Abstract
We cloned a cDNA encoding Hordeum vulgare Proline Transporter (HvProT) from salt-stressed barley roots by differential display. HvProT was 2,161 bp long and had an open reading frame encoding 450 amino acids. The deduced amino acid sequence of HvProT was similar to those of proline transporter proteins of rice (65.7%), Arabidopsis (57.7%) and tomato (42.0%). Northern blot analysis showed that the transcript level of HvProT was induced in roots at 30 min after 200 mM NaCl treatment and its peak was observed at 3 h. However, the transcript level was very low in leaves and did not increase by salt stress. The expression level of Delta(1)-pyrroline-5-carboxylate synthetase (P5CS), encoding a key enzyme of proline synthesis, was induced later than HvProT by salt stress. A transport assay using a yeast with mutation in proline uptake revealed that HvProT was a transporter with high affinity for L-proline (K(m) = 25 microM). HvProT was found to be a unique transporter with high affinity for L-proline. Since its transport activity was dependent on the pH gradient, HvProT was suggested to be a H(+)/amino acid symporter. In situ hybridization analysis showed that the HvProT mRNA was strongly expressed in root cap cells under salt stress. HvProT might play an important role in the transport of proline to root tip region urgently upon salt stress.
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Claims 8
Claim · model-written summary
A barley HvProT cDNA was cloned from salt-stressed roots.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Gene
- HvProTe0
- Outcome
- cDNA encoding Hordeum vulgare Proline Transporter (HvProT)e0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“We cloned a cDNA encoding Hordeum vulgare Proline Transporter (HvProT) from salt-stressed barley roots by differential display.”
Claim · model-written summary
HvProT is 2,161 bp and encodes 450 amino acids.
- Taxon
- barleyHordeum vulgaret0
- Gene
- HvProTe0
- Outcome
- open reading frame encoding 450 amino acidse0
- Value
- 2,161 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HvProT was 2,161 bp long and had an open reading frame encoding 450 amino acids.”
t0“Barley”
Claim · model-written summary
HvProT root transcript rose after NaCl treatment at 30 min and peaked at 3 h.
- Taxon
- BarleyHordeum vulgaret0
- Organ / stage
- rootse0
- Treatment
- 200 mM NaCl treatmente0
- Gene
- HvProTe0
- Outcome
- transcript level of HvProTe0
- Direction
- inducede0
- Qualifier
- 30 mine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcript level of HvProT was induced in roots at 30 min after 200 mM NaCl treatment and its peak was observed at 3 h.”
t0“Barley”
Claim · model-written summary
Leaf HvProT transcript stayed very low and did not rise with salt stress.
- Taxon
- BarleyHordeum vulgaret0
- Organ / stage
- leavese0
- Treatment
- salt stresse0
- Outcome
- transcript levele0
- Direction
- did not increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcript level was very low in leaves and did not increase by salt stress.”
t0“Barley”
Claim · model-written summary
Salt induced P5CS expression later than HvProT.
- Taxon
- BarleyHordeum vulgaret0
- Gene
- Delta(1)-pyrroline-5-carboxylate synthetase (P5CS)e0
- Outcome
- expression levele0
- Direction
- induced later than HvProTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression level of Delta(1)-pyrroline-5-carboxylate synthetase (P5CS), encoding a key enzyme of proline synthesis, was induced later than HvProT by salt stress.”
t0“Barley”
Claim · model-written summary
HvProT transported L-proline with high affinity, Km 25 microM.
- Taxon
- BarleyHordeum vulgaret0
- Gene
- HvProTe0
- Outcome
- high affinity for L-prolinee0
- Value
- 25 microMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HvProT was a transporter with high affinity for L-proline (K(m) = 25 microM).”
t0“Barley”
Claim · model-written summary
HvProT transport depended on the pH gradient, suggesting a H(+)/amino acid symporter.
- Taxon
- BarleyHordeum vulgaret0
- Outcome
- H(+)/amino acid symportere0
- Qualifier
- dependent on the pH gradiente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Since its transport activity was dependent on the pH gradient, HvProT was suggested to be a H(+)/amino acid symporter.”
t0“Barley”
Claim · model-written summary
HvProT mRNA was strongly expressed in salt-stressed root cap cells.
- Taxon
- BarleyHordeum vulgaret0
- Organ / stage
- root cap cellse0
- Gene
- HvProTe0
- Outcome
- HvProT mRNAe0
- Qualifier
- under salt stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HvProT mRNA was strongly expressed in root cap cells under salt stress.”
t0“Barley”
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See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.