Abstract
Bacterial sigma factor E (SigE) is a positive regulator of sugar catabolism via its interaction with the H subunit of Mg-chelatase (CHLH), which serves as both putative abscisic acid (ABA) receptor (ABAR) in Arabidopsis thaliana and positive regulator of strawberry fruit ripening. However, whether SigE influences strawberry fruit ripening has not been determined. We used RNA sequencing and a qPCR to confirm that the expression of the strawberry SigE gene ( FaSigE ) is rapidly upregulated in fruits turning red, suggesting FaSigE might mediate strawberry fruit ripening. Silencing FaSigE by intron-spliced hairpin RNA-mediated RNA interference significantly inhibited fruit ripening. This observation was confirmed by analyses of fruit firmness, soluble sugar, ABA, and anthocyanin contents, as well as transcript levels of genes related to fruit ripening and ABA signaling. Interestingly, a firefly luciferase complementation assay revealed that FaSigE can interact with FaABAR, while an in vitro fruit disc incubation test indicated ABA induces FaSigE expression. Moreover, a surface plasmon resonance assay proved that FaABAR produced in yeast cells can bind to ABA, with a binding dissociation constant of 50 µM. In conclusion, FaSigE can interact with FaABAR and positively regulates strawberry fruit ripening via ABA.
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Claims 5
Claim · model-written summary
FaSigE expression rose rapidly in fruits turning red.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Organ / stage
- fruits turning rede0
- Gene
- FaSigEe0
- Outcome
- expression of the strawberry SigE genee0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“the expression of the strawberry SigE gene ( FaSigE ) is rapidly upregulated in fruits turning red”
Claim · model-written summary
Silencing FaSigE significantly inhibited strawberry fruit ripening.
- Taxon
- strawberryFragaria ×ananassat0
- Treatment
- Silencing FaSigE by intron-spliced hairpin RNA-mediated RNA interferencee0
- Gene
- FaSigEe0
- Outcome
- fruit ripeninge0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing FaSigE by intron-spliced hairpin RNA-mediated RNA interference significantly inhibited fruit ripening”
t0“strawberry fruit ripening”
Claim · model-written summary
FaSigE interacted with FaABAR in a luciferase complementation assay.
- Taxon
- strawberryFragaria ×ananassat0
- Gene
- FaSigEe0
- Outcome
- interact with FaABARe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a firefly luciferase complementation assay revealed that FaSigE can interact with FaABAR”
t0“strawberry fruit ripening”
Claim · model-written summary
ABA induced FaSigE expression in a fruit disc assay.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- fruit disce0
- Treatment
- ABAe0
- Gene
- FaSigEe0
- Outcome
- FaSigE expressione0
- Direction
- inducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an in vitro fruit disc incubation test indicated ABA induces FaSigE expression”
t0“strawberry fruit ripening”
Claim · model-written summary
Yeast-produced FaABAR bound ABA with a dissociation constant of 50 µM.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaABAR produced in yeast cells can bind to ABA, with a binding dissociation constant of 50 µM”
t0“strawberry fruit ripening”
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