Abstract
BACKGROUND: MYB transcription factors (TFs) are one of the largest families of TFs in higher plants and are involved in diverse biological, functional, and structural processes. Previously, very few functional validation studies on R2R3 MYB have been conducted in cotton in response to abiotic stresses. In the current study, GaMYB85, a cotton R2R3 MYB TF, was ectopically expressed in Arabidopsis thaliana (Col-0) and was functionally characterized by overexpression in transgenic plants. RESULTS: The in-silico analysis of GaMYB85 shows the presence of a SANT domain with a conserved R2R3 MYB imperfect repeat. The GaMYB85 protein has a 257-amino acid sequence, a molecular weight of 24.91 kD, and an isoelectric point of 5.58. Arabidopsis plants overexpressing GaMYB85 exhibited a higher seed germination rate in response to mannitol and salt stress, and higher drought avoidance efficiency than wild-type plants upon water deprivation. These plants had notably higher levels of free proline and chlorophyll with subsequent lower water loss rates and higher relative water content. Germination of GaMYB85 transgenics was more sensitive to abscisic acid (ABA) and extremely liable to ABA-induced inhibition of primary root elongation. Moreover, when subjected to treatment with different concentrations of ABA, transgenic plants with ectopically expressed GaMYB85 showed reduced stomatal density, with greater stomatal size and lower stomatal opening rates than those in wild-type plants. Ectopic expression of GaMYB85 led to enhanced transcript levels of stress-related marker genes such as RD22, ADH1, RD29A, P5CS, and ABI5. CONCLUSIONS: Our results indicate previously unknown roles of GaMYB85, showing that it confers good drought, salt, and freezing tolerance, most probably via an ABA-induced pathway. These findings can potentially be exploited to develop improved abiotic stress tolerance in cotton plants.
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Claims 16
Claim · model-written summary
GaMYB85 had a SANT domain and conserved R2R3 repeat.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Gene
- GaMYB85e0
- Outcome
- SANT domain with a conserved R2R3 MYB imperfect repeate0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GaMYB85 shows the presence of a SANT domain with a conserved R2R3 MYB imperfect repeat”
e1“Arabidopsis thaliana (Col-0)”
Claim · model-written summary
GaMYB85 protein had 257 amino acids.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- GaMYB85e0
- Outcome
- amino acid sequencee0
- Value
- 257-amino acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GaMYB85 protein has a 257-amino acid sequence, a molecular weight of 24.91 kD, and an isoelectric point of 5.58”
e1“Arabidopsis thaliana (Col-0)”
Claim · model-written summary
GaMYB85 protein molecular weight was 24.91 kD.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- GaMYB85e0
- Outcome
- molecular weighte0
- Value
- 24.91 kDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GaMYB85 protein has a 257-amino acid sequence, a molecular weight of 24.91 kD, and an isoelectric point of 5.58”
e1“Arabidopsis thaliana (Col-0)”
Claim · model-written summary
GaMYB85 protein isoelectric point was 5.58.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- GaMYB85e0
- Outcome
- isoelectric pointe0
- Value
- 5.58e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GaMYB85 protein has a 257-amino acid sequence, a molecular weight of 24.91 kD, and an isoelectric point of 5.58”
e1“Arabidopsis thaliana (Col-0)”
Claim · model-written summary
GaMYB85 plants germinated at higher rates under mannitol and salt stress than wild type.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpressing GaMYB85e0
- Gene
- GaMYB85e0
- Outcome
- seed germination ratee0
- Direction
- highere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis plants overexpressing GaMYB85 exhibited a higher seed germination rate in response to mannitol and salt stress, and higher drought avoidance efficiency than wild-type plants upon water deprivation”
Claim · model-written summary
GaMYB85 plants had higher drought avoidance than wild type during water deprivation.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing GaMYB85e2
- Outcome
- drought avoidance efficiencye0
- Direction
- highere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher drought avoidance efficiency than wild-type plants upon water deprivation”
t0“Arabidopsis”
e2“overexpressing GaMYB85”
Claim · model-written summary
