Abstract
Carbon and nitrogen metabolism pathways are regulated by complex mechanisms in order to optimize growth and development of plants. This study was conducted to contribute to the determination of roles of some key enzymes of carbon and nitrogen metabolism in sorghum roots. Sorghum were grown under nitrate (NO3-), ammonium (NH 4+) or nitrate combined to ammonium at different concentrations. Growth parameters and protein content were evaluated. In addition, we analyzed key enzymes activities involved in nitrogen and carbon metabolism such as: nitrate reductase (NR), glutamine synthetase (GS), aspartate aminotransferase (AAT) and phosphoenolpyruvate carboxylase (PEPC). At high nitrogen levels (ammonium or nitrate), the sorghum roots showed an increase in biomass accompanied by an increase in protein content. Likewise, high concentrations induced accumulation of the non-photosynthetic isoform of phosphoenolpyruvate carboxylase. Glutamine synthetase and aspartate aminotransferase activities were also increased, especially at high levels of ammonium. These results showed that sorghum plants have an efficient system for nitrogen assimilation in their roots, allowing this plant to tolerate excessive concentration of this nutrient.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
High ammonium or nitrate increased sorghum root biomass.
- Organ / stage
- rootse0
- Treatment
- high nitrogen levels (ammonium or nitrate)e0
- Outcome
- biomasse0
- Direction
- increasee0
SorghumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At high nitrogen levels (ammonium or nitrate), the sorghum roots showed an increase in biomass accompanied by an increase in protein content”
Claim · model-written summary
High ammonium or nitrate increased sorghum root protein content.
- Organ / stage
- rootse0
- Treatment
- high nitrogen levels (ammonium or nitrate)e0
- Outcome
- protein contente0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At high nitrogen levels (ammonium or nitrate), the sorghum roots showed an increase in biomass accompanied by an increase in protein content”
Claim · model-written summary
High nitrogen concentrations induced accumulation of non-photosynthetic phosphoenolpyruvate carboxylase in sorghum.
- Treatment
- high concentrationse0
- Gene
- non-photosynthetic isoform of phosphoenolpyruvate carboxylasee0
- Outcome
- accumulation of the non-photosynthetic isoform of phosphoenolpyruvate carboxylasee0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high concentrations induced accumulation of the non-photosynthetic isoform of phosphoenolpyruvate carboxylase”
t0“Sorghum”
Claim · model-written summary
Glutamine synthetase activity increased, especially under high ammonium, in sorghum.
- Treatment
- high levels of ammoniume0
- Gene
- Glutamine synthetasee0
- Outcome
- Glutamine synthetasee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glutamine synthetase and aspartate aminotransferase activities were also increased, especially at high levels of ammonium”
t0“Sorghum”
Claim · model-written summary
Aspartate aminotransferase activity increased, especially under high ammonium, in sorghum.
- Treatment
- high levels of ammoniume0
- Gene
- aspartate aminotransferasee0
- Outcome
- aspartate aminotransferase activitiese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glutamine synthetase and aspartate aminotransferase activities were also increased, especially at high levels of ammonium”
t0“Sorghum”
Claim · model-written summary
The authors concluded that sorghum roots efficiently assimilate nitrogen and tolerate excess nitrogen.
- Outcome
- tolerate excessive concentration of this nutriente0
- Qualifier
- efficient system for nitrogen assimilation in their rootse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sorghum plants have an efficient system for nitrogen assimilation in their roots, allowing this plant to tolerate excessive concentration of this nutrient”
The same questions in other papers
nitrogen · quality: protein · SorghumResults differ
42 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- no effect
- negative
43 papers more in this group
See all 47 papers →nitrogen · growth · SorghumResults differ
80 papers in the same direction as this paper · 10 in the opposite direction · 2 reporting no difference from an alternative
- negative
- no effect
- no effect
89 papers more in this group
See all 93 papers →nitrogen · physiology: enzyme activity · SorghumPositive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
2 papers more in this group
See all 6 papers →nitrogen · molecular: metabolites · SorghumPositive and negative results
16 papers in the same direction as this paper · 6 in the opposite direction
- no effect
- negative
- negative
19 papers more in this group
See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.