Paper · 2002 · PubMed

Cryopreservation of lipid-rich seeds: effect of moisture content and cooling rate on germination.

E M González-Benito; F Pérez-García

6
claims
7
verbatim quotes
9
citations
1
research areas
—
Not open access

Abstract

The effect of fast and slow cooling on germination of seeds from two Brassicaceae species (Eruca vesicaria (L.) Cav., Brassica napus L. var. oleifera (Moench) DC cv. Bingo) and cypselas from three Compositae species (Onopordum nervosum Boiss., Onopordum acanthium L., Helianthus annuus L. cv. Viky) at different moisture contents was studied. Seed lipid content (dry weight basis) ranged from 15% (O. nervosum) to 41% (H. annuus). For each species, seeds with four moisture contents were cryopreserved either by direct immersion in liquid nitrogen or by previous cooling at 10 degrees C/min from room temperature to -50 degrees C. In three species (E. vesicaria, B. napus, and H. annuus) germination of air-dried (6.2-8.9% moisture content) seeds cooled by direct immersion in liquid nitrogen was not significantly different from germination of control seeds (air-dried, non-cooled). In the two Onorpordum species the best response among cooling treatments was observed when air-dried seeds were slowly cooled.

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Claims 6

Observationobservedoriginal resultmeasured

Claim · model-written summary

Seed lipid content ranged from 15% in O. nervosum to 41% in H. annuus.

Taxon
O. nervosumOnopordum nervosume0
Outcome
Seed lipid contente0
Value
15%e0
Qualifier
41% (H. annuus)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Onopordum nervosum
Onopordum nervosum · TaxonRanko · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Seed lipid content (dry weight basis) ranged from 15% (O. nervosum) to 41% (H. annuus).”

✓ located verbatim in the source · abstract, characters 343–430 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Direct immersion in liquid nitrogen left germination of air-dried E. vesicaria seeds without a significant difference from non-cooled controls.

Taxon
E. vesicariaEruca vesicariae0
Treatment
direct immersion in liquid nitrogene0
Outcome
germinatione0
Direction
not significantly differente0
Qualifier
6.2-8.9% moisture contente0
Comparator
control seeds (air-dried, non-cooled)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eruca vesicaria
Eruca vesicaria · TaxonPatrick Hacker · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“In three species (E. vesicaria, B. napus, and H. annuus) germination of air-dried (6.2-8.9% moisture content) seeds cooled by direct immersion in liquid nitrogen was not significantly different from germination of control seeds (air-dried, non-cooled).”

✓ located verbatim in the source · abstract, characters 631–883 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Direct immersion in liquid nitrogen left germination of air-dried B. napus seeds without a significant difference from non-cooled controls.

Taxon
B. napusBrassica napuse0
Treatment
direct immersion in liquid nitrogene0
Outcome
germinatione0
Direction
not significantly differente0
Qualifier
6.2-8.9% moisture contente0
Comparator
control seeds (air-dried, non-cooled)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In three species (E. vesicaria, B. napus, and H. annuus) germination of air-dried (6.2-8.9% moisture content) seeds cooled by direct immersion in liquid nitrogen was not significantly different from germination of control seeds (air-dried, non-cooled).”

✓ located verbatim in the source · abstract, characters 631–883 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Direct immersion in liquid nitrogen left germination of air-dried H. annuus seeds without a significant difference from non-cooled controls.

Taxon
H. annuusHelianthus annuuse0
Treatment
direct immersion in liquid nitrogene0
Outcome
germinatione0
Direction
not significantly differente0
Qualifier
6.2-8.9% moisture contente0
Comparator
control seeds (air-dried, non-cooled)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“In three species (E. vesicaria, B. napus, and H. annuus) germination of air-dried (6.2-8.9% moisture content) seeds cooled by direct immersion in liquid nitrogen was not significantly different from germination of control seeds (air-dried, non-cooled).”

✓ located verbatim in the source · abstract, characters 631–883 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Air-dried O. nervosum seeds responded best among cooling treatments when slowly cooled.

Taxon
Onorpordum speciese0
Treatment
slowly coolede0
Outcome
best responsee0
Direction
beste0
Comparator
cooling treatmentse0

Verbatim

e0

“In the two Onorpordum species the best response among cooling treatments was observed when air-dried seeds were slowly cooled.”

✓ located verbatim in the source · abstract, characters 884–1010 · show in text ↑
t0

“Onopordum nervosum Boiss.”

✓ located verbatim in the source · abstract, characters 217–242 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Air-dried O. acanthium seeds responded best among cooling treatments when slowly cooled.

Taxon
Onorpordum speciese0
Treatment
slowly coolede0
Outcome
best responsee0
Direction
beste0
Comparator
cooling treatmentse0

Verbatim

e0

“In the two Onorpordum species the best response among cooling treatments was observed when air-dried seeds were slowly cooled.”

✓ located verbatim in the source · abstract, characters 884–1010 · show in text ↑

nitrogen · growth · Helianthus annuus (common sunflower)Results differ

76 papers: 63 positive, 13 negative or no effect

12 papers in the same direction as this paper · 63 in the opposite direction

  1. positive
  2. positive
  3. positive
    Nitrogen limitation reduced leaf area index in sunflower.

    Effects of nitrogen supply on canopy development of maize and sunflower · 2012

    Opposite directionAbout lacking this nutrient, so it counts the other way round

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application · growth · Eruca vesicariaPositive and negative results

3 papers: 1 positive, 2 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. no effect
See all 3 papers →

nitrogen · growth · Eruca vesicariaPositive and negative results

6 papers: 4 positive, 2 negative or no effect

1 paper in the same direction as this paper · 4 in the opposite direction

  1. positive
  2. positive
  3. positive

2 papers more in this group

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application · growth · Brassica napus (oilseed rape)Positive and negative results

47 papers: 38 positive, 9 negative or no effect

8 papers in the same direction as this paper · 38 in the opposite direction

  1. positive
  2. positive
  3. positive

43 papers more in this group

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application · growth · Helianthus annuus (common sunflower)Positive and negative results

81 papers: 73 positive, 8 negative or no effect

7 papers in the same direction as this paper · 73 in the opposite direction

  1. positive
  2. positive
  3. positive

77 papers more in this group

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nitrogen · growth · Brassica napus (oilseed rape)Positive and negative results

82 papers: 65 positive, 17 negative or no effect

16 papers in the same direction as this paper · 65 in the opposite direction

  1. positive
  2. positive
  3. positive

78 papers more in this group

See all 82 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.