Abstract
Drought stress is a limiting environmental factor that affects plant growth and development. The plant hormone abscisic acid (ABA) plays an important role in plant drought responses. Previous studies have indicated that ABA inhibits plasma membrane H+-ATPase (PM H+-ATPase) activity, and the decrease in PM H+-ATPase activity promotes stomatal closure under drought stress, thereby reducing water loss. However, the underlying molecular mechanisms are not well understood. In this study, we found that in Arabidopsis (Arabidopsis thaliana), ABA induces an N-ethylmaleimide-sensitive factor attachment protein receptor protein, namely, VESICLE-ASSOCIATED MEMBRANE PROTEIN 711 (VAMP711), to interact with the Arabidopsis PM H+-ATPases AHA1 and AHA2. The interaction occurs at their C-termini and inhibits PM H+-ATPase activity. Deletion of VAMP711 in Arabidopsis results in a higher PM H+-ATPase activity and slower stomatal closure in response to ABA and drought treatments. In addition, overexpression of VAMP711 partially rescues the drought-sensitive phenotype of ost2-2D, a mutation in AHA1 resulting in a constitutive activated PM H+-ATPase. Our results demonstrate that VAMP711 is involved in regulating ABA-mediated inhibition of PM H+-ATPase activity and stomatal closure in response to drought stress.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
ABA induces VAMP711 interaction with AHA1 and AHA2 in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- ABAe0
- Gene
- VESICLE-ASSOCIATED MEMBRANE PROTEIN 711 (VAMP711)e0
- Outcome
- interact with the Arabidopsis PM H+-ATPases AHA1 and AHA2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in Arabidopsis (Arabidopsis thaliana), ABA induces an N-ethylmaleimide-sensitive factor attachment protein receptor protein, namely, VESICLE-ASSOCIATED MEMBRANE PROTEIN 711 (VAMP711), to interact with the Arabidopsis PM H+-ATPases AHA1 and AHA2”
Claim · model-written summary
The VAMP711 interaction at the C-termini inhibits proton pump activity.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- PM H+-ATPasee0
- Outcome
- PM H+-ATPase activitye0
- Direction
- inhibitse0
- Qualifier
- C-terminie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction occurs at their C-termini and inhibits PM H+-ATPase activity”
e1“in Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
VAMP711 deletion raises proton pump activity.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Deletion of VAMP711e0
- Gene
- VAMP711e0
- Outcome
- PM H+-ATPase activitye0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Deletion of VAMP711 in Arabidopsis results in a higher PM H+-ATPase activity”
Claim · model-written summary
VAMP711 deletion slows stomatal closure under ABA and drought treatments.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Deletion of VAMP711e0
- Gene
- VAMP711e0
- Outcome
- stomatal closuree0
- Direction
- slowere0
- Qualifier
- ABA and drought treatmentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Deletion of VAMP711 in Arabidopsis results in a higher PM H+-ATPase activity and slower stomatal closure in response to ABA and drought treatments”
Claim · model-written summary
VAMP711 overexpression partially rescues the drought-sensitive ost2-2D phenotype.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- overexpression of VAMP711e0
- Gene
- VAMP711e0
- Outcome
- drought-sensitive phenotype of ost2-2De0
- Direction
- partially rescuese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of VAMP711 partially rescues the drought-sensitive phenotype of ost2-2D”
e1“in Arabidopsis (Arabidopsis thaliana)”
The same questions in other papers
loss of function · physiology: gas exchange · ArabidopsisPositive and negative results
19 papers in the same direction as this paper · 5 in the opposite direction
- negative
- positive
- positive
21 papers more in this group
See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.