Abstract
Summary Salt tolerance quantitative trait loci analysis of rice has revealed that the SKC 1 locus, which is involved in a higher K + /Na + ratio in shoots, corresponds to the Os HKT 1;5 gene encoding a Na + ‐selective transporter. However, physiological roles of Os HKT 1;5 in rice exposed to salt stress remain elusive, and no Os HKT 1;5 gene disruption mutants have been characterized to date. In this study, we dissected two independent T‐ DNA insertional Os HKT 1;5 mutants. Measurements of ion contents in tissues and 22 Na + tracer imaging experiments showed that loss‐of‐function of Os HKT 1;5 in salt‐stressed rice roots triggers massive Na + accumulation in shoots. Salt stress‐induced increases in the Os HKT 1;5 transcript were observed in roots and basal stems, including basal nodes. Immuno‐staining using an anti‐Os HKT 1;5 peptide antibody indicated that Os HKT 1;5 is localized in cells adjacent to the xylem in roots. Additionally, direct introduction of 22 Na + tracer to leaf sheaths also demonstrated the involvement of Os HKT 1;5 in xylem Na + unloading in leaf sheaths. Furthermore, Os HKT 1;5 was indicated to be present in the plasma membrane and found to localize also in the phloem of diffuse vascular bundles in basal nodes. Together with the characteristic 22 Na + allocation in the blade of the developing immature leaf in the mutants, these results suggest a novel function of Os HKT 1;5 in mediating Na + exclusion in the phloem to prevent Na + transfer to young leaf blades. Our findings further demonstrate that the function of Os HKT 1;5 is crucial over growth stages of rice, including the protection of the next generation seeds as well as of vital leaf blades under salt stress.
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Claims 7
Claim · model-written summary
Loss of function of OsHKT1;5 in salt-stressed rice roots triggers massive Na+ accumulation in shoots.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- rootse0
- Treatment
- loss‐of‐function of Os HKT 1;5e0
- Gene
- Os HKT 1;5e0
- Outcome
- Na + accumulation in shootse0
- Direction
- increaseinferrede0
- Qualifier
- salt‐stressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“loss‐of‐function of Os HKT 1;5 in salt‐stressed rice roots triggers massive Na + accumulation in shoots”
Claim · model-written summary
Salt stress increased the OsHKT1;5 transcript in roots and basal stems, including basal nodes.
- Taxon
- riceOryza sativat0
- Organ / stage
- roots and basal stemse0
- Treatment
- Salt stresse0
- Gene
- Os HKT 1;5e0
- Outcome
- Os HKT 1;5 transcripte0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Salt stress‐induced increases in the Os HKT 1;5 transcript were observed in roots and basal stems, including basal nodes”
Claim · model-written summary
OsHKT1;5 protein is localized in cells adjacent to the xylem in roots.
- Taxon
- riceOryza sativat0
- Organ / stage
- rootse0
- Gene
- Os HKT 1;5e0
- Outcome
- localized in cells adjacent to the xyleme0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Os HKT 1;5 is localized in cells adjacent to the xylem in roots”
Claim · model-written summary
Direct introduction of 22Na+ tracer to leaf sheaths demonstrated the involvement of OsHKT1;5 in xylem Na+ unloading in leaf sheaths.
- Taxon
- riceOryza sativat0
- Organ / stage
- leaf sheathse0
- Gene
- Os HKT 1;5e0
- Outcome
- xylem Na + unloadinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“direct introduction of 22 Na + tracer to leaf sheaths also demonstrated the involvement of Os HKT 1;5 in xylem Na + unloading in leaf sheaths”
Claim · model-written summary
OsHKT1;5 was indicated to be present in the plasma membrane and also in the phloem of diffuse vascular bundles in basal nodes.
- Taxon
- riceOryza sativat0
- Organ / stage
- phloem of diffuse vascular bundles in basal nodese0
- Gene
- Os HKT 1;5e0
- Outcome
- present in the plasma membranee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Os HKT 1;5 was indicated to be present in the plasma membrane and found to localize also in the phloem of diffuse vascular bundles in basal nodes”
Claim · model-written summary
The results suggest OsHKT1;5 mediates Na+ exclusion in the phloem, preventing Na+ transfer to young leaf blades.
- Taxon
- riceOryza sativat0
- Organ / stage
- young leaf bladese0
- Gene
- Os HKT 1;5e0
- Outcome
- Na + exclusion in the phloeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“suggest a novel function of Os HKT 1;5 in mediating Na + exclusion in the phloem to prevent Na + transfer to young leaf blades”
Claim · model-written summary
The function of OsHKT1;5 is crucial over the growth stages of rice, including protection of next-generation seeds and vital leaf blades under salt stress.
- Taxon
- riceOryza sativae0
- Treatment
- salt stresse0
- Gene
- Os HKT 1;5e0
- Outcome
- protection of the next generation seeds as well as of vital leaf bladese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“the function of Os HKT 1;5 is crucial over growth stages of rice, including the protection of the next generation seeds as well as of vital leaf blades under salt stress”
The same questions in other papers
loss of function · molecular: metabolites · Oryza sativa (rice)Positive and negative results
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