Abstract
Abstract NAC transcription factors are widespread in the plant kingdom and play essential roles in the transcriptional regulation of defense responses. In this study, we isolated a novel NAC transcription factor gene, TaNAC30 , from a cDNA library constructed from wheat ( Triticum aestivum ) plants inoculated with the stripe rust pathogen Puccinia striiformis f. sp. tritici ( Pst ). TaNAC30 contains a typical NAM domain and localizes to the nucleus. Yeast one‐hybrid assays revealed that TaNAC30 exhibits transcriptional activity and that its C‐terminus is necessary for the activation of transcription. Expression of TaNAC30 increased when host plants were infected with a virulent race (CYR31) of the rust fungus Pst . Silencing of TaNAC30 by virus‐induced gene silencing inhibited colonization of the virulent Pst isolate CYR31. Moreover, detailed histological analyses showed that silencing of TaNAC30 enhanced resistance to Pst by inducing a significant increase in the accumulation of H 2 O 2 . Finally, we overexpressed TaNAC30 in fission yeast and determined that cell viability was severely reduced in TaNAC30 ‐transformed cells grown on medium containing H 2 O 2 . These results suggest that TaNAC30 negatively regulates plant resistance in a compatible wheat‐ Pst interaction.
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Claims 9
Claim · model-written summary
TaNAC30 contains a NAM domain and localizes to the nucleus.
- Taxon
- wheat
Triticum aestivumt0 - Gene
- TaNAC30e0
- Outcome
- typical NAM domain and localizes to the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC30 contains a typical NAM domain and localizes to the nucleus.”
t0“A novel wheat NAC transcription factor”
Claim · model-written summary
TaNAC30 had transcriptional activity.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaNAC30e0
- Outcome
- transcriptional activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC30 exhibits transcriptional activity”
t0“A novel wheat NAC transcription factor”
Claim · model-written summary
The TaNAC30 C-terminus was necessary for transcription activation.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- activation of transcriptione0
- Qualifier
- C‐terminuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its C‐terminus is necessary for the activation of transcription.”
t0“A novel wheat NAC transcription factor”
Claim · model-written summary
TaNAC30 expression increased during infection by virulent CYR31.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- infected with a virulent race (CYR31) of the rust fungus Pste0
- Interactor
- Pste0pathogen
- Gene
- TaNAC30e0
- Outcome
- Expression of TaNAC30e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of TaNAC30 increased when host plants were infected with a virulent race (CYR31) of the rust fungus Pst .”
t0“A novel wheat NAC transcription factor”
Claim · model-written summary
TaNAC30 silencing inhibited colonization by CYR31.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Silencing of TaNAC30 by virus‐induced gene silencinge0
- Interactor
- Pste0pathogen
- Gene
- TaNAC30e0
- Outcome
- colonization of the virulent Pst isolate CYR31e0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of TaNAC30 by virus‐induced gene silencing inhibited colonization of the virulent Pst isolate CYR31.”
t0“A novel wheat NAC transcription factor”
Claim · model-written summary
TaNAC30 silencing enhanced resistance to Pst.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- silencing of TaNAC30e0
- Interactor
- Pste0pathogen
- Gene
- TaNAC30e0
- Outcome
- resistance to Pste0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of TaNAC30 enhanced resistance to Pst by inducing a significant increase in the accumulation of H 2 O 2 .”
t0“A novel wheat NAC transcription factor”
Claim · model-written summary
TaNAC30 silencing significantly increased H 2 O 2 accumulation.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- silencing of TaNAC30e0
- Interactor
- Pste0pathogen
- Gene
- TaNAC30e0
- Outcome
- accumulation of H 2 O 2e0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of TaNAC30 enhanced resistance to Pst by inducing a significant increase in the accumulation of H 2 O 2 .”
t0“A novel wheat NAC transcription factor”
Claim · model-written summary
TaNAC30 expression in fission yeast severely reduced cell viability on H 2 O 2 medium.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- TaNAC30 ‐transformede0
- Gene
- TaNAC30e0
- Outcome
- cell viabilitye0
- Direction
- severely reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell viability was severely reduced in TaNAC30 ‐transformed cells grown on medium containing H 2 O 2 .”
t0“A novel wheat NAC transcription factor”
Claim · model-written summary
The authors infer TaNAC30 negatively regulates wheat resistance to Pst.
- Taxon
- wheatTriticum aestivume0
- Interactor
- Pste0pathogen
- Gene
- TaNAC30e0
- Outcome
- plant resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaNAC30 negatively regulates plant resistance in a compatible wheat‐ Pst interaction.”
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