Abstract
H2O2 burst in the defense responses of Wheat (Triticum aestivum L.) infected with compatible and incompatible leaf rust fungus races (Puccinia recondita f.sp. tritici) was investigated firstly. Cytofluorimetric analysis using the fluorescent probe DCFHDA scrutinized the generation of H2O2 in wheat primary leaves. The results indicated that H2O2 burst could be induced by either compatible or incompatible leaf rust fungus. However, the kinetics of H2O2 burst in incompatible interaction were biphasic, and the peak of phase II (20h after inoculation) was much higher than that of phase I (12h after inoculation), whereas, there only existed the weak and transient phase I H2O2 accumulation at 12h after inoculation in compatible interactions. In addition, pharmacological experiments were carried out. Antioxidants (AsA, DTT) and inhibitors of the mammalian neutrophil NADPH oxidase (DPI, imidazole) were separately injected into wheat primary leaves before inoculation to study their effects on hypersensitive reaction (HR) and H2O2 production in incompatible interaction. It was observed that treatments of wheat leaves with AsA, DTT, DPI or imidazole obviously suppressed the two peaks of intracellular H2O2 burst, whereas the second peak was inhibited much more. Moreover, the area of hypersensitively dead cells was decreased in the four treatments. These results suggested that NADPH oxidase may play an important role in H2O2 generation during wheat-leaf rust fungus interaction, and H2O2 burst may be involved in HR caused by leaf rust invading.
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Claims 10
Claim · model-written summary
Both compatible and incompatible leaf rust races induced H2O2 bursts in wheat.
- Taxon
- wheat
Triticum aestivumt0 - Interactor
- leaf rust funguse0pathogen
- Outcome
- H2O2 burste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H2O2 burst could be induced by either compatible or incompatible leaf rust fungus”
t0“wheat”
Claim · model-written summary
In incompatible interactions, the second H2O2 peak at 20 hours was much higher than the first peak at 12 hours.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- leaf rust fungust1pathogen
- Outcome
- peak of phase IIe0
- Direction
- much highere0
- Qualifier
- 20h after inoculatione0
- Comparator
- phase Ie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the peak of phase II (20h after inoculation) was much higher than that of phase I (12h after inoculation)”
t0“wheat”
t1“leaf rust fungus”
Claim · model-written summary
Compatible interactions showed only a weak transient first H2O2 accumulation at 12 hours.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- leaf rust fungust1pathogen
- Outcome
- weak and transient phase I H2O2 accumulatione0
- Qualifier
- 12h after inoculation in compatible interactionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there only existed the weak and transient phase I H2O2 accumulation at 12h after inoculation in compatible interactions”
t0“wheat”
t1“leaf rust fungus”
Claim · model-written summary
AsA suppressed both intracellular H2O2 peaks, especially the second.
- Taxon
- wheatTriticum aestivume0
- Treatment
- AsAe0
- Outcome
- two peaks of intracellular H2O2 burste0
- Direction
- suppressede0
- Qualifier
- second peak was inhibited much moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatments of wheat leaves with AsA, DTT, DPI or imidazole obviously suppressed the two peaks of intracellular H2O2 burst, whereas the second peak was inhibited much more”
Claim · model-written summary
DTT suppressed both intracellular H2O2 peaks, especially the second.
- Taxon
- wheatTriticum aestivume0
- Treatment
- DTTe0
- Outcome
- two peaks of intracellular H2O2 burste0
- Direction
- suppressede0
- Qualifier
- second peak was inhibited much moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatments of wheat leaves with AsA, DTT, DPI or imidazole obviously suppressed the two peaks of intracellular H2O2 burst, whereas the second peak was inhibited much more”
Claim · model-written summary
DPI suppressed both intracellular H2O2 peaks, especially the second.
- Taxon
- wheatTriticum aestivume0
- Treatment
- DPIe0
- Outcome
- two peaks of intracellular H2O2 burste0
- Direction
- suppressede0
- Qualifier
- second peak was inhibited much moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatments of wheat leaves with AsA, DTT, DPI or imidazole obviously suppressed the two peaks of intracellular H2O2 burst, whereas the second peak was inhibited much more”
Claim · model-written summary
Imidazole suppressed both intracellular H2O2 peaks, especially the second.
- Taxon
- wheatTriticum aestivume0
- Treatment
- imidazolee0
- Outcome
- two peaks of intracellular H2O2 burste0
- Direction
- suppressede0
- Qualifier
- second peak was inhibited much moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatments of wheat leaves with AsA, DTT, DPI or imidazole obviously suppressed the two peaks of intracellular H2O2 burst, whereas the second peak was inhibited much more”
Claim · model-written summary
Each of the four treatments reduced the area of hypersensitively dead cells.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- four treatmentse0
- Outcome
- area of hypersensitively dead cellse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the area of hypersensitively dead cells was decreased in the four treatments”
t0“wheat”
Claim · model-written summary
The authors suggest NADPH oxidase contributes to H2O2 generation in the interaction.
- Taxon
- wheatTriticum aestivume0
- Interactor
- leaf rust funguse0pathogen
- Gene
- NADPH oxidasee0
- Outcome
- H2O2 generatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NADPH oxidase may play an important role in H2O2 generation during wheat-leaf rust fungus interaction”
Claim · model-written summary
The authors suggest H2O2 bursts may contribute to the hypersensitive response during leaf rust invasion.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- leaf ruste0pathogen
- Outcome
- HRe0
- Qualifier
- H2O2 burste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H2O2 burst may be involved in HR caused by leaf rust invading”
t0“wheat”
The same questions in other papers
L-ascorbic acid · physiology: oxidative · Triticum aestivum (wheat)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
imidazole · physiology: oxidative · Triticum aestivum (wheat)Results agree
3 papers in the same direction as this paper
- negative
- negative
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.