Abstract
Small RNAs, including ta-siRNAs, play crucial roles in various processes in plants. Efforts have been made for decades to elucidate the biogenesis and function of ta-siRNAs. Though the key proteins involved in ta-siRNA biogenesis have been identified, the subcellular localization where ta-siRNAs are processed remains largely unexplored. Remarkably, non-coding TAS transcripts were reported to be bound by ribosomes, the machinery responsible for protein translation. Utilizing edited TAS genes in Arabidopsis , a combination of sRNA-seq, mRNA-seq, RIP-seq, and degradome-seq was employed to investigate the role of ribosomes in ta-siRNA biogenesis in this study. In the two-hit model, deletion of ribosome-binding regions resulted in a decrease in the abundance of intact TAS3 transcripts but did not significantly affect ta-siRNAs production or the efficiency of miRNA-guided cleavage. Conversely, the deletion of ribosome-binding regions led to a significant reduction in ta-siRNA abundance without affecting mRNA levels in the one-hit model. These findings indicate that in the two-hit model, ribosomes primarily stabilize TAS transcripts, while in the one-hit model, they suppress miRNA cleavage but facilitate subsequent processing. Collectively, this study proposes a model that ribosomes play distinct roles in the one-hit and two-hit models of ta-siRNA biogenesis, and provides a new angle to investigate the tangled connection between small RNAs, including miRNA and ta-siRNA, and translation.
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Claims 4
Claim · model-written summary
Deleting ribosome-binding regions decreased intact TAS3 transcripts in the two-hit model.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- deletion of ribosome-binding regionse0
- Gene
- TAS3e0
- Outcome
- abundance of intact TAS3 transcriptse0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the two-hit model, deletion of ribosome-binding regions resulted in a decrease in the abundance of intact TAS3 transcripts”
t0“Arabidopsis thaliana”
Claim · model-written summary
Deleting ribosome-binding regions did not significantly affect ta-siRNA production or miRNA-guided cleavage efficiency in the two-hit model.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- deletion of ribosome-binding regionse1
- Outcome
- ta-siRNAs production or the efficiency of miRNA-guided cleavagee0
- Direction
- did not significantly affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but did not significantly affect ta-siRNAs production or the efficiency of miRNA-guided cleavage.”
t0“Arabidopsis thaliana”
t1“Ribosomes binding to TAS transcripts buffer ta-siRNA biogenesis in Arabidopsis thaliana”
e1“In the two-hit model, deletion of ribosome-binding regions resulted in a decrease in the abundance of intact TAS3 transcripts”
Claim · model-written summary
Deleting ribosome-binding regions significantly reduced ta-siRNA abundance in the one-hit model.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- deletion of ribosome-binding regionse0
- Outcome
- ta-siRNA abundancee0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the deletion of ribosome-binding regions led to a significant reduction in ta-siRNA abundance without affecting mRNA levels in the one-hit model.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Deleting ribosome-binding regions did not affect mRNA levels in the one-hit model.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- deletion of ribosome-binding regionse0
- Outcome
- mRNA levelse0
- Direction
- without affectinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the deletion of ribosome-binding regions led to a significant reduction in ta-siRNA abundance without affecting mRNA levels in the one-hit model.”
t0“Arabidopsis thaliana”
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