Abstract
Calcineurin B-like proteins (CBLs) act as Ca2+ sensors to activate specific protein kinases, namely CBL-interacting protein kinases (CIPKs). Recent research has demonstrated that the CBL-CIPK complex is not only required for abiotic stress signaling, but is also probably involved in biotic stress perception. However, the role of this complex in immune signaling, including pathogen perception, is unknown. In this study, we isolated one signaling component of the TaCBL-TaCIPK complex (TaCBL4-TaCIPK5) and characterized its role in the interaction between wheat (Triticum aestivum) and Puccinia striiformis f. sp. tritici (Pst, stripe rust fungus). Among all TaCBLs in wheat, TaCBL4 mRNA accumulation markedly increased after infection by Pst. Silencing of TaCBL4 resulted in enhanced susceptibility to avirulent Pst infection. In addition, screening determined that TaCIPK5 physically interacted with TaCBL4 in planta and positively contributed to wheat resistance to Pst. Moreover, the disease resistance phenotype of TaCBL4 and TaCIPK5 co-silenced plants was consistent with that of single-knockdown plants. The accumulation of reactive oxygen species (ROS) was significantly altered in all silenced plants during Pst infection. Together these findings demonstrate that the TaCBL4-TaCIPK5 complex positively modulates wheat resistance in a ROS-dependent manner, and provide new insights into the roles of CBL-CIPK in wheat.
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Claims 7
Claim · model-written summary
Infection by Pst markedly increased TaCBL4 mRNA accumulation in wheat.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- infection by Pste0
- Interactor
- Puccinia striiformis f. sp. tritici
Puccinia striiformis f. sp. triticie1pathogen - Gene
- TaCBL4e0
- Outcome
- mRNA accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among all TaCBLs in wheat, TaCBL4 mRNA accumulation markedly increased after infection by Pst”
e1“characterized its role in the interaction between wheat (Triticum aestivum) and Puccinia striiformis f. sp. tritici (Pst, stripe rust fungus)”
Claim · model-written summary
Silencing of TaCBL4 enhanced wheat susceptibility to avirulent Puccinia striiformis f. sp. tritici.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Silencing of TaCBL4e2
- Interactor
- Puccinia striiformis f. sp. triticiPuccinia striiformis f. sp. triticie1pathogen
- Gene
- TaCBL4e2
- Outcome
- susceptibilitye2
- Direction
- enhancede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“characterized its role in the interaction between wheat (Triticum aestivum) and Puccinia striiformis f. sp. tritici (Pst, stripe rust fungus)”
e2“Silencing of TaCBL4 resulted in enhanced susceptibility to avirulent Pst infection”
Claim · model-written summary
TaCIPK5 physically interacted with TaCBL4 in planta in wheat.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaCIPK5e3
- Outcome
- physically interactede3
- Qualifier
- with TaCBL4 in plantae3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“characterized its role in the interaction between wheat (Triticum aestivum) and Puccinia striiformis f. sp. tritici (Pst, stripe rust fungus)”
e3“TaCIPK5 physically interacted with TaCBL4 in planta”
Claim · model-written summary
TaCIPK5 positively contributed to wheat resistance to Puccinia striiformis f. sp. tritici.
- Taxon
- wheatTriticum aestivume1
- Interactor
- Puccinia striiformis f. sp. triticiPuccinia striiformis f. sp. triticie1pathogen
- Gene
- TaCIPK5e3
- Outcome
- wheat resistance to Pste4
- Direction
- positively contributede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“characterized its role in the interaction between wheat (Triticum aestivum) and Puccinia striiformis f. sp. tritici (Pst, stripe rust fungus)”
e3“TaCIPK5 physically interacted with TaCBL4 in planta”
e4“positively contributed to wheat resistance to Pst”
Claim · model-written summary
Co-silenced TaCBL4 and TaCIPK5 wheat plants had a disease resistance phenotype consistent with single-knockdown plants.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaCBL4 and TaCIPK5e5
- Outcome
- disease resistance phenotypee5
- Qualifier
- consistent with that of single-knockdown plantse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“characterized its role in the interaction between wheat (Triticum aestivum) and Puccinia striiformis f. sp. tritici (Pst, stripe rust fungus)”
e5“the disease resistance phenotype of TaCBL4 and TaCIPK5 co-silenced plants was consistent with that of single-knockdown plants”
Claim · model-written summary
Silenced wheat plants showed significantly altered reactive oxygen species accumulation during Pst infection.
- Taxon
- wheatTriticum aestivume1
- Treatment
- silenced plantse6
- Interactor
- Pste6pathogen
- Outcome
- accumulation of reactive oxygen species (ROS)e6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“characterized its role in the interaction between wheat (Triticum aestivum) and Puccinia striiformis f. sp. tritici (Pst, stripe rust fungus)”
e6“The accumulation of reactive oxygen species (ROS) was significantly altered in all silenced plants during Pst infection”
Claim · model-written summary
The TaCBL4-TaCIPK5 complex positively modulates wheat resistance in a ROS-dependent manner.
- Taxon
- wheatTriticum aestivume7
- Gene
- TaCBL4-TaCIPK5 complexe7
- Outcome
- wheat resistancee7
- Qualifier
- in a ROS-dependent mannere7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“the TaCBL4-TaCIPK5 complex positively modulates wheat resistance in a ROS-dependent manner”
The same questions in other papers
loss of function · Puccinia striiformis f. sp. tritici · Triticum aestivum (wheat)Positive and negative results
9 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.