- basal resistancee1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis”
e1“Loss of PIE1 and SWC6 function as well as depletion of H2A.Z led to reduced basal resistance”
Claim · model-written summary
Loss of SWC6 reduced basal resistance.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Loss of PIE1 and SWC6 functione1
- Gene
- SWC6e1
- Outcome
- basal resistancee1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis”
e1“Loss of PIE1 and SWC6 function as well as depletion of H2A.Z led to reduced basal resistance”
Claim · model-written summary
H2A.Z depletion reduced basal resistance.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- depletion of H2A.Ze1
- Gene
- H2A.Ze1
- Outcome
- basal resistancee1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis”
e1“depletion of H2A.Z led to reduced basal resistance”
Claim · model-written summary
Loss of ARP6 enhanced resistance.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- loss of ARP6 fucntione1
- Gene
- ARP6e1
- Outcome
- resistancee1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis”
e1“loss of ARP6 fucntion resulted in enhanced resistance”
Claim · model-written summary
PIE1 mutation impaired effector-triggered immunity.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- mutations in PIE1e1
- Gene
- PIE1e1
- Outcome
- effector-triggered immunitye1
- Direction
- impairede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis”
e1“mutations in PIE1 and SWC6 resulted in impaired effector-triggered immunity”
Claim · model-written summary
SWC6 mutation impaired effector-triggered immunity.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- mutations in PIE1 and SWC6e1
- Gene
- SWC6e1
- Outcome
- effector-triggered immunitye1
- Direction
- impairede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis”
e1“mutations in PIE1 and SWC6 resulted in impaired effector-triggered immunity”
Claim · model-written summary
Mutations in SWR1c components and H2A.Z compromised jasmonic acid/ethylene-mediated immunity.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Mutation in SWR1c components and H2A.Ze1
- Gene
- SWR1c components and H2A.Ze1
- Outcome
- jasmonic acid/ethylene-mediated immunitye1
- Direction
- compromisede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis”
e1“Mutation in SWR1c components and H2A.Z also resulted in compromised jasmonic acid/ethylene-mediated immunity”
Claim · model-written summary
Genome-wide expression revealed distinct roles for H2A.Z and SWR1c components in gene regulation.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- H2A.Z and SWR1c componentse1
- Outcome
- distinct roles for H2A.Z and SWR1c components in gene regulatione1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis”
e1“Genome-wide expression analyses similarly reveal distinct roles for H2A.Z and SWR1c components in gene regulation”
Claim · model-written summary
PIE1 may regulate cross talk between defense signaling pathways.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- PIE1e1
- Outcome
- regulation of the cross talk between defense signaling pathwayse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis”
e1“suggest a potential role for PIE1 in the regulation of the cross talk between defense signaling pathways”
The same questions in other papers
light · disease · ArabidopsisPositive and negative results
10 papers in the same direction as this paper · 10 in the opposite direction
- positive
- positive
- positive
17 papers more in this group
See all 21 papers →loss of function · physiology: hormones · ArabidopsisPositive and negative results
51 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
56 papers more in this group
See all 60 papers →loss of function · disease · ArabidopsisPositive and negative results
100 papers in the same direction as this paper · 46 in the opposite direction
- negative
- negative
- positive
143 papers more in this group
See all 147 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.