Abstract
The immature embryo of five maize lines (Zea mays.L) was used to study the influencing of genotypes, 2,4-D concentration and embryo-age on callus induction, influencing of 6-BA on callus differentiation ,and influencing of IBA on buds rooting. The results showed that, callis were obtained from Q31×Z31,Q31×Z3,B73,Z3 except Q31.In addition, there were dominant differences among the induction percentage of four genotypes on the same conditions.The concentration of 2,4-D in mediums and embryo-age had evident effects on the callus induction percentage of immature embryo. Adding 2.0mg/L 2,4-D in N6 medium and the embryo-age with 11d to 13d were optimal for improving the percentage of inducing callus. The 6-BA promoted callus differentiation and IBA promoted the buds rooting.
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Claims 8
Claim · model-written summary
Four maize genotypes produced callus, whereas Q31 did not.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callis were obtained from Q31×Z31,Q31×Z3,B73,Z3 except Q31”
e1“five maize lines (Zea mays.L)”
Claim · model-written summary
Callus induction percentages differed among the four genotypes under the same conditions.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there were dominant differences among the induction percentage of four genotypes on the same conditions”
e1“five maize lines (Zea mays.L)”
Claim · model-written summary
2,4-D concentration affected immature embryo callus induction percentage.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The concentration of 2,4-D in mediums and embryo-age had evident effects on the callus induction percentage of immature embryo”
e1“five maize lines (Zea mays.L)”
Claim · model-written summary
Embryo age affected immature embryo callus induction percentage.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“embryo-age had evident effects on the callus induction percentage of immature embryo”
e1“five maize lines (Zea mays.L)”
Claim · model-written summary
N6 medium with 2.0mg/L 2,4-D was optimal for raising callus induction percentage.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adding 2.0mg/L 2,4-D in N6 medium and the embryo-age with 11d to 13d were optimal for improving the percentage of inducing callus”
e1“five maize lines (Zea mays.L)”
Claim · model-written summary
Embryo age of 11d to 13d was optimal for raising callus induction percentage.
- Organ / stage
- embryo-agee0
- Outcome
- percentage of inducing calluse0
- Direction
- improvinge0
- Value
- 11d to 13de0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the embryo-age with 11d to 13d were optimal for improving the percentage of inducing callus”
e1“five maize lines (Zea mays.L)”
Claim · model-written summary
6-BA promoted callus differentiation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 6-BA promoted callus differentiation”
e1“five maize lines (Zea mays.L)”
Claim · model-written summary
IBA promoted bud rooting.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IBA promoted the buds rooting”
e1“five maize lines (Zea mays.L)”
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