GaMYB85 plants had higher free proline and chlorophyll levels.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing GaMYB85e2
- Outcome
- levels of free proline and chlorophylle0
- Direction
- highere0
- Comparator
- wild-type plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These plants had notably higher levels of free proline and chlorophyll”
t0“Arabidopsis”
e2“overexpressing GaMYB85”
e3“higher drought avoidance efficiency than wild-type plants upon water deprivation”
Claim · model-written summary
GaMYB85 plants had lower water loss and higher relative water content.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing GaMYB85e2
- Outcome
- water loss ratese0
- Direction
- lowere0
- Comparator
- wild-type plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with subsequent lower water loss rates and higher relative water content”
t0“Arabidopsis”
e2“overexpressing GaMYB85”
e3“higher drought avoidance efficiency than wild-type plants upon water deprivation”
Claim · model-written summary
GaMYB85 plants had higher relative water content.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing GaMYB85e2
- Outcome
- relative water contente0
- Direction
- highere0
- Comparator
- wild-type plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher relative water content”
t0“Arabidopsis”
e2“overexpressing GaMYB85”
e3“higher drought avoidance efficiency than wild-type plants upon water deprivation”
Claim · model-written summary
GaMYB85 germination was more sensitive to ABA.
- Taxon
- ArabidopsisArabidopsist0
- Material
- GaMYB85 transgenicse0
- Treatment
- abscisic acid (ABA)e0
- Outcome
- Germinatione0
- Direction
- more sensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Germination of GaMYB85 transgenics was more sensitive to abscisic acid (ABA)”
t0“Arabidopsis”
Claim · model-written summary
GaMYB85 plants showed strong ABA inhibition of primary root elongation.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ABAe0
- Outcome
- primary root elongatione0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extremely liable to ABA-induced inhibition of primary root elongation”
t0“Arabidopsis”
Claim · model-written summary
Under ABA, GaMYB85 plants had lower stomatal density than wild type.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ectopically expressed GaMYB85e0
- Outcome
- stomatal densitye0
- Direction
- reducede0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic plants with ectopically expressed GaMYB85 showed reduced stomatal density, with greater stomatal size and lower stomatal opening rates than those in wild-type plants”
t0“Arabidopsis”
Claim · model-written summary
Under ABA, GaMYB85 plants had greater stomatal size.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ectopically expressed GaMYB85e0
- Outcome
- stomatal sizee0
- Direction
- greatere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic plants with ectopically expressed GaMYB85 showed reduced stomatal density, with greater stomatal size and lower stomatal opening rates than those in wild-type plants”
t0“Arabidopsis”
Claim · model-written summary
Under ABA, GaMYB85 plants had lower stomatal opening rates.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ectopically expressed GaMYB85e0
- Outcome
- stomatal opening ratese0
- Direction
- lowere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic plants with ectopically expressed GaMYB85 showed reduced stomatal density, with greater stomatal size and lower stomatal opening rates than those in wild-type plants”
t0“Arabidopsis”
Claim · model-written summary
GaMYB85 expression enhanced transcription of five stress marker genes.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Ectopic expression of GaMYB85e0
- Gene
- RD22, ADH1, RD29A, P5CS, and ABI5e0
- Outcome
- transcript levels of stress-related marker genese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of GaMYB85 led to enhanced transcript levels of stress-related marker genes such as RD22, ADH1, RD29A, P5CS, and ABI5”
t0“Arabidopsis”
Claim · model-written summary
Authors concluded GaMYB85 confers drought, salt and freezing tolerance, probably through ABA signaling.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- GaMYB85e0
- Outcome
- drought, salt, and freezing tolerancee0
- Qualifier
- most probably via an ABA-induced pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GaMYB85, showing that it confers good drought, salt, and freezing tolerance, most probably via an ABA-induced pathway”
t0“Arabidopsis”
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