Abstract
Strawberry fruits are perishable due to fruit softening occurring during storage and marketing, but the mechanism by which this occurs is not clear. In this study, the possible mechanism of softening is explored by comparative studies on the changes of fruit firmness, pectate lyase (PL) activities, and relative expression levels of PL genes between two strawberry cultivars. The results indicate that a method for the determination of PL activity in the strawberry fruit was established. The activity of PL was at a low level in the small green fruit to white fruit stage, and maintained at a high level in the ripening and softening stage. The PL gene was specifically expressed in fruit and mainly in the ripening stage. PL expressions were closely correlated with PL activities, and negatively related with the changes in flesh firmness. The full-length cDNA of PL genes from the two cultivars was cloned using the RACE method. Amino acid residues of C terminal sequences of the PL proteins from different species showed significant variations. Those results suggested that the differences in PL activity, gene sequence, and gene expression patterns may lead to the different roles of PL in different fruit textures of strawberries during ripening and softening.
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Claims 9
Claim · model-written summary
A method for measuring pectate lyase activity in strawberry fruit was established.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae1 - Outcome
- determination of PL activitye1
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e1“a method for the determination of PL activity in the strawberry fruit was established”
Claim · model-written summary
PL activity was low from small green to white fruit stages.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- small green fruit to white fruit stagee1
- Gene
- PLe1
- Outcome
- activity of PLe1
- Direction
- low levele1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“strawberry fruits”
e1“The activity of PL was at a low level in the small green fruit to white fruit stage”
Claim · model-written summary
PL activity stayed high through ripening and softening.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- ripening and softening stagee1
- Outcome
- high levele1
- Direction
- high levele1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“strawberry fruits”
e1“maintained at a high level in the ripening and softening stage”
Claim · model-written summary
PL gene expression was fruit-specific and mainly occurred during ripening.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- fruite1
- Gene
- PL genee1
- Outcome
- specifically expressed in fruit and mainly in the ripening stagee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“strawberry fruits”
e1“The PL gene was specifically expressed in fruit and mainly in the ripening stage”
Claim · model-written summary
PL expression correlated closely with PL activity.
- Taxon
- strawberryFragaria ×ananassat0
- Gene
- PLe1
- Outcome
- PL activitiese1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“strawberry fruits”
e1“PL expressions were closely correlated with PL activities”
Claim · model-written summary
PL expression was negatively related to flesh firmness changes.
- Taxon
- strawberryFragaria ×ananassat0
- Gene
- PLe1
- Outcome
- changes in flesh firmnesse1
- Direction
- negatively relatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“strawberry fruits”
e1“PL expressions were closely correlated with PL activities, and negatively related with the changes in flesh firmness”
Claim · model-written summary
Full length PL cDNA was cloned from two strawberry cultivars.
- Taxon
- strawberryFragaria ×ananassat0
- Gene
- PL genese1
- Outcome
- full-length cDNAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“strawberry fruits”
e1“The full-length cDNA of PL genes from the two cultivars was cloned using the RACE method”
Claim · model-written summary
C terminal PL protein sequences varied significantly among species.
- Taxon
- strawberryFragaria ×ananassat0
- Gene
- PL proteinse1
- Outcome
- Amino acid residues of C terminal sequencese1
- Direction
- variationse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“strawberry fruits”
e1“Amino acid residues of C terminal sequences of the PL proteins from different species showed significant variations”
Claim · model-written summary
Authors proposed that PL activity, sequence and expression differences may explain texture variation during strawberry ripening.
- Taxon
- strawberryFragaria ×ananassat0
- Gene
- PLe1
- Outcome
- different fruit texturese1
- Qualifier
- differences in PL activity, gene sequence, and gene expression patternse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“strawberry fruits”
e1“the differences in PL activity, gene sequence, and gene expression patterns may lead to the different roles of PL in different fruit textures of strawberries during ripening and softening”