Paper · 1983 · Physiologia Plantarum

Effects of NaCl salinity in vivo and in vitro on ribonuclease activity of Vigna unguiculata cotyledons during germination

Eneas Gomes Filho; José Tarquínio Prisco; Francisco de Assis de Paiva Campos; et al.

7
claims
14
verbatim quotes
35
citations
1
research areas
—
Not open access

Abstract

Pitiuba bean [ Vigna unguiculata (L.) Walp.] seeds were sown in water or. in 0.1 M NaCl. Seedling growth and cotyledon nucleic acid mobilization were delayed by NaCl salinity. The differences in cotyledonary RNase activity between seeds sown in water and in NaCl solutions suggest that salinity delays the activation and/or de novo synthesis of the enzyme. Cotyledon extracts were subjected to gel filtration through Sephadex G‐100, and RNase activity measured. Only one cotyledonary RNase appeared during germination, and salinity did not induce any change in molecular weight of the enzyme. Salinity inhibited 45% of the specific activity of the RNase on the 5th day of the experimental period. The same salt concentration (0.1 M NaCl) added in vitro inhibited only 8 % of the specific activity of the enzyme. This difference may indicate that NaCl in vivo affects mainly the de novo synthesis of the RNase.

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Claims 7

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

NaCl delayed seedling growth and cotyledon nucleic acid mobilization.

Taxon
Vigna unguiculataVigna unguiculatat0
Treatment
NaCl salinitye0
Outcome
Seedling growth and cotyledon nucleic acid mobilizatione0
Direction
delayede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Seedling growth and cotyledon nucleic acid mobilization were delayed by NaCl salinity.”

✓ located verbatim in the source · abstract, characters 89–175 · show in text ↑
t0

“Vigna unguiculata”

✓ located verbatim in the source · title, characters 74–91 · show in text ↑
Mechanismauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

Salinity appeared to delay RNase activation or new synthesis.

Taxon
Vigna unguiculataVigna unguiculatat0
Treatment
salinitye0
Outcome
activation and/or de novo synthesis of the enzymee0
Qualifier
de novo synthesise0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“salinity delays the activation and/or de novo synthesis of the enzyme.”

✓ located verbatim in the source · abstract, characters 286–356 · show in text ↑
t0

“Vigna unguiculata”

✓ located verbatim in the source · title, characters 74–91 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Only one cotyledonary RNase appeared during germination.

Taxon
Vigna unguiculataVigna unguiculatat0
Gene
RNasee0
Outcome
one cotyledonary RNasee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Only one cotyledonary RNase appeared during germination”

✓ located verbatim in the source · abstract, characters 462–517 · show in text ↑
t0

“Vigna unguiculata”

✓ located verbatim in the source · title, characters 74–91 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Salinity did not alter RNase molecular weight.

Taxon
Vigna unguiculataVigna unguiculatat0
Treatment
salinitye0
Outcome
molecular weight of the enzymee0
Direction
did not induce any changee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“salinity did not induce any change in molecular weight of the enzyme.”

✓ located verbatim in the source · abstract, characters 523–592 · show in text ↑
t0

“Vigna unguiculata”

✓ located verbatim in the source · title, characters 74–91 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

In vivo salinity inhibited RNase specific activity by 45% on day five.

Taxon
Vigna unguiculataVigna unguiculatat0
Treatment
Salinitye0
Gene
RNasee0
Outcome
specific activity of the RNasee0
Direction
inhibitede0
Value
45%e0
Qualifier
5th daye0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Salinity inhibited 45% of the specific activity of the RNase on the 5th day of the experimental period.”

✓ located verbatim in the source · abstract, characters 593–696 · show in text ↑
t0

“Vigna unguiculata”

✓ located verbatim in the source · title, characters 74–91 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

In vitro 0.1 M NaCl inhibited RNase specific activity by 8%.

Taxon
Vigna unguiculataVigna unguiculatat0
Treatment
0.1 M NaCle0
Outcome
specific activity of the enzymee0
Direction
inhibitede0
Value
8 %e0
Setting
in vitroe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The same salt concentration (0.1 M NaCl) added in vitro inhibited only 8 % of the specific activity of the enzyme.”

✓ located verbatim in the source · abstract, characters 697–811 · show in text ↑
t0

“Vigna unguiculata”

✓ located verbatim in the source · title, characters 74–91 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The in vivo versus in vitro difference may reflect an effect on RNase synthesis.

Taxon
Vigna unguiculataVigna unguiculatat0
Treatment
NaCl in vivoe0
Gene
RNasee0
Outcome
de novo synthesis of the RNasee0
Qualifier
may indicatee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“This difference may indicate that NaCl in vivo affects mainly the de novo synthesis of the RNase.”

✓ located verbatim in the source · abstract, characters 812–909 · show in text ↑
t0

“Vigna unguiculata”

✓ located verbatim in the source · title, characters 74–91 · show in text ↑

sodium chloride · growth · Vigna unguiculata (cowpea)Results differ

22 papers: 6 positive, 16 negative or no effect

15 papers in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. negative
  3. positive

18 papers more in this group

See all 22 papers →

sodium chloride · physiology: enzyme activity · Vigna unguiculata (cowpea)Positive and negative results

4 papers: 1 positive, 3 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. negative
See all 4 papers →

salinity · growth · Vigna unguiculata (cowpea)Results differ

63 papers: 6 positive, 57 negative or no effect

56 papers in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. negative
  3. positive

59 papers more in this group

See all 63 papers →

salinity · physiology: enzyme activity · Vigna unguiculata (cowpea)Results agree

7 papers: 0 positive, 7 negative or no effect

6 papers in the same direction as this paper

  1. no effect
  2. negative
  3. negative

3 papers more in this group

See all 7 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.