Abstract
The genome of rice blast fungus (Magnaporthe oryzae) encodes 15 glycoside hydrolase 18 family chitinases. In this study, we characterized the function of an M. oryzae extracellular chitinase, MoChi1, and its interaction with a host protein, OsMBL1, a jacalin-related Mannose-Binding Lectin (MBL) in rice (Oryza sativa). Deletion of MoChi1 resulted in reduced aerial hyphal formation and reduced virulence in rice by activating the expression of defense-responsive genes. We confirmed MoChi1 interaction with rice OsMBL1 in vitro and in vivo. OsMBL1 was induced by pathogen-associated molecular patterns and M. oryzae infection. Overexpression of OsMBL1 led to activation of rice defense-responsive genes and a chitin-induced reactive oxygen species burst, thereby enhancing resistance to M. oryzae. Knockdown of OsMBL1 enhances susceptibility of rice plants to M. oryzae. Furthermore, MoChi1 suppressed chitin-induced reactive oxygen species in rice cells and competed with OsMBL1 for chitin binding. Taken together, our study reveals a mechanism in which MoChi1 targets a host lectin to suppress rice immunity.
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Claims 11
Claim · model-written summary
Deletion of MoChi1 reduced aerial hyphal formation of Magnaporthe oryzae.
- Taxon
- Oryza sativa
Oryza sativae0 - Treatment
- Deletion of MoChi1e1
- Interactor
- Magnaporthe oryzae
Pyricularia oryzaee2pathogen - Gene
- MoChi1e1
- Outcome
- aerial hyphal formatione1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa)”
e1“Deletion of MoChi1 resulted in reduced aerial hyphal formation and reduced virulence in rice by activating the expression of defense-responsive genes”
e2“rice blast fungus (Magnaporthe oryzae)”
Claim · model-written summary
Deletion of MoChi1 reduced virulence in rice.
- Taxon
- Oryza sativaOryza sativae0
- Treatment
- Deletion of MoChi1e1
- Interactor
- Magnaporthe oryzaePyricularia oryzaee2pathogen
- Gene
- MoChi1e1
- Outcome
- virulencee1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa)”
e1“Deletion of MoChi1 resulted in reduced aerial hyphal formation and reduced virulence in rice by activating the expression of defense-responsive genes”
e2“rice blast fungus (Magnaporthe oryzae)”
Claim · model-written summary
Deletion of MoChi1 activated defense-responsive gene expression in rice.
- Taxon
- Oryza sativaOryza sativae0
- Treatment
- Deletion of MoChi1e1
- Gene
- MoChi1e1
- Outcome
- defense-responsive genese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa)”
e1“Deletion of MoChi1 resulted in reduced aerial hyphal formation and reduced virulence in rice by activating the expression of defense-responsive genes”
Claim · model-written summary
MoChi1 interacts with OsMBL1 in vitro and in vivo.
- Taxon
- Oryza sativaOryza sativae0
- Interactor
- Magnaporthe oryzaePyricularia oryzaee2pathogen
- Gene
- OsMBL1e3
- Outcome
- interactione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa)”
e2“rice blast fungus (Magnaporthe oryzae)”
e3“We confirmed MoChi1 interaction with rice OsMBL1 in vitro and in vivo”
Claim · model-written summary
OsMBL1 was induced by pathogen-associated molecular patterns and Magnaporthe oryzae infection.
- Taxon
- Oryza sativaOryza sativae0
- Treatment
- pathogen-associated molecular patterns and M. oryzae infectione4
- Interactor
- Magnaporthe oryzaePyricularia oryzaee2pathogen
- Gene
- OsMBL1e4
- Outcome
- OsMBL1e4
- Direction
- inducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa)”
e2“rice blast fungus (Magnaporthe oryzae)”
e4“OsMBL1 was induced by pathogen-associated molecular patterns and M. oryzae infection”
Claim · model-written summary
Overexpression of OsMBL1 activated rice defense-responsive genes.
- Taxon
- Oryza sativaOryza sativae0
- Treatment
- Overexpression of OsMBL1e5
- Gene
- OsMBL1e5
- Outcome
- defense-responsive genese5
- Direction
- activatione5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa)”
e5“Overexpression of OsMBL1 led to activation of rice defense-responsive genes and a chitin-induced reactive oxygen species burst, thereby enhancing resistance to M. oryzae”
Claim · model-written summary
Overexpression of OsMBL1 led to a chitin-induced reactive oxygen species burst in rice.
- Taxon
- Oryza sativaOryza sativae0
- Treatment
- Overexpression of OsMBL1e5
- Gene
- OsMBL1e5
- Outcome
- chitin-induced reactive oxygen species burste5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa)”
e5“Overexpression of OsMBL1 led to activation of rice defense-responsive genes and a chitin-induced reactive oxygen species burst, thereby enhancing resistance to M. oryzae”
Claim · model-written summary
Overexpression of OsMBL1 enhanced rice resistance to Magnaporthe oryzae.
- Taxon
- Oryza sativaOryza sativae0
- Treatment
- Overexpression of OsMBL1e5
- Interactor
- Magnaporthe oryzaePyricularia oryzaee2pathogen
- Gene
- OsMBL1e5
- Outcome
- resistance to M. oryzaee5
- Direction
- enhancinge5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa)”
e2“rice blast fungus (Magnaporthe oryzae)”
e5“Overexpression of OsMBL1 led to activation of rice defense-responsive genes and a chitin-induced reactive oxygen species burst, thereby enhancing resistance to M. oryzae”
Claim · model-written summary
Knockdown of OsMBL1 enhanced rice susceptibility to Magnaporthe oryzae.
- Taxon
- Oryza sativaOryza sativae0
- Treatment
- Knockdown of OsMBL1e6
- Interactor
- Magnaporthe oryzaePyricularia oryzaee2pathogen
- Gene
- OsMBL1e6
- Outcome
- susceptibilitye6
- Direction
- enhancese6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa)”
e2“rice blast fungus (Magnaporthe oryzae)”
e6“Knockdown of OsMBL1 enhances susceptibility of rice plants to M. oryzae”
Claim · model-written summary
MoChi1 suppressed chitin-induced reactive oxygen species in rice cells.
- Taxon
- Oryza sativaOryza sativae0
- Treatment
- MoChi1e7
- Interactor
- Magnaporthe oryzaePyricularia oryzaee2pathogen
- Gene
- MoChi1e7
- Outcome
- chitin-induced reactive oxygen speciese7
- Direction
- suppressede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa)”
e2“rice blast fungus (Magnaporthe oryzae)”
e7“Furthermore, MoChi1 suppressed chitin-induced reactive oxygen species in rice cells and competed with OsMBL1 for chitin binding”
Claim · model-written summary
MoChi1 competed with OsMBL1 for chitin binding.
- Taxon
- Oryza sativaOryza sativae0
- Gene
- MoChi1e7
- Outcome
- chitin bindinge7
- Qualifier
- competed with OsMBL1e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in rice (Oryza sativa)”
e7“Furthermore, MoChi1 suppressed chitin-induced reactive oxygen species in rice cells and competed with OsMBL1 for chitin binding”
The same questions in other papers
loss of function · Pyricularia oryzae · Oryza sativa (rice)Positive and negative results
40 papers in the same direction as this paper · 26 in the opposite direction
- negative
- negative
- negative
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See all 67 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.