Abstract
Sodium (Na+) accumulation in the cytosol will result in ion homeostasis imbalance and toxicity of transpiring leaves. Studies of salinity tolerance in the diploid wheat ancestor Triticum monococcum showed that HKT1;5-like gene was a major gene in the QTL for salt tolerance, named Nax2. In the present study, we were interested in investigating the molecular mechanisms underpinning the role of the HKT1;5 gene in salt tolerance in barley (Hordeum vulgare). A USDA mini-core collection of 2671 barley lines, part of a field trial was screened for salinity tolerance, and a Genome Wide Association Study (GWAS) was performed. Our results showed important SNPs that are correlated with salt tolerance that mapped to a region where HKT1;5 ion transporter located on chromosome 4. Furthermore, sodium (Na+) and potassium (K+) content analysis revealed that tolerant lines accumulate more sodium in roots and leaf sheaths, than in the sensitive ones. In contrast, sodium concentration was reduced in leaf blades of the tolerant lines under salt stress. In the absence of NaCl, the concentration of Na+ and K+ were the same in the roots, leaf sheaths and leaf blades between the tolerant and the sensitive lines. In order to study the molecular mechanism behind that, alleles of the HKT1;5 gene from 5 tolerant and 5 sensitive barley lines were cloned and sequenced. Sequence analysis did not show the presence of any polymorphism that distinguishes between the tolerant and sensitive alleles. Our real-time RT-PCR experiments, showed that the expression of HKT1;5 gene in roots of the tolerant line was significantly induced after challenging the plants with salt stress. In contrast, in leaf sheaths the expression was decreased after salt treatment. In sensitive lines, there was no difference in the expression of HKT1;5 gene in leaf sheath under control and saline conditions, while a slight increase in the expression was observed in roots after salt treatment. These results provide stronger evidence that HKT1;5 gene in barley play a key role in withdrawing Na+ from the xylem and therefore reducing its transport to leaves. Given all that, these data support the hypothesis that HKT1;5 gene is responsible for
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Claims 12
Claim · model-written summary
SNPs correlated with salt tolerance mapped near HKT1;5 on chromosome 4.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Gene
- HKT1;5e0
- Outcome
- salt tolerancee0
- Qualifier
- chromosome 4e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“important SNPs that are correlated with salt tolerance that mapped to a region where HKT1;5 ion transporter located on chromosome 4”
t0“Barley”
Claim · model-written summary
Tolerant lines accumulated more sodium in roots than sensitive lines.
- Taxon
- barleyHordeum vulgaret0
- Material
- tolerant linese0
- Organ / stage
- rootse0
- Outcome
- sodiume0
- Direction
- moree0
- Comparator
- sensitive onese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tolerant lines accumulate more sodium in roots and leaf sheaths, than in the sensitive ones”
t0“Barley”
Claim · model-written summary
Tolerant lines accumulated more sodium in leaf sheaths than sensitive lines.
- Taxon
- barleyHordeum vulgaret0
- Material
- tolerant linese0
- Organ / stage
- leaf sheathse0
- Outcome
- sodiume0
- Direction
- moree0
- Comparator
- sensitive onese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tolerant lines accumulate more sodium in roots and leaf sheaths, than in the sensitive ones”
t0“Barley”
Claim · model-written summary
Tolerant lines had reduced sodium concentration in leaf blades under salt stress.
- Taxon
- barleyHordeum vulgaret0
- Material
- tolerant linese0
- Organ / stage
- leaf bladese0
- Treatment
- salt stresse0
- Outcome
- sodium concentratione0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sodium concentration was reduced in leaf blades of the tolerant lines under salt stress”
t0“Barley”
Claim · model-written summary
Without NaCl, sodium concentrations were the same between tolerant and sensitive lines across the sampled organs.
- Taxon
- barleyHordeum vulgaret0
- Material
- tolerante0
- Outcome
- concentration of Na+e0
- Direction
- the samee0
- Qualifier
- In the absence of NaCle0
- Comparator
- sensitive linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the absence of NaCl, the concentration of Na+ and K+ were the same in the roots, leaf sheaths and leaf blades between the tolerant and the sensitive lines.”
t0“Barley”
Claim · model-written summary
Without NaCl, potassium concentrations were the same between tolerant and sensitive lines across the sampled organs.
- Taxon
- barleyHordeum vulgaret0
- Material
- tolerante0
- Outcome
- K+e0
- Direction
- the samee0
- Qualifier
- In the absence of NaCle0
- Comparator
- sensitive linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the absence of NaCl, the concentration of Na+ and K+ were the same in the roots, leaf sheaths and leaf blades between the tolerant and the sensitive lines.”
t0“Barley”
Claim · model-written summary
HKT1;5 sequence showed no polymorphism distinguishing tolerant and sensitive alleles.
- Taxon
- barleyHordeum vulgaret0
- Gene
- HKT1;5t0
- Outcome
- any polymorphisme0
- Direction
- did not showe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequence analysis did not show the presence of any polymorphism that distinguishes between the tolerant and sensitive alleles.”
t0“HKT1;5 Gene in Barley”
Claim · model-written summary
Salt stress significantly induced root HKT1;5 expression in the tolerant line.
- Taxon
- barleyHordeum vulgaret0
- Material
- tolerant linee0
- Organ / stage
- rootse0
- Treatment
- salt stresse0
- Gene
- HKT1;5e0
- Outcome
- expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of HKT1;5 gene in roots of the tolerant line was significantly induced after challenging the plants with salt stress”
t0“Barley”
Claim · model-written summary
Salt treatment decreased HKT1;5 expression in leaf sheaths of the tolerant line.
- Taxon
- barleyHordeum vulgaret0
- Organ / stage
- leaf sheathse0
- Treatment
- salt treatmente0
- Gene
- HKT1;5t0
- Outcome
- expressione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In contrast, in leaf sheaths the expression was decreased after salt treatment.”
t0“HKT1;5 Gene in Barley”
Claim · model-written summary
Sensitive lines showed no difference in leaf sheath HKT1;5 expression under control and saline conditions.
- Taxon
- barleyHordeum vulgaret0
- Material
- sensitive linese0
- Organ / stage
- leaf sheathe0
- Gene
- HKT1;5e0
- Outcome
- expressione0
- Direction
- no differencee0
- Comparator
- control and saline conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In sensitive lines, there was no difference in the expression of HKT1;5 gene in leaf sheath under control and saline conditions”
t0“Barley”
Claim · model-written summary
Sensitive lines showed a slight increase in root HKT1;5 expression after salt treatment.
- Taxon
- barleyHordeum vulgaret0
- Material
- sensitivee1
- Organ / stage
- rootse0
- Treatment
- salt treatmente0
- Gene
- HKT1;5t0
- Outcome
- expressione0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a slight increase in the expression was observed in roots after salt treatment”
t0“HKT1;5 Gene in Barley”
e1“In sensitive lines”
Claim · model-written summary
The authors interpret HKT1;5 as withdrawing Na+ from xylem and reducing transport to leaves.
- Taxon
- barleyHordeum vulgaree0
- Gene
- HKT1;5e0
- Compound
- Na+e0
- Outcome
- reducing its transport to leavese0
- Direction
- reducinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HKT1;5 gene in barley play a key role in withdrawing Na+ from the xylem and therefore reducing its transport to leaves”
The same questions in other papers
salinity · molecular: expression · Hordeum vulgare (barley)Positive and negative results
10 papers in the same direction as this paper · 10 in the opposite direction
- negative
- no effect
- no effect
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.