Abstract
The plant height is an important trait in fruit tree. However, the molecular mechanism on dwarfism is still poorly understood. We found that colchicine-induced autotetraploid apple plants (Malus × domestica) exhibited a dwarf phenotype. The vertical length of cortical parenchyma cells was shorter in autotetraploids than in diploids, by observing paraffin sections. Hormone levels of indoleacetic acid (IAA) and brassinosteroid (BR) were significantly decreased in 3- and 5-year-old autotetraploid plants. Digital gene expression (DGE) analysis showed that the differentially expressed genes were mainly involved in IAA and BR pathways. microRNA390 was significantly upregulated according to microarray analysis. Exogenous application of IAA and BR promoted stem elongation of both apple plants grown in medium. The results show that dwarfing in autotetraploid apple plants is most likely regulated by IAA and BR. The dwarf phenotype of autotetraploid apple plants could be due to accumulation of miR390 after genome doubling, leading to upregulation of apple trans-acting short-interfering RNA 3 (MdTAS3) expression, which in turn downregulates the expression of MdARF3. Overall, this leads to partial interruption of the IAA and BR signal transduction pathway. Our study provides important insights into the molecular mechanisms underlying dwarfism in autopolyploid apple plants.
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Claims 6
Claim · model-written summary
colchicine-induced autotetraploid apple plants (Malus × domestica) exhibited a dwarf phenotype
- Taxon
- apple plants
Malus domesticae0 - Material
- autotetraploide0
- Outcome
- dwarf phenotypee0
Not reportedGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“colchicine-induced autotetraploid apple plants (Malus × domestica) exhibited a dwarf phenotype”
Claim · model-written summary
The vertical length of cortical parenchyma cells was shorter in autotetraploids than in diploids
- Taxon
- autotetraploidse0
- Outcome
- vertical length of cortical parenchyma cellse0
- Direction
- shortere0
- Comparator
- diploidse0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentSettingValueQualifier
Verbatim
e0“The vertical length of cortical parenchyma cells was shorter in autotetraploids than in diploids”
Claim · model-written summary
Hormone levels of indoleacetic acid (IAA) and brassinosteroid (BR) were significantly decreased in 3- and 5-year-old autotetraploid plants
- Taxon
- autotetraploid plantse0
- Outcome
- Hormone levels of indoleacetic acid (IAA) and brassinosteroid (BR)e0
- Direction
- decreasede0
- Qualifier
- 3- and 5-year-olde0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingValue
Verbatim
e0“Hormone levels of indoleacetic acid (IAA) and brassinosteroid (BR) were significantly decreased in 3- and 5-year-old autotetraploid plants”
Claim · model-written summary
Digital gene expression (DGE) analysis showed that the differentially expressed genes were mainly involved in IAA and BR pathways
Not reportedTaxonMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingDirectionValue
Verbatim
e0“Digital gene expression (DGE) analysis showed that the differentially expressed genes were mainly involved in IAA and BR pathways”
Claim · model-written summary
microRNA390 was significantly upregulated according to microarray analysis
Not reportedTaxonMaterialCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleTreatmentComparatorSettingValueQualifier
Verbatim
e0“microRNA390 was significantly upregulated according to microarray analysis”
Claim · model-written summary
Exogenous application of IAA and BR promoted stem elongation of both apple plants grown in medium
- Taxon
- apple plantsMalus domesticae0
- Treatment
- Exogenous application of IAA and BRe0
- Outcome
- stem elongatione0
- Direction
- promotede0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous application of IAA and BR promoted stem elongation of both apple plants grown in medium”
The same questions in other papers
indole-3-acetic acid · growth · Malus domestica (apple)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
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3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.