Abstract
The open reading frame(ORF)of lysine decarboxylase(LDC)was isolated and identified from chilling tolerance cucumber'Chipper'using RT-PCR technique,named as CsLDC.The accession number was KC202438 in GenBank.The gene had an ORF of 645 bp encoding a protein of 215 amino acids,had a relative molecular mass of 23 625.2,and its isoelectric point was 5.99.The putative amino acid sequence only contained LDC conserved sequence,and shared high identity with LDC in Popilus trichocarpa.CsLDC had the nearest genetic relationship with Petunia × hybrida by phylogenetic tree analysis.The predicted protein had no signal peptide,but had an obvious transmembrane domain and multifunction site.The similartity of forecasting 3D structure of CsLDC with SWISS-MODEL and that of 2a33 encoded by AtLDC(At2G37210)was 88.89%.The responses of CsLDC expression to low temperature and salt stress was quick by quantitative real-time PCR,and the expression of CsLDC under treatments for 4 h reached the peak,which were 4.07 and 2.03 times of that controls,then dropped to the previous levels and maintained stable.But CsLDC was inhabited by drought,and got back the original when treated for 24 h.It was speculated that the CsLDC gene might be involved in the defence against low temperature and salt resistance in cucumber seedling.
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Claims 16
Claim · model-written summary
CsLDC was isolated and identified from Chipper cucumber.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The open reading frame(ORF)of lysine decarboxylase(LDC)was isolated and identified from chilling tolerance cucumber'Chipper'using RT-PCR technique,named as CsLDC”
e7“chilling tolerance cucumber'Chipper'”
Claim · model-written summary
CsLDC has a 645 bp ORF.
- Taxon
- cucumber
Cucumis sativuse7 - Gene
- CsLDCe1
- Outcome
- ORFe0
- Value
- 645 bpe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gene had an ORF of 645 bp”
t0“Cucumis sativus L.”
e7“chilling tolerance cucumber'Chipper'”
e1“named as CsLDC”
Claim · model-written summary
CsLDC encodes a protein of 215 amino acids.
- Taxon
- cucumberCucumis sativuse7
- Gene
- CsLDCe1
- Outcome
- proteine0
- Value
- 215 amino acidse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“encoding a protein of 215 amino acids”
t0“Cucumis sativus L.”
e7“chilling tolerance cucumber'Chipper'”
e1“named as CsLDC”
Claim · model-written summary
The predicted protein has relative molecular mass 23 625.2.
- Taxon
- cucumberCucumis sativuse7
- Gene
- CsLDCe1
- Outcome
- relative molecular masse0
- Value
- 23 625.2e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“had a relative molecular mass of 23 625.2”
t0“Cucumis sativus L.”
e7“chilling tolerance cucumber'Chipper'”
e1“named as CsLDC”
Claim · model-written summary
The predicted protein isoelectric point is 5.99.
- Taxon
- cucumberCucumis sativuse7
- Gene
- CsLDCe1
- Outcome
- isoelectric pointe0
- Value
- 5.99e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its isoelectric point was 5.99”
t0“Cucumis sativus L.”
e7“chilling tolerance cucumber'Chipper'”
e1“named as CsLDC”
Claim · model-written summary
The amino acid sequence contained an LDC conserved sequence.
- Taxon
- cucumberCucumis sativuse7
- Gene
- LDCe0
- Outcome
- LDC conserved sequencee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The putative amino acid sequence only contained LDC conserved sequence”
t0“Cucumis sativus L.”
e7“chilling tolerance cucumber'Chipper'”
Claim · model-written summary
The sequence had high identity with Popilus trichocarpa LDC.
- Taxon
- cucumberCucumis sativuse7
- Gene
- LDCe0
- Outcome
- high identitye0
- Comparator
- Popilus trichocarpae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shared high identity with LDC in Popilus trichocarpa”
t0“Cucumis sativus L.”
e7“chilling tolerance cucumber'Chipper'”
Claim · model-written summary
Phylogenetic analysis placed CsLDC closest to Petunia × hybrida.
