Abstract
Pit iuba cowpea (Vigna unguiculata (L.) Walp.) seeds were sown in distilled water or in 100mM NaCl solution. This salinity level was sufficient to reduce seedling length in about 65.4%. The dif ferences in coydedonary αand β-galactosidases activities between control and salt treatment along germination and seedling establishment suggest that salinity delays enzyme solubilization/activation or enzyme turnover. The in vitro experiments have shown that 100mM NaCl added to the assay medium did not affect the activities of these enzymes, and gave support to the hypothesis that the in vivo effect of salinity should be on enzyme solubi l ization/activation or enzyme turnover. When cotyledonary α-galactosidase enriched fractions (F20-85) from quiescent and 5-days old seedlings from both control and salt treatments were subjected to gel filtration through Sephadex G-100 it was observed the presence of only one peak of act ivi ty corresponding to a molecular mass of ≅43.5kD, and that salinity did not induce qualitative changes in the enzyme. The possibility that under the experimental conditions used this enzyme is in monomeric form is discussed. Additional
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Claims 5
Claim · model-written summary
100mM NaCl reduced cowpea seedling length by about 65.4%.
- Taxon
- Vigna unguiculata (L.) Walp.
Vigna unguiculatae0 - Material
- Pit iuba cowpeae0
- Treatment
- 100mM NaCl solutione0
- Outcome
- seedling lengthe0
- Direction
- reducee0
- Value
- 65.4%e0
- Comparator
- distilled watere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pit iuba cowpea (Vigna unguiculata (L.) Walp.) seeds were sown in distilled water or in 100mM NaCl solution. This salinity level was sufficient to reduce seedling length in about 65.4%”
Claim · model-written summary
In vivo salinity altered cotyledonary galactosidase activities across germination and establishment, suggesting delayed solubilization, activation or turnover.
- Taxon
- Vigna unguiculata (L.) Walp.Vigna unguiculatat0
- Treatment
- salt treatmente0
- Gene
- αand β-galactosidasese0
- Outcome
- αand β-galactosidases activitiese0
- Qualifier
- suggest that salinity delays enzyme solubilization/activation or enzyme turnovere0
- Comparator
- controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The dif ferences in coydedonary αand β-galactosidases activities between control and salt treatment along germination and seedling establishment suggest that salinity delays enzyme solubilization/activation or enzyme turnover”
t0“Vigna unguiculata (L.) Walp.”
Claim · model-written summary
Adding 100mM NaCl to the assay medium did not affect galactosidase activities in vitro.
- Taxon
- Vigna unguiculata (L.) Walp.Vigna unguiculatat0
- Treatment
- 100mM NaCl added to the assay mediume0
- Outcome
- activities of these enzymese0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“100mM NaCl added to the assay medium did not affect the activities of these enzymes”
t0“Vigna unguiculata (L.) Walp.”
Claim · model-written summary
Gel filtration showed one α-galactosidase activity peak corresponding to about 43.5kD.
- Taxon
- Vigna unguiculata (L.) Walp.Vigna unguiculatat0
- Gene
- α-galactosidasee1
- Outcome
- only one peak of act ivi tye0
- Value
- ≅43.5kDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only one peak of act ivi ty corresponding to a molecular mass of ≅43.5kD”
e1“cotyledonary α-galactosidase enriched fractions”
t0“Vigna unguiculata (L.) Walp.”
Claim · model-written summary
Salinity did not induce qualitative changes in cotyledonary α-galactosidase.
- Taxon
- Vigna unguiculata (L.) Walp.Vigna unguiculatat0
- Treatment
- salinitye0
- Gene
- α-galactosidasee1
- Outcome
- qualitative changes in the enzymee0
- Direction
- did not inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salinity did not induce qualitative changes in the enzyme”
e1“cotyledonary α-galactosidase enriched fractions”
t0“Vigna unguiculata (L.) Walp.”
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