Abstract
Using cDNA-AFLP technique,a transcript-derived fragment(TDF) classified into bHLH type transcription factor family that responds to low-Pi stress was identified.Alignment analysis showed that this TDF(TaHLH1 EST) shares a high similarity to OsPTF1,a bHLH type transcription factor gene in rice that responds also to the low-Pi stress.Based on bioinformatic analysis,the cDNA sequence corresponding to TaHLH1 EST was obtained.TabHLH1 has 2 209 bp-long of full length cDNA and encodes a 480 aa-long of polypeptide.TabHLH1 contains the conserved Basic-Helix-Loop-Helix motif,with the amino acid residues of 13,15,6,and 22,respectively.Alignment analysis revealed that the conserved domains among TaHLH1 and its homologs of rice,barley,and maize are highly identical.The expression level of TaHLH1 was induced by several abiotic stresses,including low-Pi,low-nitrogen,drought,and high salinity,but was not responded to stresses of low-K,low-Ca,abiscisic acid(ABA),and low temperature.The results in this study suggest that TaHLH1 is one member of bHLH type transcription factor family in wheat and possibly plays an important role on mediation the transduction of signals initiated by stresses of low-P,low-N,drought,and high salinity.
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Claims 10
Claim · model-written summary
A cDNA-AFLP transcript-derived fragment of the bHLH transcription factor family responds to low-Pi stress in wheat.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- low-Pi stresse1
- Outcome
- transcript-derived fragmente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“bHLH type transcription factor TaHLH1in wheat”
e1“Using cDNA-AFLP technique,a transcript-derived fragment(TDF) classified into bHLH type transcription factor family that responds to low-Pi stress was identified”
Claim · model-written summary
The TaHLH1 EST shares high similarity with the rice bHLH gene OsPTF1.
- Taxon
- wheatTriticum aestivumt0
- Gene
- OsPTF1e2
- Outcome
- similaritye2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“bHLH type transcription factor TaHLH1in wheat”
e2“Alignment analysis showed that this TDF(TaHLH1 EST) shares a high similarity to OsPTF1,a bHLH type transcription factor gene in rice that responds also to the low-Pi stress”
Claim · model-written summary
TabHLH1 full-length cDNA is 2 209 bp long.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TabHLH1e3
- Outcome
- full length cDNAe3
- Value
- 2 209 bpe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“bHLH type transcription factor TaHLH1in wheat”
e3“TabHLH1 has 2 209 bp-long of full length cDNA and encodes a 480 aa-long of polypeptide”
Claim · model-written summary
TabHLH1 encodes a 480 aa polypeptide.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TabHLH1e3
- Outcome
- polypeptidee3
- Value
- 480 aae3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“bHLH type transcription factor TaHLH1in wheat”
e3“TabHLH1 has 2 209 bp-long of full length cDNA and encodes a 480 aa-long of polypeptide”
Claim · model-written summary
TabHLH1 contains the conserved Basic-Helix-Loop-Helix motif.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TabHLH1e4
- Outcome
- conserved Basic-Helix-Loop-Helix motife4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“bHLH type transcription factor TaHLH1in wheat”
e4“TabHLH1 contains the conserved Basic-Helix-Loop-Helix motif,with the amino acid residues of 13,15,6,and 22,respectively”
Claim · model-written summary
The conserved domains of TaHLH1 are highly identical to those of its rice, barley and maize homologs.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaHLH1e5
- Outcome
- conserved domainse5
- Qualifier
- highly identicale5
- Comparator
- homologs of rice,barley,and maizee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“bHLH type transcription factor TaHLH1in wheat”
e5“Alignment analysis revealed that the conserved domains among TaHLH1 and its homologs of rice,barley,and maize are highly identical”
Claim · model-written summary
TaHLH1 expression was induced by low-Pi, low-nitrogen, drought and high salinity in wheat.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- low-Pi,low-nitrogen,drought,and high salinitye6
- Gene
- TaHLH1e6
- Outcome
- expression level of TaHLH1e6
- Direction
- inducede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“bHLH type transcription factor TaHLH1in wheat”
e6“The expression level of TaHLH1 was induced by several abiotic stresses,including low-Pi,low-nitrogen,drought,and high salinity”
Claim · model-written summary
TaHLH1 expression was not responsive to low-K, low-Ca, ABA and low temperature in wheat.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- low-K,low-Ca,abiscisic acid(ABA),and low temperaturee7
- Gene
- TaHLH1e6
- Outcome
- expression level of TaHLH1e6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“bHLH type transcription factor TaHLH1in wheat”
e6“The expression level of TaHLH1 was induced by several abiotic stresses,including low-Pi,low-nitrogen,drought,and high salinity”
e7“but was not responded to stresses of low-K,low-Ca,abiscisic acid(ABA),and low temperature”
Claim · model-written summary
TaHLH1 possibly mediates signal transduction initiated by low-P, low-N, drought and high salinity stresses in wheat.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaHLH1e0
- Outcome
- mediation the transduction of signalse0
- Qualifier
- possiblye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“bHLH type transcription factor TaHLH1in wheat”
e0“The results in this study suggest that TaHLH1 is one member of bHLH type transcription factor family in wheat and possibly plays an important role on mediation the transduction of signals initiated by stresses of low-P,low-N,drought,and high salinity”
Claim · model-written summary
TaHLH1 is one member of the bHLH type transcription factor family in wheat.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaHLH1e0
- Outcome
- one member of bHLH type transcription factor familye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“bHLH type transcription factor TaHLH1in wheat”
e0“The results in this study suggest that TaHLH1 is one member of bHLH type transcription factor family in wheat and possibly plays an important role on mediation the transduction of signals initiated by stresses of low-P,low-N,drought,and high salinity”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.