Abstract
Axillary buds of `Valiant' grapevine ( Vitis spp.) grown in vitro were transferred onto Murashige and Skoog (MS) medium supplemented with different cytokinin and auxin combinations and concentrations. It was found that culture medium caused statistically important differences in number of nodes, number of fully expanded leaves, number of multiple shoots, number of roots, and length of shoots. MS medium supplemented with 1.0 mg BA/liter in combination with 0.01 mg NAA/L was found to be the best medium for shoot growth and callus production. MS medium supplemented with the combination of 0.5 mg BA/L and 0.01 mg NAA/L was the best medium for explant rooting. The medium containing BA and NAA encouraged better shoot growth than those containing BA alone. When the concentration of BA in the medium was increased, multiple shoot proliferation and teratological structures of explants increased, but the number of small leaves and length of internode decreased. Axillary bud culture led to better shoot growth than was found for shoot apex culture. The presence of leaves positively affected shoot growth from axillary buds. Also placing the axillary buds horizontally onto the medium gave better shoot proliferation and growth than placing them vertically.
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Claims 10
Claim · model-written summary
Culture medium affected nodes, expanded leaves and multiple shoots.
- Treatment
- culture mediume0
- Outcome
- number of nodes, number of fully expanded leaves, number of multiple shootse0
VitisNot reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“culture medium caused statistically important differences in number of nodes, number of fully expanded leaves, number of multiple shoots”
t0“Vitis”
Claim · model-written summary
Culture medium affected root number and shoot length.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“culture medium caused statistically important differences in number of nodes, number of fully expanded leaves, number of multiple shoots, number of roots, and length of shoots”
t0“Vitis”
Claim · model-written summary
Medium with 1.0 mg BA/liter and 0.01 mg NAA/L was best for shoot growth and callus production.
- Treatment
- 1.0 mg BA/liter in combination with 0.01 mg NAA/Le0
- Outcome
- shoot growth and callus productione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 1.0 mg BA/liter in combination with 0.01 mg NAA/L was found to be the best medium for shoot growth and callus production”
t0“Vitis”
Claim · model-written summary
Medium with 0.5 mg BA/L and 0.01 mg NAA/L was best for explant rooting.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with the combination of 0.5 mg BA/L and 0.01 mg NAA/L was the best medium for explant rooting”
t0“Vitis”
Claim · model-written summary
BA and NAA together improved shoot growth compared with BA alone.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The medium containing BA and NAA encouraged better shoot growth than those containing BA alone”
t0“Vitis”
Claim · model-written summary
Higher BA concentration increased multiple shoot proliferation and teratological structures.
- Treatment
- concentration of BA in the mediume0
- Outcome
- multiple shoot proliferation and teratological structures of explantse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When the concentration of BA in the medium was increased, multiple shoot proliferation and teratological structures of explants increased”
t0“Vitis”
Claim · model-written summary
Higher BA concentration decreased small leaf number and internode length.
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the number of small leaves and length of internode decreased”
t0“Vitis”
Claim · model-written summary
Axillary bud culture gave better shoot growth than shoot apex culture.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Axillary bud culture led to better shoot growth than was found for shoot apex culture”
t0“Vitis”
Claim · model-written summary
Leaves positively affected shoot growth from axillary buds.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of leaves positively affected shoot growth from axillary buds”
t0“Vitis”
Claim · model-written summary
Horizontal axillary bud placement improved shoot proliferation and growth over vertical placement.
- Treatment
- placing the axillary buds horizontally onto the mediume0
- Outcome
- shoot proliferation and growthe0
- Direction
- bettere0
- Comparator
- placing them verticallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“placing the axillary buds horizontally onto the medium gave better shoot proliferation and growth than placing them vertically”
t0“Vitis”
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