Abstract
Plant architecture is a major target of rice (Oryza sativa) breeding and selection, but the underlying regulatory networks remain unclear. Here, we overexpressed an OsNAC2 mutant (OErN) that cannot be cleaved by the miRNA miR164b. OErN plants had better plant architecture and longer panicles, and produced more grains. The parental line averaged 12.2 primary and 31.5 secondary branches in the main panicles; two OErN lines averaged 15.0 and 15.2 primary, and 41.5 and 44.3 secondary branches. In large-scale field trials, OErN plants produced at least 58.62% more total grain (by weight) compared with the parental line. They also had more large and small vascular bundles in the stem internodes and leaves. Overexpression of miR164b or down-regulation of OsNAC2 led to decreased panicle length and grain yield in the main panicle. The OErN plants showed significant up-regulation of the grain number and plant architecture-related genes IPA1 and DEP1. A survey of >3000 rice varieties found no natural mutations in the miR164b-binding site of OsNAC2. OErN increased yield in Nipponbare and the commonly grown Yangyujing 3 cultivars. In summary, we identified an efficient new strategy to increase rice yield substantially and improve plant architecture through overexpression of OsmiR164b-resistant OsNAC2.
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Claims 11
Claim · model-written summary
OErN plants had longer panicles and more grains.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OErN plants had better plant architecture and longer panicles, and produced more grains.”
t0“rice”
Claim · model-written summary
OErN lines had 15.0 and 15.2 primary panicle branches versus 12.2 in the parental line.
- Taxon
- rice
Oryza sativat0 - Material
- OErNe0
- Treatment
- OErNe0
- Outcome
- primarye0
- Direction
- higherinferrede0
- Value
- 15.0 and 15.2e0
- Qualifier
- 12.2e0
- Comparator
- parental linee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The parental line averaged 12.2 primary and 31.5 secondary branches in the main panicles; two OErN lines averaged 15.0 and 15.2 primary, and 41.5 and 44.3 secondary branches.”
t0“rice”
Claim · model-written summary
OErN lines had 41.5 and 44.3 secondary panicle branches versus 31.5 in the parental line.
- Taxon
- riceOryza sativat0
- Material
- OErNe0
- Treatment
- OErNe0
- Outcome
- secondary branchese0
- Direction
- higherinferrede0
- Value
- 41.5 and 44.3e0
- Qualifier
- 31.5e0
- Comparator
- parental linee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The parental line averaged 12.2 primary and 31.5 secondary branches in the main panicles; two OErN lines averaged 15.0 and 15.2 primary, and 41.5 and 44.3 secondary branches.”
t0“rice”
Claim · model-written summary
OErN plants produced at least 58.62% more total grain by weight than the parental line in field trials.
- Taxon
- riceOryza sativat0
- Treatment
- OErNe0
- Outcome
- total grain (by weight)e0
- Direction
- moree0
- Value
- 58.62%e0
- Comparator
- parental linee0
- Setting
- field trialse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In large-scale field trials, OErN plants produced at least 58.62% more total grain (by weight) compared with the parental line.”
t0“rice”
Claim · model-written summary
OErN plants had more large and small vascular bundles in stem internodes and leaves.
- Taxon
- riceOryza sativat0
- Organ / stage
- stem internodes and leavese0
- Treatment
- Overexpression of miR164b-resistant OsNAC2t0
- Outcome
- vascular bundlese0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They also had more large and small vascular bundles in the stem internodes and leaves.”
t0“Overexpression of miR164b-resistant OsNAC2 improves plant architecture and grain yield in rice”
Claim · model-written summary
Overexpression of miR164b decreased panicle length and grain yield in the main panicle.
- Taxon
- riceOryza sativat0
- Treatment
- Overexpression of miR164be0
- Gene
- miR164be0
- Outcome
- panicle length and grain yielde0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of miR164b or down-regulation of OsNAC2 led to decreased panicle length and grain yield in the main panicle.”
t0“rice”
Claim · model-written summary
Down-regulation of OsNAC2 decreased panicle length and grain yield in the main panicle.
- Taxon
- riceOryza sativat0
- Treatment
- down-regulation of OsNAC2e0
- Gene
- OsNAC2e0
- Outcome
- panicle length and grain yielde0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of miR164b or down-regulation of OsNAC2 led to decreased panicle length and grain yield in the main panicle.”
t0“rice”
Claim · model-written summary
OErN plants significantly up-regulated IPA1 and DEP1.
- Taxon
- riceOryza sativat0
- Treatment
- OErNe0
- Gene
- IPA1 and DEP1e0
- Outcome
- up-regulatione0
- Direction
- up-regulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The OErN plants showed significant up-regulation of the grain number and plant architecture-related genes IPA1 and DEP1.”
t0“rice”
Claim · model-written summary
No natural mutation was found in the miR164b-binding site of OsNAC2 among more than 3000 rice varieties.
- Taxon
- riceOryza sativae0
- Gene
- OsNAC2e0
- Outcome
- no natural mutationse0
- Value
- >3000e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A survey of >3000 rice varieties found no natural mutations in the miR164b-binding site of OsNAC2.”
Claim · model-written summary
OErN increased yield in Nipponbare.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OErN increased yield in Nipponbare and the commonly grown Yangyujing 3 cultivars.”
t0“rice”
Claim · model-written summary
OErN increased yield in Yangyujing 3.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OErN increased yield in Nipponbare and the commonly grown Yangyujing 3 cultivars.”
t0“rice”
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See all 134 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.