Abstract
The root plays an important role in the responses of plants to stresses, but the detailed mechanisms of roots in stress responses are still obscure. The GDP-mannose pyrophosphate synthetase (GMPase) OsVTC1-3 is a key factor of ascorbic acid (AsA) synthesis in rice roots. The present study showed that the transcript of OsVTC1-3 was induced by salt stress in roots, but not in leaves. Inhibiting the expression of OsVTC1-3 by RNA interfering (RI) technology significantly impaired the tolerance of rice to salt stress. The roots of OsVTC1-3 RI plants rapidly produced more O2−, and later accumulated amounts of H2O2 under salt stress, indicating the impaired tolerance of OsVTC1-3 RI plants to salt stress due to the decreasing ability of scavenging reactive oxygen species (ROS). Moreover, exogenous AsA restored the salt tolerance of OsVTC1-3 RI plants, indicating that the AsA synthesis in rice roots is an important factor for the response of rice to salt stress. Further studies showed that the salt-induced AsA synthesis was limited in the roots of OsVTC1-3 RI plants. The above results showed that specifically regulating AsA synthesis to scavenge ROS in rice roots was one of important factors in enhancing the tolerance of rice to salt stress.
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Claims 7
Claim · model-written summary
OsVTC1-3 transcript was induced by salt stress in rice roots but not in leaves.
- Taxon
- rice
Oryza sativae1 - Organ / stage
- rootse0
- Treatment
- salt stresse0
- Gene
- OsVTC1-3e0
- Outcome
- transcript of OsVTC1-3e0
- Direction
- inducede0
- Comparator
- leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The present study showed that the transcript of OsVTC1-3 was induced by salt stress in roots, but not in leaves”
e1“Inhibiting the expression of OsVTC1-3 by RNA interfering (RI) technology significantly impaired the tolerance of rice to salt stress”
Claim · model-written summary
RNA interference knockdown of OsVTC1-3 significantly impaired rice tolerance to salt stress.
- Taxon
- riceOryza sativae1
- Treatment
- Inhibiting the expression of OsVTC1-3 by RNA interfering (RI) technologye1
- Gene
- OsVTC1-3e1
- Outcome
- tolerance of rice to salt stresse1
- Direction
- impairede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Inhibiting the expression of OsVTC1-3 by RNA interfering (RI) technology significantly impaired the tolerance of rice to salt stress”
Claim · model-written summary
Roots of OsVTC1-3 RI plants rapidly produced more O2− under salt stress.
- Taxon
- riceOryza sativae1
- Material
- OsVTC1-3 RI plantse2
- Organ / stage
- rootse2
- Treatment
- salt stresse2
- Outcome
- O2−e2
- Direction
- produced moree2
- Qualifier
- rapidlye2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Inhibiting the expression of OsVTC1-3 by RNA interfering (RI) technology significantly impaired the tolerance of rice to salt stress”
e2“The roots of OsVTC1-3 RI plants rapidly produced more O2−, and later accumulated amounts of H2O2 under salt stress”
Claim · model-written summary
Roots of OsVTC1-3 RI plants later accumulated H2O2 under salt stress.
- Taxon
- riceOryza sativae1
- Material
- OsVTC1-3 RI plantse2
- Organ / stage
- rootse2
- Treatment
- salt stresse2
- Outcome
- H2O2e2
- Direction
- accumulatede2
- Qualifier
- latere2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Inhibiting the expression of OsVTC1-3 by RNA interfering (RI) technology significantly impaired the tolerance of rice to salt stress”
e2“The roots of OsVTC1-3 RI plants rapidly produced more O2−, and later accumulated amounts of H2O2 under salt stress”
Claim · model-written summary
The impaired salt tolerance of OsVTC1-3 RI plants is attributed to a decreased ability to scavenge ROS.
- Taxon
- riceOryza sativae1
- Outcome
- tolerance of OsVTC1-3 RI plants to salt stresse3
- Qualifier
- decreasing ability of scavenging reactive oxygen species (ROS)e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Inhibiting the expression of OsVTC1-3 by RNA interfering (RI) technology significantly impaired the tolerance of rice to salt stress”
e3“indicating the impaired tolerance of OsVTC1-3 RI plants to salt stress due to the decreasing ability of scavenging reactive oxygen species (ROS)”
Claim · model-written summary
Exogenous AsA restored the salt tolerance of OsVTC1-3 RI plants.
- Taxon
- riceOryza sativae1
- Material
- OsVTC1-3 RI plantse4
- Treatment
- exogenous AsAe4
- Compound
- AsAe4
- Outcome
- salt tolerance of OsVTC1-3 RI plantse4
- Direction
- restorede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Inhibiting the expression of OsVTC1-3 by RNA interfering (RI) technology significantly impaired the tolerance of rice to salt stress”
e4“exogenous AsA restored the salt tolerance of OsVTC1-3 RI plants”
Claim · model-written summary
Salt-induced AsA synthesis was limited in the roots of OsVTC1-3 RI plants.
- Taxon
- riceOryza sativae1
- Material
- OsVTC1-3 RI plantse5
- Organ / stage
- rootse5
- Treatment
- salt stresse0
- Compound
- AsAe5
- Outcome
- salt-induced AsA synthesise5
- Direction
- limitede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The present study showed that the transcript of OsVTC1-3 was induced by salt stress in roots, but not in leaves”
e1“Inhibiting the expression of OsVTC1-3 by RNA interfering (RI) technology significantly impaired the tolerance of rice to salt stress”
e5“the salt-induced AsA synthesis was limited in the roots of OsVTC1-3 RI plants”
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