Abstract
Table grapes (Vitis vinifera cv. Cardinal) are highly perishable and their quality deteriorates during postharvest storage at low temperature mainly because of sensitivity to fungal decay and senescence of rachis. The application of a 3-day CO2 treatment (20 kPa CO2 + 20 kPa O2 + 60 kPa N2) at 0°C reduced total decay and retained fruit quality in early and late-harvested table grapes during postharvest storage. In order to study the transcriptional responsiveness of table grapes to low temperature and high CO2 levels in the first stage of storage and how the maturity stage affect these changes, we have performed a comparative large-scale transcriptional analysis using the custom-made GrapeGen GeneChip®. In the first stage of storage, low temperature led to a significantly intense change in grape skin transcriptome irrespective of fruit maturity, although there were different changes within each stage. In the case of CO2 treated samples, in comparison to fruit at time zero, only slight differences were observed. Functional enrichment analysis revealed that major modifications in the transcriptome profile of early- and late-harvested grapes stored at 0°C are linked to biotic and abiotic stress-responsive terms. However, in both cases there is a specific reprogramming of the transcriptome during the first stage of storage at 0°C in order to withstand the cold stress. Thus, genes involved in gluconeogenesis, photosynthesis, mRNA translation and lipid transport were up-regulated in the case of early-harvested grapes, and genes related to protein folding stability and intracellular membrane trafficking in late-harvested grapes. The beneficial effect of high CO2 treatment maintaining table grape quality seems to be an active process requiring the induction of several transcription factors and kinases in early-harvested grapes, and the activation of processes associated to the maintenance of energy in late-harvested grapes.
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Claims 9
Claim · model-written summary
A three-day CO2 treatment reduced total decay in early- and late-harvested grapes during storage.
- Taxon
- table grapese0
- Treatment
- 3-day CO2 treatment (20 kPa CO2 + 20 kPa O2 + 60 kPa N2)e0
- Outcome
- total decaye0
- Direction
- reducede0
- Qualifier
- early and late-harvestede0
- Setting
- postharvest storagee0
Not reportedComparator
Verbatim
e0“The application of a 3-day CO2 treatment (20 kPa CO2 + 20 kPa O2 + 60 kPa N2) at 0°C reduced total decay and retained fruit quality in early and late-harvested table grapes during postharvest storage.”
Claim · model-written summary
The same CO2 treatment retained fruit quality in both harvest stages.
- Taxon
- table grapese0
- Treatment
- 3-day CO2 treatment (20 kPa CO2 + 20 kPa O2 + 60 kPa N2)e0
- Outcome
- fruit qualitye0
- Direction
- retainede0
- Qualifier
- early and late-harvestede0
Not reportedComparator
Verbatim
e0“The application of a 3-day CO2 treatment (20 kPa CO2 + 20 kPa O2 + 60 kPa N2) at 0°C reduced total decay and retained fruit quality in early and late-harvested table grapes during postharvest storage.”
Claim · model-written summary
Low temperature significantly altered grape skin transcriptomes at both maturity stages, with stage-specific changes.
- Taxon
- Table grapese1
- Organ / stage
- grape skine0
- Treatment
- low temperaturee0
- Outcome
- grape skin transcriptomee0
- Qualifier
- irrespective of fruit maturitye0
Not reportedComparatorDirection
Verbatim
e0“low temperature led to a significantly intense change in grape skin transcriptome irrespective of fruit maturity, although there were different changes within each stage.”
e1“Table grapes (Vitis vinifera cv. Cardinal)”
Claim · model-written summary
CO2-treated fruit had only slight transcriptome differences from fruit at time zero.
Not reportedDirection
Verbatim
e0“In the case of CO2 treated samples, in comparison to fruit at time zero, only slight differences were observed.”
e1“Table grapes (Vitis vinifera cv. Cardinal)”
Claim · model-written summary
Transcriptome changes during cold storage were linked to biotic and abiotic stress responses.
- Taxon
- grapese0
- Treatment
- stored at 0°Ce0
- Outcome
- modifications in the transcriptome profilee0
- Qualifier
- biotic and abiotic stress-responsive termse0
Not reportedDirection
Verbatim
e0“major modifications in the transcriptome profile of early- and late-harvested grapes stored at 0°C are linked to biotic and abiotic stress-responsive terms.”
Claim · model-written summary
Early-harvested grapes upregulated genes involved in gluconeogenesis, photosynthesis, mRNA translation and lipid transport during cold storage.
Verbatim
e0“genes involved in gluconeogenesis, photosynthesis, mRNA translation and lipid transport were up-regulated in the case of early-harvested grapes”
Claim · model-written summary
Late-harvested grapes upregulated genes related to protein folding and membrane trafficking during cold storage.
Verbatim
e0“genes related to protein folding stability and intracellular membrane trafficking in late-harvested grapes.”
e1“genes involved in gluconeogenesis, photosynthesis, mRNA translation and lipid transport were up-regulated in the case of early-harvested grapes”
Claim · model-written summary
The authors interpret CO2-based quality maintenance in early-harvested grapes as requiring transcription factor and kinase induction.
Verbatim
e0“The beneficial effect of high CO2 treatment maintaining table grape quality seems to be an active process requiring the induction of several transcription factors and kinases in early-harvested grapes”
Claim · model-written summary
The authors interpret CO2-based quality maintenance in late-harvested grapes as involving energy-maintenance processes.
Verbatim
e0“the activation of processes associated to the maintenance of energy in late-harvested grapes.”
e1“The beneficial effect of high CO2 treatment maintaining table grape quality seems to be an active process requiring the induction of several transcription factors and kinases in early-harvested grapes”
The same questions in other papers
co2 · quality: grain · VitisPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
co2 · disease · VitisPositive and negative results
8 papers in the same direction as this paper · 1 in the opposite direction · 2 reporting no difference from an alternative
- positive
- positive
- no effect
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.