Abstract
Salinity stress is a major abiotic stress to most plant including rice.It has been reported that gibberellic acid(GA3) can exert a natural beneficial effect on salt stressed rice.In this paper,the effect of salt stress on rice(Oryza sativa L.cv.Nipponbare) seed germination and the effect of GA3 on salt-stressed rice were investigated.A proteomic approach was employed to further understand the relieving effect of gibberellin on the inhibition of rice seed germination by salt stress.The 5-day-old rice seedlings were treated with H2O(control),5 g L-1 NaCl(treated group I),and 5 g L-1 NaCl + 100 μmol L-1 GA3(treated group II) for 48 h respectively.The proteins extracted from buds were separated by two-dimensional gel electrophoresis(2-DE) and analyzed with Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry(MALDI-TOF MS).The results showed that the seed germination of Nipponbare was inhibited by salt stress significantly,while GA3 could reduce the inhibition significantly.Four protein spots showed differential expression in 2-DE.Three of these proteins were down-regulated(spots 1-3) and one protein disappeared(spots 4) under salt stress.Expression levels of these proteins were recovered partly when treated with GA3 and NaCl at the same time.Two protein spots were identified as isoflavone reductase-like protein and phosphoglucomutase.These differential expression proteins may play important role in the mechanism of the relieving effect of gibberellin on the inhibition of rice germination by salt stress.
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Claims 8
Claim · model-written summary
Salt stress significantly inhibited Nipponbare seed germination.
- Taxon
- Rice
Oryza sativat0 - Material
- Nipponbaree0
- Treatment
- salt stresse0
- Outcome
- seed germinatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the seed germination of Nipponbare was inhibited by salt stress significantly”
t0“Rice”
Claim · model-written summary
GA3 significantly reduced salt inhibition of germination.
- Taxon
- RiceOryza sativat0
- Treatment
- GA3e0
- Outcome
- inhibitione0
- Direction
- reducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GA3 could reduce the inhibition significantly”
t0“Rice”
Claim · model-written summary
Four protein spots were differentially expressed.
- Taxon
- RiceOryza sativat0
- Outcome
- protein spots showed differential expressione0
- Value
- Four protein spotse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four protein spots showed differential expression in 2-DE”
t0“Rice”
Claim · model-written summary
Three protein spots were downregulated under salt stress.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three of these proteins were down-regulated(spots 1-3) and one protein disappeared(spots 4) under salt stress”
t0“Rice”
Claim · model-written summary
One protein spot disappeared under salt stress.
- Taxon
- RiceOryza sativat0
- Treatment
- salt stresse0
- Outcome
- protein disappearede0
- Value
- one proteine0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“one protein disappeared(spots 4) under salt stress”
t0“Rice”
Claim · model-written summary
GA3 and NaCl partly recovered expression of the proteins.
- Taxon
- RiceOryza sativat0
- Treatment
- GA3 and NaCle0
- Outcome
- Expression levels of these proteinse0
- Direction
- recoverede0
- Qualifier
- partlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression levels of these proteins were recovered partly when treated with GA3 and NaCl at the same time”
t0“Rice”
Claim · model-written summary
Two spots were identified as isoflavone reductase-like protein and phosphoglucomutase.
- Taxon
- RiceOryza sativat0
- Gene
- isoflavone reductase-like protein and phosphoglucomutasee0
- Outcome
- protein spots were identifiede0
- Value
- Two protein spotse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two protein spots were identified as isoflavone reductase-like protein and phosphoglucomutase”
t0“Rice”
Claim · model-written summary
The differential proteins may contribute to gibberellin relief of salt inhibition.
- Taxon
- riceOryza sativae0
- Gene
- differential expression proteinse0
- Outcome
- relieving effect of gibberellin on the inhibition of rice germination by salt stresse0
- Qualifier
- maye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These differential expression proteins may play important role in the mechanism of the relieving effect of gibberellin on the inhibition of rice germination by salt stress”
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