- Taxon
- cucumberCucumis sativuse7
- Gene
- CsLDCe0
- Outcome
- nearest genetic relationshipe0
- Comparator
- Petunia × hybridae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsLDC had the nearest genetic relationship with Petunia × hybrida by phylogenetic tree analysis”
t0“Cucumis sativus L.”
e7“chilling tolerance cucumber'Chipper'”
Claim · model-written summary
The predicted protein had no signal peptide.
- Taxon
- cucumberCucumis sativuse7
- Gene
- CsLDCe1
- Outcome
- no signal peptidee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The predicted protein had no signal peptide”
t0“Cucumis sativus L.”
e7“chilling tolerance cucumber'Chipper'”
e1“named as CsLDC”
Claim · model-written summary
The predicted protein had a transmembrane domain and multifunction site.
- Taxon
- cucumberCucumis sativuse7
- Gene
- CsLDCe1
- Outcome
- transmembrane domain and multifunction sitee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“had an obvious transmembrane domain and multifunction site”
t0“Cucumis sativus L.”
e7“chilling tolerance cucumber'Chipper'”
e1“named as CsLDC”
Claim · model-written summary
The predicted CsLDC structure was 88.89% similar to the AtLDC encoded 2a33 structure.
- Taxon
- cucumberCucumis sativuse7
- Gene
- CsLDCe0
- Outcome
- similartity of forecasting 3D structuree0
- Value
- 88.89%e0
- Comparator
- 2a33 encoded by AtLDC(At2G37210)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The similartity of forecasting 3D structure of CsLDC with SWISS-MODEL and that of 2a33 encoded by AtLDC(At2G37210)was 88.89%”
t0“Cucumis sativus L.”
e7“chilling tolerance cucumber'Chipper'”
Claim · model-written summary
Low temperature raised CsLDC expression to a peak 4.07 times control at 4 hours.
- Taxon
- cucumberCucumis sativuse7
- Treatment
- low temperaturee1
- Gene
- CsLDCe0
- Outcome
- expression of CsLDCe0
- Direction
- peake0
- Value
- 4.07e0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of CsLDC under treatments for 4 h reached the peak,which were 4.07 and 2.03 times of that controls”
t0“Cucumis sativus L.”
e7“chilling tolerance cucumber'Chipper'”
e1“CsLDC expression to low temperature and salt stress was quick”
Claim · model-written summary
Salt stress raised CsLDC expression to a peak 2.03 times control at 4 hours.
- Taxon
- cucumberCucumis sativuse7
- Treatment
- salt stresse1
- Gene
- CsLDCe0
- Outcome
- expression of CsLDCe0
- Direction
- peake0
- Value
- 2.03e0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of CsLDC under treatments for 4 h reached the peak,which were 4.07 and 2.03 times of that controls”
t0“Cucumis sativus L.”
e7“chilling tolerance cucumber'Chipper'”
e1“CsLDC expression to low temperature and salt stress was quick”
Claim · model-written summary
After the peak, CsLDC expression dropped to earlier levels and remained stable.
- Taxon
- cucumberCucumis sativuse7
- Gene
- CsLDCe2
- Outcome
- CsLDC expressione1
- Direction
- droppede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“then dropped to the previous levels and maintained stable”
t0“Cucumis sativus L.”
e7“chilling tolerance cucumber'Chipper'”
e1“The responses of CsLDC expression to low temperature”
e2“named as CsLDC”
Claim · model-written summary
Drought inhibited CsLDC expression, which recovered after 24 hours.
- Taxon
- cucumberCucumis sativuse7
- Treatment
- droughte0
- Gene
- CsLDCe0
- Outcome
- CsLDCe0
- Direction
- inhabitede0
- Qualifier
- 24 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“But CsLDC was inhabited by drought,and got back the original when treated for 24 h”
t0“Cucumis sativus L.”
e7“chilling tolerance cucumber'Chipper'”
Claim · model-written summary
The authors speculate that CsLDC contributes to low temperature and salt resistance.
- Taxon
- cucumberCucumis sativuse7
- Gene
- CsLDCe0
- Outcome
- defence against low temperature and salt resistancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was speculated that the CsLDC gene might be involved in the defence against low temperature and salt resistance in cucumber seedling”
e7“chilling tolerance cucumber'Chipper'”
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.