Abstract
• OsAAI1 is salt-sensitive, inhibited by NaCl but promoted by nitrate. • Salt tolerance and ROS scavenging enzyme activity of OsAAI1 were enhanced by nitrate. • Root scanning provided more insight into root growth and development. • osaai1 was more resistant to salt stress compared to OE 19. • High concentration of nitrate reduced salt damage to OE 19. • OsAAI1 plays a key role in rice salt stress response through the nitrate pathway. Salt stress limits plant growth and yield. Though nitrogen fertilizer can alleviate salt damage, the effects of salt and nitrate on the stress resistance gene OsAAI1 are unclear. This study examined the Salt stress sensitivity of OsAAI1 transgenic lines and nitrate's role. Results showed OsAAI1 expression decreased with Salt and increased with nitrate. Under salt stress, the mutant OsAAI1 ( osaai1 ) had significantly higher plant height, root length and number, and lower ROS accumulation than ZH 11, while OsAAI1 overexpression (OE 19) showed opposite trends. OE 19 also had lower antioxidant enzyme activities and higher MDA content. Analyses of topology, biomass distribution and connectivity of root scans after 30 and 50 days of salt stress treatment showed that osaai1 was able to sustain root growth and development under salt stress conditions, whereas OE 19 was more damaged. Exogenous salt stress tests confirmed these findings. Notably, nitrate application enhanced OsAAI1 is salt tolerance, improving root growth and increasing ROS scavenging enzyme activities. Under KNO₃ induction, high-concentration KNO₃ restores the root phenotype in OE 19. In conclusion, overexpression of OsAAI1 was more sensitive to salt, and OsAAI1 regulated ROS homeostasis through the nitrate pathway to enhance its tolerance to salt stress.
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Claims 12
Claim · model-written summary
Salt decreased OsAAI1 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results showed OsAAI1 expression decreased with Salt and increased with nitrate”
t0“rice root development”
Claim · model-written summary
Nitrate increased OsAAI1 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results showed OsAAI1 expression decreased with Salt and increased with nitrate”
t0“rice root development”
Claim · model-written summary
Under salt stress, osaai1 had significantly greater plant height, root length and root number than ZH 11.
- Taxon
- rice
Oryza sativat0 - Material
- osaai1e0
- Treatment
- salt stresse0
- Gene
- OsAAI1e0
- Outcome
- plant height, root length and numbere0
- Direction
- highere0
- Comparator
- ZH 11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under salt stress, the mutant OsAAI1 ( osaai1 ) had significantly higher plant height, root length and number, and lower ROS accumulation than ZH 11”
t0“rice root development”
Claim · model-written summary
Under salt stress, osaai1 accumulated less ROS than ZH 11.
- Taxon
- riceOryza sativat0
- Material
- osaai1e0
- Treatment
- salt stresse0
- Outcome
- ROS accumulatione0
- Direction
- lowere0
- Comparator
- ZH 11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under salt stress, the mutant OsAAI1 ( osaai1 ) had significantly higher plant height, root length and number, and lower ROS accumulation than ZH 11”
t0“rice root development”
Claim · model-written summary
OsAAI1 overexpression showed opposite growth and ROS trends to osaai1 under salt stress.
- Taxon
- riceOryza sativat0
- Material
- OE 19e0
- Treatment
- salt stresse1
- Gene
- OsAAI1e0
- Outcome
- plant height, root length and numbere1
- Direction
- opposite trendse0
- Comparator
- ZH 11e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while OsAAI1 overexpression (OE 19) showed opposite trends”
e1“Under salt stress, the mutant OsAAI1 ( osaai1 ) had significantly higher plant height, root length and number, and lower ROS accumulation than ZH 11”
t0“rice root development”
Claim · model-written summary
OE 19 had lower antioxidant enzyme activities.
- Taxon
- riceOryza sativat0
- Material
- OE 19e0
- Outcome
- antioxidant enzyme activitiese0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OE 19 also had lower antioxidant enzyme activities and higher MDA content”
t0“rice root development”
Claim · model-written summary
OE 19 had higher MDA content.
- Taxon
- riceOryza sativat0
- Material
- OE 19e0
- Outcome
- MDA contente0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OE 19 also had lower antioxidant enzyme activities and higher MDA content”
t0“rice root development”
Claim · model-written summary
After 30 and 50 days of salt stress, osaai1 sustained root growth and development whereas OE 19 was more damaged.
- Taxon
- riceOryza sativat0
- Material
- osaai1e0
- Outcome
- root growth and developmente0
- Qualifier
- after 30 and 50 days of salt stress treatmente1
- Comparator
- OE 19e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“osaai1 was able to sustain root growth and development under salt stress conditions, whereas OE 19 was more damaged”
e1“after 30 and 50 days of salt stress treatment”
t0“rice root development”
Claim · model-written summary
Nitrate application enhanced salt tolerance and root growth.
- Taxon
- riceOryza sativat0
- Treatment
- nitrate applicatione0
- Outcome
- salt tolerance, improving root growthe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nitrate application enhanced OsAAI1 is salt tolerance, improving root growth and increasing ROS scavenging enzyme activities”
t0“rice root development”
Claim · model-written summary
Nitrate application increased ROS scavenging enzyme activities.
- Taxon
- riceOryza sativat0
- Treatment
- nitrate applicatione0
- Outcome
- ROS scavenging enzyme activitiese0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nitrate application enhanced OsAAI1 is salt tolerance, improving root growth and increasing ROS scavenging enzyme activities”
t0“rice root development”
Claim · model-written summary
High-concentration KNO₃ restored the root phenotype of OE 19.
- Taxon
- riceOryza sativat0
- Material
- OE 19e0
- Treatment
- high-concentration KNO₃e0
- Outcome
- root phenotypee0
- Direction
- restorese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high-concentration KNO₃ restores the root phenotype in OE 19”
t0“rice root development”
Claim · model-written summary
OsAAI1 is interpreted to regulate ROS homeostasis through the nitrate pathway in salt tolerance.
- Taxon
- riceOryza sativat0
- Gene
- OsAAI1e0
- Outcome
- ROS homeostasise0
- Qualifier
- through the nitrate pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsAAI1 regulated ROS homeostasis through the nitrate pathway to enhance its tolerance to salt stress”
t0“rice root development”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.