Abstract
In vitro regeneration of pea explants (Pisum sativum L. var. ‘Lincon’) was done in 49 different combinations and concentrations of BAP, BAP and NAA, BAP and IBA, TDZ, TDZ and Adenine for shoot regeneration from hypocotyl, root, leaf and cotyledonary node. High frequency shoot regeneration was obtained in hypocotyl (81.43%), root(83.53%) and cotyledonary node(72.76%) on MS medium supplemented with 4.50 mg/l BAP and 1.86mg/l NAA, 2.00mg/l TDZ and4.50 mg/l BAP and 1.86mg/l NAA respectively. No shoot regeneration was obtained from leaf explants on any of the combination used. Shoot elongation was observed on the same medium used for shoot regeneration respectively.MS medium supplemented with 0.20 mg/l IBA was found best for root regeneration from in vitro raised shoots. The plantlets were able to regenerate within 6-7 weeks. The regenerated plantlets were acclimatized in pre-sterilized cocopeat.
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Claims 8
Claim · model-written summary
Hypocotyl explants regenerated shoots at 81.43% on BAP and NAA medium.
- Taxon
- pea
Lathyrus oleraceust0 - Organ / stage
- hypocotyle0
- Treatment
- 4.50 mg/l BAP and 1.86mg/l NAAe0
- Outcome
- shoot regeneratione0
- Direction
- High frequencye0
- Value
- 81.43%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High frequency shoot regeneration was obtained in hypocotyl (81.43%), root(83.53%) and cotyledonary node(72.76%) on MS medium supplemented with 4.50 mg/l BAP and 1.86mg/l NAA, 2.00mg/l TDZ and4.50 mg/l BAP and 1.86mg/l NAA respectively”
t0“pea (Pisum sativum L.)”
Claim · model-written summary
Root explants regenerated shoots at 83.53% on TDZ medium.
- Taxon
- peaLathyrus oleraceust0
- Organ / stage
- roote0
- Treatment
- 2.00mg/l TDZe0
- Outcome
- shoot regeneratione0
- Direction
- High frequencye0
- Value
- 83.53%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High frequency shoot regeneration was obtained in hypocotyl (81.43%), root(83.53%) and cotyledonary node(72.76%) on MS medium supplemented with 4.50 mg/l BAP and 1.86mg/l NAA, 2.00mg/l TDZ and4.50 mg/l BAP and 1.86mg/l NAA respectively”
t0“pea (Pisum sativum L.)”
Claim · model-written summary
Cotyledonary nodes regenerated shoots at 72.76% on BAP and NAA medium.
- Taxon
- peaLathyrus oleraceust0
- Organ / stage
- cotyledonary nodee0
- Treatment
- 4.50 mg/l BAP and 1.86mg/l NAAe0
- Outcome
- shoot regeneratione0
- Direction
- High frequencye0
- Value
- 72.76%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High frequency shoot regeneration was obtained in hypocotyl (81.43%), root(83.53%) and cotyledonary node(72.76%) on MS medium supplemented with 4.50 mg/l BAP and 1.86mg/l NAA, 2.00mg/l TDZ and4.50 mg/l BAP and 1.86mg/l NAA respectively”
t0“pea (Pisum sativum L.)”
Claim · model-written summary
Leaf explants did not regenerate shoots on any combination.
- Taxon
- peaLathyrus oleraceust0
- Organ / stage
- leaf explantse0
- Outcome
- No shoot regeneratione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No shoot regeneration was obtained from leaf explants on any of the combination used.”
t0“pea (Pisum sativum L.)”
Claim · model-written summary
Shoots elongated on their regeneration media.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- same medium used for shoot regeneratione0
- Outcome
- Shoot elongatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot elongation was observed on the same medium used for shoot regeneration respectively.”
t0“pea (Pisum sativum L.)”
Claim · model-written summary
MS medium with IBA was best for root regeneration.
- Taxon
- peaLathyrus oleraceust0
- Organ / stage
- in vitro raised shootse0
- Treatment
- 0.20 mg/l IBAe0
- Outcome
- root regeneratione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 0.20 mg/l IBA was found best for root regeneration from in vitro raised shoots.”
t0“pea (Pisum sativum L.)”
Claim · model-written summary
Plantlets regenerated within 6-7 weeks.
- Taxon
- peaLathyrus oleraceust0
- Outcome
- plantlets were able to regeneratee0
- Value
- 6-7 weekse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plantlets were able to regenerate within 6-7 weeks.”
t0“pea (Pisum sativum L.)”
Claim · model-written summary
Regenerated plantlets were acclimatized in pre-sterilized cocopeat.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- pre-sterilized cocopeate0
- Outcome
- acclimatizede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regenerated plantlets were acclimatized in pre-sterilized cocopeat.”
t0“pea (Pisum sativum L.)”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.