Abstract
In living organisms, daily light/dark cycles profoundly affect cellular processes. In plants, optimal growth and development, and adaptation to daily light-dark cycles, require starch synthesis and turnover. However, the underlying molecular mechanisms coordinating daily starch metabolism remain poorly understood. To explore the roles of Arabidopsis thaliana light signal transduction proteins FAR-RED ELONGATED HYPOCOTYLS3 (FHY3) and FAR-RED-IMPAIRED RESPONSE1 (FAR1) in starch metabolism, the contents of starch and water-soluble polysaccharides, and the structure of starch granules were investigated in fhy3, far1 and fhy3 far1 mutant plants. Disruption of FHY3 or FAR1 reduced starch accumulation and altered starch granule structure in the fhy3-4, far1-2, and fhy3-4 far1-2 mutant plants. Furthermore, molecular and genetic evidence revealed that the gene encoding the starch-debranching enzyme ISOAMYLASE2 (ISA2) is a direct target of FHY3 and FAR1, and functions in light-induced starch synthesis. Our data establish the first molecular link between light signal transduction and starch synthesis, suggesting that the light-signaling proteins FHY3 and FAR1 influence starch synthesis and starch granule formation through transcriptional activation of ISA2.
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Claims 9
Claim · model-written summary
FHY3 disruption reduced starch accumulation.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Material
- fhy3-4e0
- Treatment
- Disruption of FHY3e0
- Outcome
- starch accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of FHY3 or FAR1 reduced starch accumulation and altered starch granule structure in the fhy3-4, far1-2, and fhy3-4 far1-2 mutant plants.”
t0“Arabidopsis thaliana”
Claim · model-written summary
FAR1 disruption reduced starch accumulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- far1-2e0
- Treatment
- FAR1e0
- Outcome
- starch accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of FHY3 or FAR1 reduced starch accumulation and altered starch granule structure in the fhy3-4, far1-2, and fhy3-4 far1-2 mutant plants.”
t0“Arabidopsis thaliana”
Claim · model-written summary
The double mutant had reduced starch accumulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- fhy3-4 far1-2e0
- Treatment
- fhy3-4 far1-2e0
- Outcome
- starch accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of FHY3 or FAR1 reduced starch accumulation and altered starch granule structure in the fhy3-4, far1-2, and fhy3-4 far1-2 mutant plants.”
t0“Arabidopsis thaliana”
Claim · model-written summary
FHY3 disruption altered starch granule structure.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- fhy3-4e0
- Treatment
- Disruption of FHY3e0
- Outcome
- altered starch granule structuree0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of FHY3 or FAR1 reduced starch accumulation and altered starch granule structure in the fhy3-4, far1-2, and fhy3-4 far1-2 mutant plants.”
t0“Arabidopsis thaliana”
Claim · model-written summary
FAR1 disruption altered starch granule structure.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- far1-2e0
- Treatment
- FAR1e0
- Outcome
- altered starch granule structuree0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of FHY3 or FAR1 reduced starch accumulation and altered starch granule structure in the fhy3-4, far1-2, and fhy3-4 far1-2 mutant plants.”
t0“Arabidopsis thaliana”
Claim · model-written summary
The double mutant had altered starch granule structure.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- fhy3-4 far1-2e0
- Treatment
- fhy3-4 far1-2e0
- Outcome
- altered starch granule structuree0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of FHY3 or FAR1 reduced starch accumulation and altered starch granule structure in the fhy3-4, far1-2, and fhy3-4 far1-2 mutant plants.”
t0“Arabidopsis thaliana”
Claim · model-written summary
ISA2 is a direct target of FHY3 and FAR1.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- ISOAMYLASE2 (ISA2)e0
- Outcome
- a direct target of FHY3 and FAR1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the gene encoding the starch-debranching enzyme ISOAMYLASE2 (ISA2) is a direct target of FHY3 and FAR1, and functions in light-induced starch synthesis.”
t0“Arabidopsis thaliana”
Claim · model-written summary
ISA2 functions in light-induced starch synthesis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- ISOAMYLASE2 (ISA2)e0
- Outcome
- light-induced starch synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the gene encoding the starch-debranching enzyme ISOAMYLASE2 (ISA2) is a direct target of FHY3 and FAR1, and functions in light-induced starch synthesis.”
t0“Arabidopsis thaliana”
Claim · model-written summary
FHY3 and FAR1 influence starch synthesis and granule formation through ISA2 transcriptional activation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- FHY3 and FAR1e0
- Outcome
- starch synthesis and starch granule formatione0
- Qualifier
- transcriptional activation of ISA2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that the light-signaling proteins FHY3 and FAR1 influence starch synthesis and starch granule formation through transcriptional activation of ISA2.”
t0“Arabidopsis thaliana”
The same questions in other papers
loss of function · quality: sugar starch · Arabidopsis thaliana (thale cress)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.